Pigment Production From Bacteria Isolated From Whey
Yıl 2024,
Cilt: 28 Sayı: 3, 602 - 609, 30.06.2024
Sude Çardak
,
İlke Karakaş
,
Nurcihan Hacıoğlu Doğru
Öz
Environmental pollution and population growth necessitate more efficient production processes. Organic whey, which is a low-cost substrate for the food industry, constitutes a promising raw material with its low cost and chemical content for biotechnological processes. This study investigated the pigment production capabilities of bacteria isolated from whey, an industrial waste. Among the isolated bacteria, 4 were determined to be effective pigment producing bacteria. The pigment was extracted from 4 isolates. Pigment characterization was performed by UV spectrophotometer (OD470-OD580) and Fourier Transform Infrared Spectroscopy (FTIR). As a result of the spectrum scanning, it was determined that all pigments gave the maximum absorbance value in the range of 500 nm to 505 nm. In FTIR analysis, all extracted pigments showed characteristic absorption bands of carotenoids between 400 nm and 520 nm. The FTIR peaks obtained from 1469 cm-1 and 1726 cm-1 regions are known as the fingerprint regions of microbial pigments for biorecolorants. When the results obtained in our study are compared with the literature data, the absorbance values obtained show that the pigment produced is carotenoid and its derivative.
Etik Beyan
Bu çalışma için etik kurul iznine gerek yoktur.
Destekleyen Kurum
Bu çalışma TÜBİTAK-2209 Biyoloji Bilimleri Lisans Öğrenci Bursu kapsamında desteklenen bir projedir.
Proje Numarası
TÜBİTAK 2209A
Kaynakça
- [1] F. M. Said, N. F. B. Hamid, “Optimization of Red Pigment Production by Monascus purpureus FTC 5356 Using Response Surface Methodology,” IIUM Engineering Journal, vol. 19, no. 1, pp. 34-47, 2018.
- [2] G. Sharmila, B. Nidhi, C. Muthukumaran, “Sequential Statistical Optimization of Red Pigment Production by Monascus purpureus (MTCC 369) Using Potato Powder,” Industrial Crops and Products, vol. 44, pp. 158-164, 2013.
- [3] A. Kumar, H. S. Vishwakarma, J. Singh, S. Dwivedi, M. Kumar, “Microbial Pigments: Production and Their Applications in Various Industries,” International Journal of Pharmaceutical and Chemical Sciences, vol. 5, no. 1, pp. 203-212, 2015.
- [4] C. K. Venil, P. Lakshmanaperumalsamy, “An Insightful Overview on Microbial Pigment, Prodigiosin,” Electronic Journal of Biology, vol. 5, no. 3, pp. 49-61, 2009.
- [5] G. Y. Liu, V. Nizet, “Color Me Bad: Microbial Pigments as Virulence Factors,” Trends in Microbiology, vol. 17 no.9, pp. 842-966, 2009.
- [6] D. Pleissner, Q. Qi, C. Gao, C. P. Rivero, C. Webb, C. S. K. Lin, J. “Venus, Valorization of Organic Residues for The Production of Added Value Chemicals: A Contribution to The Bio-Based Economy,” Biochemical Engineering Journal, vol. 116, pp. 3-16, 2016.
- [7] J. Liu, Y. Luo, T. Guo, C. Tang, X. Chai, W. Zhao, Q. Lin, “Costeffective Pigment Production by Monascus Purpureus Using Rice Straw Hydrolysate as Substrate in Submerged Fermentation”, Journal of Bioscience and Bioengineering, vol. 129, no. 2, pp. 229-236, 2020.
- [8] P. Atalay, “Microbial Production of Color Pigment from Waste Beer,” Master Thesis, Ege University, İzmir, Türkiye, 2020.
- [9] M. S. Şılbır, “Production of Monascus Color Pigments from Beer Waste and Determination of Their Stability,” Ege University, İzmir, Türkiye, 2019.
- [10] A. Kantifedaki, V. Kachrimanidou, A. Mallouchos, S. Papanikolaou, A. A. Koutinas, “Orange Processing Waste Valorisation for the Production of Bio-Based Pigments Using the Fungal Strains Monascus Purpureus and Penicillium Purpurogenum, Journal of Cleaner Production, vol. 185, pp. 882-890, 2018.
- [11] S. T. Silveira, D. J. Daroit, V. Sant’Anna, A. Brandelli, “Stability Modeling of Red Pigments Produced by Monascus purpureus In Submerged Cultivations with Sugarcane Bagasse,” Food and Bioprocess Technology, vol. 6, no. 4, pp. 1007-1014, 2013.
- [12] R. T. Hilares, R. A. de Souza, P. F. Marcelino, S. S. da Silva, G. Dragone, S. I. Mussatto, J. C. Santos, “Sugarcane Bagasse Hydrolysate as A Potential Feedstock for Red Pigment Production by Monascus ruber,” Food Chemistry, vol. 245, pp. 786-791, 2018.
- [13] N. Singh, G. Goel, N. Singh, K. Pathak, D. Kaushik, “Modeling the Red Pigment Production by Monascus purpureus MTCC 369 by Artificial Neural Network Using Rice Water Based Medium,” Food Bioscience, vol. 11, pp. 17-22, 2015.
- [14] P. Srivastav, V. K. Yadav, S. Govindasamy, M. Chandrasekaran, “Red Pigment Production by Monascus purpureus Using Sweet Potato-Based Medium in Submerged Fermentation,” Nutrafoods, vol. 14, no. 3, pp. 159-167, 2015.
- [15] O. Yerlikaya, Ö. Kınık, N. Akbulut, “Functional Properties of Whey and Milk Beverages Produced Using Whey,” Gıda, vol. 35, no. 4, pp. 289-296, 2010.
- [16] J. Hausjell, M. Miltner, C. Herzig, A. Limbeck, Z. Saracevic, E. Saracevic, O. Spadiut, “Valorisation of Cheese Whey As Substrate and Inducer for Recombinant Protein Production in E. coli HMS174 (DE3),” Bioresource Technology Reports, vol. 8, pp. 100340, 2019.
- [17] S. Yıldırım, “Production of Pigment by Isolated Microorganisms from Hydrocarbon Pollution of Soil,” Master Thesis, Eskişehir Osmangazi University, Eskişehir, Türkiye, 2014.
- [18] B. Zhou, J. Wang, Y. Pu, M. Zhu, S. Liu, S. Liang, “Optimization of Culture Medium for Yellow Pigments Production with Monascusanka Mutant Using Response Surface Methodology,” Europan Food Research Technology, vol. 228, pp. 895–901, 2008.
- [19] P. Indra Arulselvi, S. Umamaheswari, G. Ranandkumar Sharma, C. Karthik, C. Jayakrishna, “Screening of Yellow Pigment Producing Bacterial Isolates from Various Eco-Climatic Areas and Analysis of the Carotenoid Produced by the Isolate,” Journal of Food Processing Technology, vol. 5, pp. 292, 2014.
- [20] N. Trivedi, S. Tandon, A. Dubey, “Fourier Transform Infrared Spectroscopy (FTIR) Profiling of Red Pigment Produced by Bacillus subtilis PD5,” African Journal of Biotechnology, vol. 16, no. 27, pp. 1507-1512, 2017.
- [21] W. A. Ahmad, W. Y. W. Ahmad, Z. A. Zakaria, N. Z. Yusof, “Isolation of Pigment-Producing Bacteria and Characterization of the Extracted Pigments,” Application of Bacterial Pigments as Colorant, pp. 25–44, 2011.
- [22] N. Órdenes-Aenishanslins, G. Anziani-Ostuni, M. Vargas-Reyes, J. Alarcón, A. Tello, J. Pérez-Donoso, “Pigments from UV-Resistant Antarctic Bacteria as Photosensitizers in Dye Sensitized Solar Cells,” Journal of Photochemistry and Photobiology B: Biology, vol. 162, pp. 707-714, 2016.
- [23] P. Atalay, S. Sargın, Y. Goksungur, Y., “Utilization of Residual Beer for Red Pigment Production by Monascus purpureus in Submerged Fermantation,” Fresenius Environmental Bulletin, vol. 29, pp. 1025-1034, 2020.
Yıl 2024,
Cilt: 28 Sayı: 3, 602 - 609, 30.06.2024
Sude Çardak
,
İlke Karakaş
,
Nurcihan Hacıoğlu Doğru
Proje Numarası
TÜBİTAK 2209A
Kaynakça
- [1] F. M. Said, N. F. B. Hamid, “Optimization of Red Pigment Production by Monascus purpureus FTC 5356 Using Response Surface Methodology,” IIUM Engineering Journal, vol. 19, no. 1, pp. 34-47, 2018.
- [2] G. Sharmila, B. Nidhi, C. Muthukumaran, “Sequential Statistical Optimization of Red Pigment Production by Monascus purpureus (MTCC 369) Using Potato Powder,” Industrial Crops and Products, vol. 44, pp. 158-164, 2013.
- [3] A. Kumar, H. S. Vishwakarma, J. Singh, S. Dwivedi, M. Kumar, “Microbial Pigments: Production and Their Applications in Various Industries,” International Journal of Pharmaceutical and Chemical Sciences, vol. 5, no. 1, pp. 203-212, 2015.
- [4] C. K. Venil, P. Lakshmanaperumalsamy, “An Insightful Overview on Microbial Pigment, Prodigiosin,” Electronic Journal of Biology, vol. 5, no. 3, pp. 49-61, 2009.
- [5] G. Y. Liu, V. Nizet, “Color Me Bad: Microbial Pigments as Virulence Factors,” Trends in Microbiology, vol. 17 no.9, pp. 842-966, 2009.
- [6] D. Pleissner, Q. Qi, C. Gao, C. P. Rivero, C. Webb, C. S. K. Lin, J. “Venus, Valorization of Organic Residues for The Production of Added Value Chemicals: A Contribution to The Bio-Based Economy,” Biochemical Engineering Journal, vol. 116, pp. 3-16, 2016.
- [7] J. Liu, Y. Luo, T. Guo, C. Tang, X. Chai, W. Zhao, Q. Lin, “Costeffective Pigment Production by Monascus Purpureus Using Rice Straw Hydrolysate as Substrate in Submerged Fermentation”, Journal of Bioscience and Bioengineering, vol. 129, no. 2, pp. 229-236, 2020.
- [8] P. Atalay, “Microbial Production of Color Pigment from Waste Beer,” Master Thesis, Ege University, İzmir, Türkiye, 2020.
- [9] M. S. Şılbır, “Production of Monascus Color Pigments from Beer Waste and Determination of Their Stability,” Ege University, İzmir, Türkiye, 2019.
- [10] A. Kantifedaki, V. Kachrimanidou, A. Mallouchos, S. Papanikolaou, A. A. Koutinas, “Orange Processing Waste Valorisation for the Production of Bio-Based Pigments Using the Fungal Strains Monascus Purpureus and Penicillium Purpurogenum, Journal of Cleaner Production, vol. 185, pp. 882-890, 2018.
- [11] S. T. Silveira, D. J. Daroit, V. Sant’Anna, A. Brandelli, “Stability Modeling of Red Pigments Produced by Monascus purpureus In Submerged Cultivations with Sugarcane Bagasse,” Food and Bioprocess Technology, vol. 6, no. 4, pp. 1007-1014, 2013.
- [12] R. T. Hilares, R. A. de Souza, P. F. Marcelino, S. S. da Silva, G. Dragone, S. I. Mussatto, J. C. Santos, “Sugarcane Bagasse Hydrolysate as A Potential Feedstock for Red Pigment Production by Monascus ruber,” Food Chemistry, vol. 245, pp. 786-791, 2018.
- [13] N. Singh, G. Goel, N. Singh, K. Pathak, D. Kaushik, “Modeling the Red Pigment Production by Monascus purpureus MTCC 369 by Artificial Neural Network Using Rice Water Based Medium,” Food Bioscience, vol. 11, pp. 17-22, 2015.
- [14] P. Srivastav, V. K. Yadav, S. Govindasamy, M. Chandrasekaran, “Red Pigment Production by Monascus purpureus Using Sweet Potato-Based Medium in Submerged Fermentation,” Nutrafoods, vol. 14, no. 3, pp. 159-167, 2015.
- [15] O. Yerlikaya, Ö. Kınık, N. Akbulut, “Functional Properties of Whey and Milk Beverages Produced Using Whey,” Gıda, vol. 35, no. 4, pp. 289-296, 2010.
- [16] J. Hausjell, M. Miltner, C. Herzig, A. Limbeck, Z. Saracevic, E. Saracevic, O. Spadiut, “Valorisation of Cheese Whey As Substrate and Inducer for Recombinant Protein Production in E. coli HMS174 (DE3),” Bioresource Technology Reports, vol. 8, pp. 100340, 2019.
- [17] S. Yıldırım, “Production of Pigment by Isolated Microorganisms from Hydrocarbon Pollution of Soil,” Master Thesis, Eskişehir Osmangazi University, Eskişehir, Türkiye, 2014.
- [18] B. Zhou, J. Wang, Y. Pu, M. Zhu, S. Liu, S. Liang, “Optimization of Culture Medium for Yellow Pigments Production with Monascusanka Mutant Using Response Surface Methodology,” Europan Food Research Technology, vol. 228, pp. 895–901, 2008.
- [19] P. Indra Arulselvi, S. Umamaheswari, G. Ranandkumar Sharma, C. Karthik, C. Jayakrishna, “Screening of Yellow Pigment Producing Bacterial Isolates from Various Eco-Climatic Areas and Analysis of the Carotenoid Produced by the Isolate,” Journal of Food Processing Technology, vol. 5, pp. 292, 2014.
- [20] N. Trivedi, S. Tandon, A. Dubey, “Fourier Transform Infrared Spectroscopy (FTIR) Profiling of Red Pigment Produced by Bacillus subtilis PD5,” African Journal of Biotechnology, vol. 16, no. 27, pp. 1507-1512, 2017.
- [21] W. A. Ahmad, W. Y. W. Ahmad, Z. A. Zakaria, N. Z. Yusof, “Isolation of Pigment-Producing Bacteria and Characterization of the Extracted Pigments,” Application of Bacterial Pigments as Colorant, pp. 25–44, 2011.
- [22] N. Órdenes-Aenishanslins, G. Anziani-Ostuni, M. Vargas-Reyes, J. Alarcón, A. Tello, J. Pérez-Donoso, “Pigments from UV-Resistant Antarctic Bacteria as Photosensitizers in Dye Sensitized Solar Cells,” Journal of Photochemistry and Photobiology B: Biology, vol. 162, pp. 707-714, 2016.
- [23] P. Atalay, S. Sargın, Y. Goksungur, Y., “Utilization of Residual Beer for Red Pigment Production by Monascus purpureus in Submerged Fermantation,” Fresenius Environmental Bulletin, vol. 29, pp. 1025-1034, 2020.