İki Doğal Polisakkaritin Hiyalüronidaz, Kollajenaz ve Elastaz İnhibitör Potansiyellerinin Araştırılması ve Antimikrobiyal, Antioksidan ve Homeostatik Aktivitelerinin Karşılaştırmalı Olarak Değerlendirmesi

Yıl 2018, Cilt: 22 Sayı: 3, 1182 - 1189, 20.09.2018

Rukiye Boran , Aysel Ugur , Nurdan Sarac

https://doi.org/10.19113/sdufenbed.471994

Cited By: 7

Öz

Bu çalışmanın amacı, kitre zamkı (TG) ve
keçiboynuzu zamkı (LBG)'nin antibakteriyel, antioksidan ve homeostatik
aktiviteleri ile birlikte yara iyileşmesi için önemli olan hiyalüronidaz,
kollajenaz ve elastaz inhibitör etkilerini araştırmaktır. Antimikrobiyal
aktiviteleri dört bakteriye karşı test edilerek, antioksidan aktiviteleri
1,1-difenil-2-pikrilhidrazil (DPPH), hidrojen peroksit (H₂0₂)
radikal temizleme ve β-karoten ağartma deneyleri ile tespit edilmiştir. Homeostatik
etki Protrombin Zamanı (PT) ve Aktive Edilen Kısmi Tromboplastin Zamanı (aPTT)
test parametreleri ile değerlendirilmiştir. Yara iyileştirme potansiyelleri
ise, hiyalüronidaz, kollajenaz ve elastaz inhibisyonu ile belirlenmiştir. TG, Pseudomonas aeruginosa ATCC27853 ve Escherichia coli ATCC25922'ye karşı
antibakteriyel aktivite göstermiştir. Sonuçlar TG ve LBG'nin DPPH temizleme
(sırasıyla %21.0 ve %17.6) ve H₂O₂ radikal temizleme
(sırasıyla %59.4 ve %79.0) aktiviteleri de dahil olmak üzere antioksidan
özelliklere sahip olduğunu göstermiştir. Polisakkaritler, PT ve aPTT'de önemli
azalma göstermiştir. Test edilen iki polisakkarit arasından LBG, 10 mg/mL
konsantrasyonda, önemli hiyalüronidaz ve kollajenaz inhibisyon aktivitesi
göstermiştir. Bu bulgular, bu doğal polisakkaritlerin yara iyileşmesini
desteklemek için kullanılabileceğini göstermektedir.

Anahtar Kelimeler

Polisakkarit, Enzim inhibisyonu, Antibakteriyel, Antioksidan, Homeostatik

Investigation of Hyaluronidase, Collagenase and Elastase Inhibitory Potentials and Comparative Evaluation of the Antimicrobial, Antioxidant and Homeostatic Activities of Two Natural Polysaccharides

Öz

The aim of this study was to investigate the
hyaluronidase, collagenase and elastase inhibitory effects, which play important
role for wound healing, together with the antibacterial, antioxidant and
homeostatic activities of tragacanth gum (TG)
and locust bean gum (LBG). The
antimicrobial activities were tested against four bacteria and the antioxidant
activities were estimated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen
peroxide (H₂O₂) radical
scavenging and β-carotene bleaching assays. Homeostatic effect was
evaluated with the Prothrombin Time (PT)
and Activated Partial Thromboplastin Time (aPTT)
test parameters. The wound healing potentials were determined with the
inhibition of hyaluronidase, collagenase and elastase enzymes. The TG showed
antibacterial activity against Pseudomonas
aeruginosa ATCC27853 and Escherichia coli ATCC25922. The results showed that TG and LBG possessed
antioxidant properties including DPPH scavenging  (21.0% and 17.6%,
respectively)  and H₂O₂ radical scavenging (59.4% and 79.0%,
respectively) activities. The polysaccharides displayed significantly reducing PT and aPTT results.
Between the two tested polysaccharides LBG showed significant
hyaluronidase and collagenase inhibition activity at 10 mg/mL concentration. These findings show that these natural polysaccharides can be used to
support of wound healing.

Anahtar Kelimeler

Polysaccharide, Enzyme inhibition, Antibacterial, Antioxidant, Homeostatic

Kaynakça

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