Çeşitli Antiseptik Taşıyıcılarla Karıştırılan Kalsiyum Hidroksitin Enterococcus faecalis üzerindeki Antimikrobiyal Etkinliği

Yıl 2017, Cilt: 4 Sayı: 2, 44 - 51, 28.07.2017

Makbule Bilge Akbulut , Mehmet Burak Güneşer , Ayçe Ünverdi Eldeniz

https://doi.org/10.15311/1441.308558

Öz

Amaç: Bu in vitro çalışmanın amacı farklı
antiseptik taşıyıcı-kalsiyum hidroksit (KH) kombinasyonlarının Enterococcusfaecalis(E.
faecalis) ile enfekte kök kanallarındaki antibakteriyel etkinliğini dentin
blok modeli kullanarak test etmektir.

Gereç ve Yöntemler: Kalsiyum
hidroksit ile karıştırılmak üzere kullanılan taşıyıcılar gliserin distile su
karışımı (7:1), oktenidinhidroklorit, %2’lik klorheksidinglukonat, %15’lik
setrimit + %1.5’luk klorheksidinglukonat, %5.25’lik sodyum hipoklorittir.
Altmış adet çekilmiş insan dişi örnekleri hazırlandı. Elli-beş tane kök
segmenti E. faecalis ile 2 hafta boyunca enfekte edildi. Örnekler 5 deney
grubu (n=10) ve pozitif kontrol grubuna (n=5) ayrıldı. Antiseptik-kalsiyum
hidroksit karışımları 7 gün boyunca kök kanallarında bekletildi. Beş örnek
sterilliği kontrol etmek için kullanıldı (negatif kontrol). Gates
glidden frezler (# 3, 4, 5) yardımıyla kanal duvarlarından elde edilen dentin
örnekleri fosfat-tamponlu salin (PBS) ve cam boncuk içeren şişelere aktarıldı
ve 30 sn vortekslendi.  Bakteri içeren
PBS’e seri dilüsyon yapıldı. Her bir dilüsyondan 25 μL halinde damlalar tripton
soya agar plakları üzerine ekildi ve 37°C ‘de 48 saat inkübe edildi. Görünür koloni
sayıları incelendi ve log₁₀ değerlerine dönüştürüldü. İstatistiksel
analiz için Kruskal-Wallis ve Mann Whitney-U testleri kullanıldı.

Bulgular: Negatif
kontrol grubunda bakteri üremesi görülmezken pozitif  kontrol grubunun bütün
örneklerinde bakteri üremesi gözlemlenmiştir (log₁₀3,47 CFU ml^-1).
Deney grupları arasında istatistiksel olarak anlamlı bir fark bulunmamıştır (p>0.05).  KH +%2 klorheksidin ve KH+
setrimit-klorheksidin gruplarında E. faecalis biyofilmi tamamen elimine
edilmiştir.  

Sonuçlar: Setrimit-klorheksidin,
oktenidin hidroklorit ve sodyum hipoklorit gibi çeşitli antiseptik ajanlar
kalsiyum hidroksit ile karıştırılarak kullanıldığında kök kanallarından E.
faecalis’i elimine etmede etkili bulunmuştur. 

Anahtar Kelimeler: antimikrobiyal,  E.
faecalis,  kök dentini,  kanal-içi medikament

Anahtar Kelimeler

antimikrobiyal, E. faecalis, kök dentini, kanal-içi medikament

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