Classification of Bean Genotypes by Protein Profiles

Yıl 2016, Cilt: 30 Sayı: 1, 29 - 33, 14.12.2016

Ali Kahraman , Ercan Ceyhan , Tuna Uysal , Zuhal Ozkan , Ela Nur Şimşek Sezer Meryem Bozkurt

Öz

Conservation of biodiversity is the main concept to provide sustainability. There are various methods to identify diversity of living organisms. SDS-PAGE is one the most reliable   method to classification of plants.

Dry bean is the most important pulse crop over the world besides Konya has the highest production in Turkey which is expected as the widest genetic variation among the genotypes. In the present study, a total of 22 bean genotypes (20 populations from Konya and 2 certificated varieties) were used to obtain the protein patterns by using SDS-PAGE methods. Diversity of the genotypes and their relatives were evaluated with dendrogram. Results implicated that protein profiles of the investigated beans provided a clear classification by view of selection criteria. Similarity dendrogram presented two main groups that showed the ranges nearly 20-75% and 50-90%, respectively. Furthermore, two of the local populations (PV 19 and PV 20) showed a subgroup with certified varieties (Akman-98 and Gina).

A comparison of SDS-PAGE method showed that, the method could be used to find solutions for the taxonomic and evolutionary problems of the bean genotypes. Therefore, this method may be a useful tool for plant breeders to simplification of selection and classification on genotypes.

Anahtar Kelimeler

Cluster, dendrogram; diversity; Phaseolus vulgaris; protein; sds page.

Kaynakça

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