Piroglutamil Peptidlerin Farelerde Skopolaminle Oluşturulmuş Öğrenme-Bellek Bozukluğu Üzerine Etkisi

Yıl 2023, Cilt: 13 Sayı: 3, 446 - 455, 30.09.2023

İbrahim Uygun , Şeyma Nur Başarır Bozkurt , Mohammad Al Yousef , Mohammed Omer Khalid Mohammed , Pelin Tanyeri , Firuzan Akar , Bekir Faruk Erden , Oguz Mutlu

https://doi.org/10.31832/smj.1239798

Öz

Amaç: Önceki çalışmalarımızda adipokinetik hormonun (AKH) farelerde antidepresan, anksiyolitik, analjezik etkilerini, nörotrofik faktörleri ve nörojenezi artırdığını gösterdik. Önceki çalışmamızda, AKH’nun sıçanlara intraperitoneal uygulanmasından sonra diğer çalışmalarla desteklendiği şekilde piroglutamil peptidleri içeren AKH metabolitlerinin kan-beyin bariyerini geçtiğini hipotez ettik.
Gereç ve Yöntemler: Bu çalışmada, iki piroglutamil peptidin öğrenme-bellek üzerine etkisini modifiye yükseltilmiş artı labirent testinde (mYAL) ve pasif sakınma testinde hem naif farelerde hem de skopolaminle indüklenen bellek bozukluğu üzerine etkisini incelemeyi amaçladık. Skopolamin (1 mg/kg) ile piroglutamil peptid olarak piroglutamik asid-valin (pGlu-Val; 10 ve 20 mg/kg), piroglutamik asid-lösin (pGlu-Leu; 10 ve 20 mg/kg) kullandık.
Bulgular: mYAL testinde dipeptidlerin naif farelerde geçiş süresi-2 üzerine anlamlı etkisi yoktu. Skopolamin kısmi olarak ikinci denemede geçiş süresi-2’yi artırırken, bu etki pGlu-Leu (10 ve 20 mg/kg; p<0,01) tarafından anlamlı şekilde tersine çevrildi fakat pGlu-Val etkisizdi. Pasif sakınma testinde dipeptidlerin naif farelerde retansiyon latansı üzerine anlamlı etkisi yoktu. Skopolamin kontrol grubuna gore retansiyon latansını kısmen azaltırken dipeptidlerin hiçbiri skopolamin grubunun retansiyon latansını tersine çevirmedi.
Sonuç: Sonuçta, piroglutamil peptidler naif farelerde öğrenme-bellek üzerine etki göstermedi. pGlu-Leu mYAL testinde skopolaminle indüklenen öğrenme bozukluğu üzerine düzeltici etki gösterirken, her iki peptid pasif sakınma testinde skopolaminle indüklenen bellek bozukluğu üzerine etki göstermedi.

Anahtar Kelimeler

Fare, Modifiye yükseltilmiş artı labirent, Öğrenme-bellek, Pasif sakınma, Piroglutamil peptidler, Learning-memory, Mice, Modified elevated plus maze, Passive avoidance, Pyroglutamyl peptides

Effects Of Pyroglutamyl Peptides On Scopolamine Induced Learning-Memory Impairment In Mice

Öz

Objective: Adipokinetic hormone (AKH) had antidepressant, anxiolytic and analgesic effects in mice and increased neurotrophic factors and neurogenesis in mice. Metabolites of AKH, including pyroglutamyl peptides, pass the blood-brain barrier which is supported by other studies.
Materials and Methods: We aimed to investigate effects of two pyroglutamyl peptides on learning and memory in modified elevated plus maze (mEPM) and passive avoidance tests both in naive mice and in scopolamine-induced memory deterioration. We used scopolamine (1 mg/kg) and pyroglutamic acid-valine (pGlu-Val; 10 and 20 mg/kg), pyroglutamic acid-leucine (pGlu-Leu; 10 and 20 mg/kg) as pyroglutamyl peptides.
Results: In mEPM test there was no significant effect of dipeptides on transfer latency-2 in naive mice. Scopolamine partially increased transfer latency-2 in second trial and this effect was significantly reversed by pGlu-Leu (10 and 20 mg/kg; p<0.01) although pGlu-Val had no effect. In passive avoidance test none of the dipeptides reversed retention latency of scopolamine group.
Conclusion: We found that pyroglutamyl peptides had no effect on learning and memory in naive mice. pGlu-Leu had improving effect on scopolamine induced learning impairment in the mEPM test while both of the peptides had no effect on scopolamine induced memory impairment in the passive avoidance test.

Anahtar Kelimeler

Fare, Modifiye yükseltilmiş artı labirent, Öğrenme-bellek, Pasif sakınma, Piroglutamil peptidler, Learning-memory, Mice, Modified elevated plus maze, Passive avoidance, Pyroglutamyl peptides

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