Cannabis Sativa L. (Kenevir)’de İn Vitro Mikroçoğaltım

Yıl 2020, Cilt: 3 Sayı: 2, 54 - 62, 14.12.2020

Cennet Yaman

Öz

Cannabis sativa (Kenevir) birçok endüstriyel alanda kullanılan bir bitkidir. Dioik (son yıllarda geliştirilen monoik çeşitler) ve yabancı döllenen bir bitki olması, ıslah çalışmalarında ve belirli bir amaca yönelik yetiştiricilikte birçok sorun yaşatmaktadır. Bu yüzden ana ve baba birey olarak kullanılacak genotiplerin klonal çoğaltılması büyük öneme sahiptir. Klonal çoğaltım için biyoteknolojik yöntem olan mikroçoğaltım uygulaması başarılı sonuçlar vermektedir. Daha kısa sürede aynı gen dizilimine sahip birçok bitki üretimi sunmaktadır. Bu çalışmada, kenevir üzerinde yapılmış mikroçoğaltım için eksplant kaynağı ve onun sterilizasyonu, kullanılan besin ortamları, büyüme düzenleyicileri ve köklendirme araştırmaları incelenmiş ve en uygun ortamlar tespit edilmiştir. Gelecekte, ıslah çalışmaları ve amaca uygun sera veya açık alandaki yetiştiricilik işlemleri için uygun klonların çoğaltılmasında yol gösterici olacaktır.

Anahtar Kelimeler

Cannabis sativa, in vitro, mikroçoğaltım

In Vitro Micropropagation of Cannabis Sativa L. (Hemp)

Öz

Cannabis sativa (Hemp) is a plant used in many industrial areas. As hemp is a dioecious (and monoic varieties developed in recent years) and allogamy plant, it causes many problems in breeding and cultivation for a specific purpose. For this reason, the clonal reproduction of genotypes to be used as a parent is of great importance. Micropropagation application, which is a biotechnological method for clonal reproduction, gives successful results. Micropropagation offers the production of many plants with the same gene sequence in a shorter time. In this study, previous studies about explant source and its sterilization, nutrient media used, growth regulators and rooting for hemp micropropagation have been examined, and the most suitable micropropagation conditions of hemp were determined. In the future, this study will guide the reproduction of suitable clones for breeding and greenhouse or open field cultivation.

Anahtar Kelimeler

Cannabis sativa, in vitro, micropropagation

Kaynakça

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