Antimicrobial Activity of Ethanol and Methanol Extracts of Suillus bovinus (L.) Roussel Mushroom Collected from Selçuk University Campus

Yıl 2024, Cilt: 50 Sayı: 2, 60 - 65, 01.10.2024

Erdoğan Güneş

https://doi.org/10.35238/sufefd.1522776

Öz

The aim of this study is to determine the antimicrobial activity of ethanol and methanol extracts of Suillus bovinus mushroom against some standard pathogenic microorganisms. Broth microdilution method was used to detect antimicrobial activity. It was determined that methanol and ethanol extract of S. bovinus showed activity against gram-positive Staphylococcus aureus and Sarcina lutea and gram-negative Salmonella enteritidis bacteria at a dose of 6.25 mg/ml. It was detected that both extracts had an antibacterial effect against gram-negative Pseudomonas aeruginosa at a dose of 3.12 mg/ml. It was determided that both extracts were ineffective against Escherichia coli and Klebsiella pneumoniae bacteria. The strain in which methanol and ethanol extracts showed the highest activity was the gram-positive Bacillus cereus strain, with MIC (Minimum Inhibition Concentration) values of 0.19 mg/ml and 0.78 mg/ml, respectively. Additionally, the study showed that neither extract had an antifungal effect against the Candida albicans yeast strain. As a result, it is thought that the S. bovinus fungus has antibacterial activity and that the antimicrobial compounds obtained from this species with further studies will be a potential source for the development of new drugs and therapeutic agents.

Anahtar Kelimeler

Antimicrobial activitiy, Broth microdilütion, Mushroom extracts, Suillus bovinus

Selçuk Üniversitesi Kampüsünden Toplanan Suillus bovinus (L.) Roussel Mantarının Etanol ve Metanol Özütlerinin Antimikrobiyal Aktivitesi

Öz

Bu çalışmanın amacı Suillus bovinus mantarının etanol ve metanol özütlerinin bazı standart patojen mikoorganizmalara karşı antimikrobiyal aktivitesini belirlemektir. Antimikrobiyal aktiviteyi saptamak için sıvı mikrodilüsyon metodu kullanılmıştır. S. bovinus'un metanol ve etanol özütünün, gram pozitif Staphylococcus aureus ve Sarcina lutea, gram negatif Salmonella enteritidis bakterilerine karşı 6.25 mg/ml dozda antibakteriyel aktivite gösterdiği tespit edilmiştir. Her iki özütün gram negatif Pseudomonas aeruginosa’ya ise 3.12 mg/ml dozunda antibakteriyal etki gösterdiği saptanmıştır. Escherichia coli ve Klebsiella pneumoniae bakterilerine karşı her iki özütün de etkisiz olduğu belirlenmiştir. Metanol ve etanol özütü sırasıyla 0.19 mg/ml ve 0.78 mg/ml MİK (Minimum İnhibisyon Konsantrasyonu) değeriyle en yüksek aktivite gösterdikleri suş ise gram pozitif Bacillu cereus suşu olmuştur. Ayrıca yapılan çalışmada Candida albicans maya suşuna karşı her iki özütün antifungal bir etki göstermediği görülmüştür. Sonuç olarak çalışmada, S. bovinus mantarının antibakteriyal aktiviteye sahip olduğu ve ileriki çalışmalarla bu türden elde edilen antimikrobiyal bileşiklerin, yeni ilaçların ve terapötik ajanların geliştirilmesi için potansiyel bir kaynak olacağı düşünülmektedir.

Anahtar Kelimeler

Antimikrobiyal aktivite, Mantar ekstraktları, Sıvı mikrodilüsyon, Suillus bovinus

Etik Beyan

Bu çalışmanın hazırlanma sürecinde bilimsel ve etik ilkelere uyulduğu ve yararlanılan tüm çalışmaların kaynakçada belirtildiği beyan olunur

Teşekkür

Çalışmada kullanılan mantarın tür teşhisini yapan ve her türlü desteklerini sunan Dr. Öğr. Ü. Sinan ALKAN’a teşekkürlerimi sunarım.

Kaynakça

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