Isolation and identification of Streptomyces species isolated from forest area (Sakarya City) and determination the production of some extracellular enzyme

Yıl 2019, Cilt: 7 Sayı: 1, 64 - 71, 22.07.2019

Ömrüye Özok , Kerem Özdemir

https://doi.org/10.33409/tbbbd.595174

Cited By: 2

Öz

In this study, forest area located in the
province of Sakarya city soil bacteria of the genus Streptomyces isolated and made computer-assisted diagnosis and
various enzyme activities of the isolates were determined. All isolates of
phenotypic and biochemical characteristics of the data being transmitted to the
computer program determined IDENTAX, diagnosis is made by determining their
position in the genus. Isolates in the color group analysis based on aerial and
substrate mycelium color is divided into four main groups. Diagnostic test
results of Streptomyces isolates are
completed by a computer program MVSP 3.2and were created similarity dendograms.
In addition, all isolates protease, amylase, lipase, cellulase and asparaginase
enzyme activity was determined.

Anahtar Kelimeler

Streptomyces, Extracellular enzyme, characterization

Sakarya ili ormanlık alan topraklarından Streptomyces türlerinin izolasyonu, karakterizasyonu ve bazı ekstraselüler enzimlerin üretiminin belirlenmesi

Öz

Bu çalışmada, Sakarya ili sınırlarında
bulunan ormanlık alan topraklarından Streptomyces
cinsi bakterileri izole edilerek bilgisayar yardımıyla teşhisleri yapılmış ve
izolatların amilaz, selülaz, L-asparaginaz, proteaz ve lipaz enzimlerinin
aktiviteleri belirlenmiştir. Yapılan renk grubu analizinde izolatlar havasal ve
misel rengi esas alınarak 4 ana gruba ayrılmıştır. Teşhisleri tamamlanan Streptomyces izolatlarının test
sonuçları MVSP 3.2 bilgisayar programı ile birbirleri arasındaki benzerlik
dendogramları oluşturulmuştur. Tüm izolatların fenotipik ve biyokimyasal
karakterleri belirlenerek IDENTAX bilgisayar programına bu veriler aktarılmış,
cins içerisindeki pozisyonları belirlenerek teşhisleri yapılmıştır. Bu
teşhislere göre 80 izolattan 49’unun Streptomyces
exfoliatus, 14’ünün Streptomyces
atroolivaceus, 1’i Streptomyces
albidoflavus,1’i Streptomyces
chromofuscus, 1’i de Streptomyces
purpureus olduğu belirlenmiştir. Ayrıca tüm izolatların proteaz, amilaz,
lipaz, selülaz ve asparaginaz enzim aktiviteleri belirlenmiştir.

Anahtar Kelimeler

Streptomyces, ekstraselüler enzim, karakterizasyon

Kaynakça

• Atalan E, Manfio GP, Ward AC, Kroppenstedt RM, Goodfellow M. 2000. Biosystematic studied on novel Streptomycetes from soil. Antonie van Leeuwenhoek 77:337-353.
• Atalan E. 1995. Identification of Streptomyces isolated from environmental soil samples using rapid enzyme data. Journal of Environmental Science and Health Part A 30(6):1133-1143.
• Aygan A, Arikan B, Korkmaz H, Dinçer S, Çolak O. 2008. Highly thermostable and alkaline α-amylase from a halotolerant-alkaliphilic Bacillus sp. AB68. Braz. J. Microbiol. 39(3):547-53.
• Demain AL, Solomon, NA. 1981. In Industrial Microbiology and the Advent of Genetic Engineering, pp. 3-14. Scientific American, Freeman &Comp., San Francisco.
• Dharmaraj S. 2010. Marine Streptomyces as a novel source of bioactive substances. World Journal of Microbiology and Biotechnology 26:2123-2139.
• Ertaş M, Özdemir K, Atalan E. 2013. Isolation and characterization of Micromonospora bacteria from various soil samples obtained around Lake Van. African Journal of Biotechnology 12(21):3283-3287.
• Fisher SH, Wray Jr LV. 2002. Bacillus subtilis 168 contains two differentially regulated genes encoding L-asparaginase. Journal of Bacteriology 184:2148-2154
• Goodfellow M, Haynes JA. 1984. Actinomycetes in marine sediments. In Biological biochemical and biomedical aspect of Actinomycetes. Academic Pres, Newyork, 452-472.
• Gulati R, Westhphal JD, Shortell SM. 1997. Customization or Conformity? An Institutional and Network Perspective on the Content and Consequences of TQM Adoption. Administrative Science Quarterly 42:366-394.
• Gupta N, Mishra S, Basak UC. 2007. Occurrence of Streptomyces aurantiacus in mangroves of Bhitarkanika. Malaysian Journal of Microbiology 3:7-14.
• Hakamada Y, Koike K, Yoshimatsu T, Mori H, Kobayashi T, Ito S. 1997. Thermostable alkaline cellulase from an alkaliphilic isolate, Bacillus sp. KSM-S237. Extremophiles, 1:151-156.
• Hamdi YA, Ahmed D, Al-Tai, A.M. 1980. Genera and species of actinomycetes isolated from Iraqi soils. Egyptian Journal of Microbiology 15:7–22.
• Hayakawa M, Yoshida Y, Imura Y. 2004. Selective isolation of bioaktive soil Actinomycetes belonging to the Streptomyces violaceusniger phenotyphic cluster. Journal of Applied Microbiology 96:973-981.
• Hussein AM, Rajab AM, Elgammal AA, Mansour FA, Sami E, Helmy M, Sheheta NE. 1980. Taxonomy of gray pigmented Streptomyces spp. isolated from Egyptian soil. Egyptian Journal of Botany 23:9–16.
• Jones KL. 1949. Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. Journal of Bacteriology 57:141-145.
• Karnetova J, Mateju J, Rezarka T, Prochazka P, Nohynek M, Rokes J. 1984. Estimation of lipsae activity by the diffusion plate method. Folia Microbiologica 29:346-347.
• Langham CD, Williams ST, Sneath PHA, Mortimer AM. 1999. New probability matrices for identification of Streptomyces. Journal of General Microbiology 135:121– 133.
• Narayana KJP, Kumar KG, Vijayalakshmi M. 2008. L-asparaginase production by Streptomyces albidoflavus. Indian Journal of Microbiology 48:331-336.
• Orhan E, 2013. Tuzcul Habitatlardan Alınan Toprak Numunelerinden Halofilik Streptomyces Türlerinin İzolasyonu, Karakterizasyonu ve Teşhisi. Yüksek Lisans Tezi. Van Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü, Van.
• Özdemir K. 2008. Lens orientalis (Boiss.) Hand & Mazz. ve Cicer anatolicum Alef. rizosferinden Streptomyces türlerinin izolasyonu, teşhisi ve karakterizasyonu. Doktora tezi. Yüzüncü Yıl Üniversitesi, Fen Bilimleri Enstitüsü, Van.
• Öztürk E, 2000. Termofilik Streptomyces’lerin İzolasyonu ve Nümerik Taksonomisi. Y.L.Tezi. Ondokuz Mayıs Üniversitesi, Fen-Edebiyat Fakültesi, Biyoloji Bölümü, Samsun, Türkiye.
• Saadoun I, Mohammadi MJ, Al-Momani F, Meqdam M. 1998 Diversity of soil streptomycetes in northern Jordan. Actinomycetes 9:53–58.
• Saddler GS. 1988. Selective isolation and rapid identification of streptomycetes. Ph.D. Thesis, Department of Microbiology, University of Newcastle upon Tyne (UK).
• Seçkin H, 2018. Van Gölüne Dökülen Akarsulardan Streptomyces Türlerinin İzolasyonu Teşhisi ve Moleküler Tanısı. Doktora Tezi. Van Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü, Van.
• Semêdo L, Gomes RC, Linhares AA, Duarte GF, Nascimento RP, Rosado AS, Margis-Pinheiro M, Margis R, Silva K, Alviano CS, Manfio GP, Soares R, Linhares LF, Coelho R. 2004. Streptomyces drozdowiczii sp. nov., a novel cellulolytic streptomycete from soil in Brazil. International Journal of Systematic and Evolutionary Microbiology 54:1323-1328.
• Shirling EB, Gottlieb D. 1966. Methods for characterization of Streptomyces species. International Journal of Systematic Bacteriology 16:313-340.
• Stanton LJ. 1984. Actinomycetes Associated with Freshwater Habitats. PhD thesis, University of Newcastle upon Tyne.
• Vickers JC, Williams ST, Ross GW. 1984. A taxonomic approach to selective isolation of Streptomyces. In Biological, Biochemical and Biomedical Aspects of actinomycetes, pp. 553-561. Edited by L. Ortiz-Ortiz, L. F. Bojalil and V. Yakoleff. Academic Press: Orlando.
• Williams ST, Goodfellow M, Alderson G, Wellington EMH, Sneath PHA, Sackin MJ. 1983a. Numerical classification of Streptomyces and related genera. Journal of General Microbiology 129:1743-1813.
• Williams ST, Goodfellow M, Wellington EMH, Vickers JC, Alderson G, Sneath PHA, Sackin MJ, Mortimer AM. 1983b. A probability matrix for identification of some streptomycetes. Journal of General Microbiology 129:1815- 1830.
• Williams ST, Vickers JC. 1988. Detection of actinomycetes in the natural environment-problems and perspectives. In Biology of Actinomycetes 88, Edited by Y. Okami, T. Beppu ve H. Ogawara, Japan Scientific Societies Pres, Tokyo, 265-270.
• Wiseman A. 1987. Handbook of Enzymes Biotechnology. Second Edition. C 3. The Application of Enzymes in Industry p. 274-373.
• Yin C, Kikuchi K, Hochgreb T, Poss KD, Stainier DY. 2010. Handz Regulates Extracellular Matrix Remodeling Essential for Gut-Looping Morphogenesis in Zebrafish. Developmental Cell 18(6): 973-984