Use of Endophytic Bacteria for the Biological Control of Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)

Yıl 2017, Cilt: 8 Sayı: 2, 107 - 124, 15.12.2017

Pınar Özsarı , Mustafa Akbaba , Hatice Özaktan Yusuf Karsavuran

Öz

Abstract: The main purpose of this study was to investigate the biocontrol potential of 19 strains of endophytic bacteria (EB), and Pseudomonas fluorescens 184 (Pf) which was isolated from diseased Leptinotarsa decemlineata, on larvae of L. decemlineata. First, second, third and fourth-instar larvae were starved for 16-h. They were then placed in petri dishes containing fresh potato leaves and sprayed with the EB suspensions and P. fluorescens 184. Larvae and potato leaves treated with sterile distilled water was used the negative control. The larvae were incubated for 8 days to determine the mean mortality percentage. The highest insecticidal activity, 80% mortality within 8 days, was in larvae treated with EB strain Pantoea agglomerans CC372-83. This test on fresh potato leaves was a quick and reliable screening method for potential biological control agents of L. decemlineata larvae. This work will continue with in vivo pot test.

Anahtar Kelimeler

Biological control, potato beetle, Leptinotarsa decemlineata, Pseudomonas fluorescens

Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)’nın biyolojik mücadelesinde bakteriyel endofitlerin kullanılması

Öz

Bu çalışmanın amacı; laboratuvar stoklarından seçilen 19 endofitik bakteri (EB) izolatının ve daha önce renk değişimi gözlenen Patates böceği larvasından izole edilen bir Pseudomonas fluorescens 184 (Pf) izolatının L. decemlineata larvalarına etkilerinin araştırılmasıdır. Birinci, ikinci, üçüncü ve dördüncü dönem L. decemlineata larvaları 16 saat süreyle aç bırakıldıktan sonra, EB süspansiyonu uygulanmış sağlıklı patates yaprakları bulunan petri kaplarına bırakılmıştır. Negatif kontrol olarak sadece steril su ile uygulama görmüş larva ve patates yaprakları kullanılmıştır. Petri kaplarında 8 gün süreyle tutulan larvaların ortalama ölüm oranı (%) saptanmıştır. Testlenen EB izolatlarından Pantoea agglomerans (Pa) CC372-83larvalarda %80 ölüm oranıyla en başarılı uygulama olmuştur. L. decemlineata larvalarının biyolojik mücadelesi açısından koparılmış yaprak testinin duyarlı ve hızlı bir ön eleme yöntemi olduğu dikkati çekmiştir. Bu çalışma in vivo saksı denemeleri ile devam edecektir.   
 

Anahtar Kelimeler

Biyolojik mücadele, patates böceği, Leptinotarsa decemlineata, Pseudomonas fluorescens, , Pantoea agglomerans

Kaynakça

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