Evaluation of the effect of daily cow's milk production on liver enzyme levels

Year 2024, Volume: 8 Issue: 1, 71 - 79, 01.04.2024

Muhamed Katica , Adıs Mukaca , Alen Salkić , Aida Bešıć , Muamer Obhođaš , Nejra Karaman

https://doi.org/10.47748/tjvr.1372322

Abstract

Objective: The aim of the study was to determine the possible impact of the total daily amount of skim milk on the level of bilirubin and liver enzymes through regression analysis.

Materials and Methods: The study included 63 Holstein-Friesian cows. They were formed in 3 groups, based on the amount of daily milk production. Peripheral blood was punctured, through which the activities of total bilirubin were analyzed (μmol/L), as well as liver enzymes: alanine aminotransferase – ALT (U/L), aspartate aminotransferase – AST (U/L), lactate dehydrogenase – LDH (U/L) and alkaline phosphatase – ALP (U/L).

Results: The lowest concentration of total bilirubin in blood plasma was recorded in the group of cows that have the lowest daily milk production (1.295 ± 0.255 µmol/L), and highest concentration is in cows that produce the most milk (1.855 ± 0.159 µmol/L), but intergroup differences are not significant. Regression analysis found a statistically significant relationship between the amount of produced daily milk and the concentration of total bilirubin (R2=0.132, p=0.005<0.05). It is determined that the highest activity of ALT, AST, ALP and LDH was found in cows with the highest amount of daily skimmed milk, but intergroup differences were not statistically significant (p>0.05).

Conclusion: The activities of bilirubin and liver enzymes in the examined cows were in physiological balance. This indicates that the cows on the farm are raised in modern and good zootechnical and feeding conditions. In such conditions, dairy cows are able to maintain blood composition and homeostatic integrity within physiological limits and adequate reproductive and productive capacity.

Keywords

Liver enzymes, bilirubin, lactating cows, milk

Supporting Institution

Univeristy of Sarajevo - Veterinary Faculty

References

• Akgül G, Şentürk S, Mecitoḡlu Z, Akkaya F. Relationship between some biochemical parameters in healthy and dairy cows with subclinical ketosis. Veterinaria. 2017; 66(2):83-86.
• Aladrović J, Pavković M, Beer-Ljubić B, Vranković L, Stojević Z. Metabolic profile in Holstein dairy cow herd. Vet Stanica. 2018; 49(1):9-18.
• Andjelić B, Djoković R, Cincović M, et al. Relationships between milk and blood biochemical parameters and metabolic status in dairy cows during lactation. Metabolites. 2022; 12(8):733.
• Belic B, Cincovic M, Lakic I, et al. Metabolic status of dairy cows grouped by anabolic and catabolic indicators of metabolic stress in early lactation. Acta Sci Vet. 2018; 46:9.
• Bugarski D. The concentration of insulin, IGF-I and parameters of indicators of the functional state of the liver in the blood of healthy and ketosis cows. (Master's thesis). Belgrade: University of Belgrade, Faculty of Veterinary Medicine, Serbia, 2002.
• Đoković R. Concentration of lipids, triiodothyronine, thyroxine, insulin and cortisol in the blood and histological changes in the liver of cows in the peripartum period. (Doctoral dissertation). Belgrade: University of Belgrade, Faculty of Veterinary Medicine, Serbia, 1998.
• Eşki F, Kurt S. Effect of lactation number on milk yield in Holstein dairy cows. TJVR. 2021; 5 (1):1-4.
• Faixova Z, Kovačević-Filipović M, Božić T. Pathophysiology of the gastrointestinal tract. In: Božić T, ed. Pathological physiology of domestic animals. 2nd ed. Belgrade: Mladost biro d.o.o.; 2012; 491-509.
• Filipejová T, Kováčik J. Evaluation of selected biochemical parameters in blood plasma, urine and milk of dairy cows during the lactation period. Slovak J Anim Sci. 2009; 42, SUPPLEMENT 1:8-12.
• Ghada AE. Mohamed. Investigation of some enzymes level in blood and milk serum in two stages of milk yield dairy cows at assiut city. Assiut Vet Med J. 2014; 60 (142):110-120.
• Hadžimusić N, Krnić J. Enzyme activities of ALT, AST, ALP and LDH in the plasma of cows depending on the reproductive cycle and season. Veterinaria. 2010; 59(1-4),47-57.
• Hadžimusić N. Standardization reference values of biochemical blood parameters of cows in farm breeding. (Master’s thesis). Veterinary Faculty, University of Sarajevo, Bosnia and Herzegovina, 2010.
• Hodžić A, Šaljić E, Crnkić Ć, et al. Metabolic status of the liver of dairy cows of different production under controlled feeding conditions. Veterinaria. 2007; 56(3–4): 99–116.
• IBM Corp. Released. IBM SPSS Statistics for Windows, Version 24.0. Armonk, NY: IBM Corp, 2016.
• Jašović B, Đoković R, Kurćubić V, et al. Assessment of the functional status of liver cells in dairy c ows based on changes in the serum activity of AST, ALT, GGT and LDH. “XXI Savetovanje biotehnologiji”. Zbornik radova, 2016; 21(24):485-489.
• Jovanović MJ, Rajić I, Pešterac V, Črčev D, Čokrevski S. Blood parameters of heavily pregnant and newly calved cows fed with rations of different composition, Veterinarski glasnik. 1997; 51(5-6): 231-244
• Jovanović MJ, Stamatović S, Boroš I, Pivnički Z, Damnjanović Z. Values of some blood parameters in cows suffering from chronic ketosis, endometritis and mastitis. Vet glas. 1991; 10: 697-701.
• Kaneko JJ, Harvey W, Bruss ML. Clinical Biochemistry of Domestic Animals. San Diego, London, Boston, New York, Sydney, Tokyo, Toronto: Academic Press; 2008. p.146-211.
• Katica M, Mukača A, Šaljić E, Čaklovica K. Daily Milk Production in Cows: The Effect on the Concentration Level of Total Cholesterol in Blood Serum. Dairy and Vet Sci J. 2019; 12(4):JDVS.MS.ID.555841.
• Klein R, Nagy O, To´thova´ C, Chovanova F. Clinical and Diagnostic Significance of Lactate Dehydrogenase and Its Isoenzymes in Animals. Vet Med Int. 2020; 5346483 11.
• Krnić J, Podžo M, Hodžić A. Metabolic profile of lactating and peripartum cows. Veterinaria. 2003; 52(1-4), 75-86.
• Lechtenberg KF, Nagaraja TG. Hepatic ultrasonography and blood changes in cattle with experimentally induced hepatic abscesses. Am J Vet Res. 1991; 52(6): 803–891.
• McSherry BJ, Lumsden JH, Valli VE, Baird JD. Hyperbilirubinemia in Sick Cattle. Can J Comp Med. 1984; 48: 237-40.
• Mukača A. Influence of daily milk production on the metabolic profile of cow blood in farm breeding. (Master’s thesis). University of Sarajevo-Veterinary Faculty, Bosnia and Herzegovina, 2015.
• Official Gazette of BꝸH, no. 25/2009 and 9/2018. Available at: https://www.paragraf.ba/propisi/bih/zakon-o-zastiti-i-dobrobiti-zivotinja.html. Accessed May 22, 2022.
• Ogunmoyole T, Fatile OG, Johnson OD, Yusuff AA. Pergularia daemia (Apocynaceae) mitigates rifampicin-induced hepato-renal injury: potentials in the management of liver and kidney diseases. Int J Plant Based Pharm. 2022; 2(2) 196-204.
• Radostits OM, Gay CC, Blood DC, Hinchcliff KW. Veterinary Medicine. 9th ed. London: WB. Saunders; 2000. p. 1819–1822.
• Reid IM, Roberts J. Fatty liver in dairy cows. In Practice. 1982; 4:164-169.
• Reynolds CK, Tyrrel HF, Reynolds PJ. Effects of diet forage-to-concentrate ratio and intakeon energy metabolism in growing beef heifers: netnutritient metabolism by visceral tissues. J Nutrition. 1991; 121: 1004-1015.
• Sako T, Urabe S, Kusaba A, et al. Comparison of Plasma Metabolite Concentrations and Lactate Dehydrogenase Activity in Dogs, Casts, Horses, Cattle and Sheep. Vet Res Communicats. 2007; 31:413-417.
• Sato J, Kanata M, Yasuda J, et al. Changes of serum alkaline phosphatase activity in dry and lactational cows. J Vet Med Sci. 2005; 67(8):813-815.
• Sane RS, Steinmann GG, Huang Q, et al. Mechanisms Underlying Benign and Reversible Unconjugated Hyperbilirubinemia Observed with Faldaprevir Administration in Hepatitis C Virus Patientss. J Pharmacol Exp Ther. 2014; 35: 403-412.
• Stojević Z, Piršljin S, Milinković-Tur S, Zdelak-Tuk M, Ljubič Beer B. AST, ALT and GGT activities in clinically healthy lactating and dry cows. Vet Arhiv. 2005; 75: 67-73.
• Šaljić E, Varatanović M, Ćutuk A, Hrković-Porobija A, Šerić-Haračić S. Evaluation of Liver Transaminases Activities in Lactating Cows in Different Diet Conditions. Veterinaria. 2022; 71(2):233-242.
• Tedesco D, Tava A, Galletti S, et al. Effects of silymarin, a natural hepatoprotector, in periparturient dairy cows. J Dairy Sci. 2004; 87:2239-2247.
• Utari TR, Pudyani PS, Ana ID, Asmara W. The Potential of Bisphosphonate Risedronate Hydrogel In Preventing Relapse Movement, Cumhur Dent J, 2022; 25(2):103-110.
• Valocky I, Legath J, Lenhardt L, Lazar G, Novotny F. Activity of alkaline phosphatase, acidic phosphatase and nonspecific esterase in the oviducts of puerperal ewes after exposure to polychlorinated biphenyls. Veterinarni Medicina. 2007; 52:186-192.
• Yeniyol S, Ricci JL. Alkaline phosphatase levels of murine pre-osteoblastic cells on anodized and annealed titanium surfaces. Eur Oral Res. 2018; 52:12-18.
• Yokus B, Cakir UD. Seasonal and Physiological Variations in Serum Chemistry and Mineral Concentrations in Cattle. Biolog Trace Element Res. 2006; 109:255-266.