TOXIC EFFECTS OF DICHLORVOS ON GALLERIA MELLONELLA LARVAE

Yıl 2017, Cilt: Volume 2 Sayı: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 249 - 249, 16.02.2017

Mustafa Coşkun , Tamer Kayış Osman Dursun İskender Emre

Öz

Organophosphate
insecticides like a Dichlorvos are widely used for the control of agricultural,
industrial, and domestic pests. Nontarget organisms, including humans, are
exposed to toxic concentrations of organophosphates primarily through food,
drinks, and inhalation that can result in neurologic and behavioral disorders,
genotoxic and oxidative damage and loss of ion homeostasis.

This study aims
to determine the physiological, biochemical and toxic effects of DDVP on model
organism, G. mellonella.

Fourth instar of
G. mellonella larvae were fed sublethal DDVP concentrations (2, 4, 6, and 8
mg/100 g feed) until the last instar stage. Protein and MDA content,
antioxidant enzymes activities and ion levels were measured. 

SOD and CAT
activity significantly increased in all DDVP concentrations compared to control
The MDA level was not significantly affected by low DDVP concentrations (2 mg
DDVP/100 g feed) but significantly increased at higher concentrations of DDVP,
respectively 3.292, 7.898, and 10.031 nmol/mg protein. Sodium ion levels
significantly decreased at all DDVP concentrations compared with controls. The
potassium ion level significantly increased in high DDVP concentrations.

Exposure to DDVP
resulted in induction of superoxide dismutase and catalase activities, and lipid
peroxidation in larvae. Potassium and sodium ion levels were significantly
altered by DDVP exposure. These results suggest that DDVP causes oxidative
stress and lipid peroxidation and alters the antioxidant enzyme activities and
ion balance in G. mellonella larvae.

Anahtar Kelimeler

TOXIC EFFECTS OF DICHLORVOS ON GALLERIA MELLONELLA LARVAE

Kaynakça

• Mustafa COŞKUN, Tamer KAYIŞ, Osman DURSUN, Iskender EMRE
• Department of Biology, Faculty of Science -Literature, Adıyaman University, Adiyaman, Turkey Department of Biology, Faculty of Science -Literature, Cukurova University, Adana, Turkey