THE ASSOCATION BETWEEN THIOL DISULPHIDE HOMEOSTASIS AND COBALAMIN

Yıl 2017, Cilt: Volume 2 Sayı: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 254 - 254, 16.02.2017

Murat Alışık , Salim Neşelioğlu Cemil Nural Betül Özbek Özcan Erel

Öz

Vitamin B12
(cobalamin) has a role in protein and DNA synthesis. Cobalamin deficiency
causes many pathologies such as neuropathy and pancytopenia. Thiol groups plays
important roles in many biologic processes including myeloprolipheration, cell
proliferation, formation of tertiary structure of protein, and anti-oxidation
by formation functional or structural disulphide bonds. This study was aimed to
investigate relationship between cobalamin levels and thiol-disulphides
homeostasis.

Test results of
201 patiens who applied at 02.2015-08.2015 have eveluated. It was investigated
the relationship between cobalamin-homocysteine levels and thiol-disulphide
homeostasis. The study was splitted into two groups as low cobalamin with high
homocysteine levels (group 1) and normal cobalamin values with normal
homocysteine values (group 2).

Negative
significant correlation was found between homocysteine levels and native and
total thiol levels (r=-0.263; r=-0.252; p<0.001, respectively). In group 1,
native and total thiol levels was significantly lower than the group 2(p=0.011,
p=0.009, respectively). There was no significance between group 1 and 2 at
disulphide levels, disulphide/native thiol and disulphide/total thiol ratios.

In group 1 there
was no difference in disulphide side while serum native and total thiol levels
was lower than group 2. Results were showed that cobalamin effects thiol side
of the thiol disulphide homeostasis. It thought that decrease of thiol levels
based on decreased protein synthesis in cobalamin deficiency, despite increased
homocysteine levels in cobalamin deficiency. Decreased serum thiol level causes
decreasing antioxidane capacity and it may be a point at the neurodegeneration
in cobalamine deficiency. This results suggest that thiol sources replacement
with cobalamine may be beneficial.

Anahtar Kelimeler

THE ASSOCATION BETWEEN THIOL DISULPHIDE HOMEOSTASIS AND COBALAMIN

Kaynakça

• Murat ALIŞIK, Salim NEŞELİOĞLU, Cemil NURAL, Betül ÖZBEK, Özcan EREL
• Clinical Biochemistry, Yıldırım Beyazıt University, Ataturk Training and Research Hospital, Ankara, Turkey