Asphodelus ramosus, Allium cepa L. ve Allium porrum L. Bitkilerinin Antioksidan Kapasitesi ve Fenolik Bileşenlerin HPLC ile Ayrımı

Year 2017, Volume: 4 Issue: 4, 499 - 505, 26.10.2017

Elif Apaydın , Gülnur Arabacı

Abstract

Sarızambak
olarak bilinen ve latince adı Asphodelus
Ramosus olan, çiriş bitkisinin antioksidan özellikleri incelenmiştir. Bu
amaçla metanol, % 50 metanol, etil asetat ve su ekstraktları ile çalışılmıştır.
Bitki iki farklı sıcaklıkta kurutulup 
(35 ºC ve 65 ºC ), böylece sıcaklık farklılığının bir parametre
oluşturması sağlanmıştır. Ekstraksiyon işleminden sonra tüm ekstraktların
toplam fenolik bileşenlerine Folin Ciocalteu yöntemi ve toplam
flavonoid bileşenlerine aluminum nitrat
metodu uygulanmıştır. Buna ilaveten DPPH serbest radikal
giderici etkisi, H₂O₂ süpürücü etkisi, bakır (II) iyonu indirgeme
gücü (CUPRAC), süperoksit anyon
radikali süpürme kapasitesi analiz edilmiş ve tüm bunlar HPLC (Yüksek
Performanslı Sıvı Kromatografi) ile desteklenmiştir.

Keywords

Asphodelus ramosus, ekstraksiyon, flavonoid, DPPH, H2O2, CUPRAC, HPLC

Antioxidant Capacity and Phenolic Compounds with HPLC of Asphodelus ramosus and Comparison of the Results with Allium cepa L. and Allium porrum L. Extracts

Abstract

Asphodelus ramosus
known as a yellow lily which has been a plant of onion for this reason, this
plant was studied with about antioxidant activity of methanol, 50 % methanol,
ethyl acetate and water extracts. The plant was dried at two different
temperatures (35 ºC
and 65 ºC)
so as to understand how to work the difference in temperature effects. After
extraction, all solvent extracts were studied for their total phenolic contents
using Folin Cioceltau reagent, total
flavonoid content with aluminum nitrate method. In addition, DPPH free redical scavenging through
the determination, the determination of the activity of
removal of H₂O₂,
copper (II) ion reducing antioxidant capacity
determination (CUPRAC), removal activity of super oxide anion were studied and the results were supported with HPLC
(High Performance Liquid Chromatography).

Keywords

Asphodelus ramosus, extraction, flavonoid, DPPH, H2O2, CUPRAC, HPLC

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