Antifungal Activity of Thymbra spicata L. and Rosmarinus officinalis L. Essential Oils against Monilinia fructigena Honey in Whetze

Year 2018, Volume: 5 Issue: 2, 121 - 126, 06.05.2018

Melih Yılar , Yusuf Bayar

https://doi.org/10.30910/turkjans.421344

Cited By: 1

Abstract

This study was carried out to
determine the antifungal potential of Thymbra
spicata L. and Rosmarinus officinalis
L. (Lamiaceae) plant essential oils against the Monilinia fructigena Honey in Whetze pathogen which is one of the
major disease agents of soft seeds. The essential oils were dropped on a filter
paper adhered to the covers of petri dishes with a micropipette at the doses of
0 (Control), 0.5, 0.8, 1, 1.5, 2, 4, 8, 16 μl/petri dish. At the end of the
seven days incubation period, the diameters of the mycelium growth in petri
dishes were measured with an automatic caliper. As a result, T. spicata L. and R. officinalis plant essential oils were found to be statistically
significant on the pathogen. Depending on the increased dose, R. officinalis essential oil inhibited
the development of the M. fructigena mycelium and inhibited by 100% in 16 μl/petri dish. On the other hand, T. spicata essential oil influenced the mycelium development of the
pathogen more and inhibited it by 100% at the dose of 2 μl/petri
dish. As a result of dose-response study regarding T.
spicata and R. officinalis essential oils against M. fructigena  the LC₅₀
and LC₉₀ values were found to be 0.80-1.63 and 5.36-12.17,
respectively. These findings showed that T. spicata and R. officinalis essential oils may be an alternative to
synthetic fungicides for controlling M.
fructigena.

Keywords

Thymbra spicata, Rosmarinus officinalis, Monilinia fructigena, essential oil, antifungal activity, biopescticide

Thymbra spicata L. ve Rosmarinus officinalis L. Uçucu Yağlarının Monilinia fructigena Honey in Whetze’ya Karşı Antifungal Aktivitesi

Abstract

Bu
çalışma, yumuşak çekirdeklilerin önemli hastalık etmenlerden biri olan Monilinia
fructigena patojenine
karşı Thymbra spicata L. ve Rosmarinus officinalis L. (Lamiaceae)
bitki uçucu yağlarının antifungal potansiyelinin belirlenmesi amacıyla
yürütülmüştür. Uçucu yağlar petri kaplarının kapaklarına yapıştırılan filtre
kağıdına 0 (Kontrol),0.5, 0.8, 1, 1.5, 2, 4, 8, 16 μl/petri dozda mikropipetle
damlatılmıştır.  Yedi günlük inkubasyon
süresi sonunda petrilerdeki miselyum çapları otomatik kumpas ile ölçülmüştür.
Sonuçta T. spicata L. ve R. officinalis bitki uçucu yağları
patojen üzerine istatistiki olarak önemli derece etkili bulunmuştur. R. officinalis uçucu yağı artan doza
bağlı olarak M. fructigena miselyum
gelişimini engellemiş olup 16 μl/petri kabı dozda %100 engellemiştir. T. spicata uçucu yağı ise patojenin
miselyum gelişimi üzerine daha fazla etki göstermiş; 2 μl/petri
kabı dozda %100 inhibe etmiştir. T.
spicata ve R. officinalis uçucu yağlarının M. fructigena karşı
doz-etki çalışması sonucunda LC₅₀ ve LC₉₀ değerleri
sırasıyla, 0.80-1.63 ve 5.36-12.17 olarak bulunmuştur. Bu bulgular, T. spicata ve R. officinalis uçucu yağlarının,  M.
fructigena kontrolünde sentetik fungusitlere alternatif olabileceğini
göstermiştir.

Keywords

Thymbra spicata, Rosmarinus officinalis, Monilinia fructigena, uçucu yağ, antifungal aktivite, biyopestisit

References

• Anonymous, 2017. Elma Monilinia fructigena özellikleri. http://www.bitkisagligi.net. URL (Date of Access: 15.10.2017).
• Arslan, Ü., Karabulut, Ö.A. 2005. Baharat bitkilerinin bitki patojeni funguslara karşı antifungal etkisi. Atatürk University Journal of the Faculty of Agriculture, 36(2): 131-135.
• Bagamboula, C.F., Uyttendaele, M., Debevere, J. 2004. Inhibitory effect of thyme and basil essential oils, carvacrol, thymol, estragol, linalool and p-cymene towards Shigella sonnei and S. flexneri. Food Microbiology, 21: 33-42.
• Barakat, A., Wakim, L.H., Apostolides, N.A., Srour, G., Beyrouthy, M.E. 2013. Variation in the essential oils of Thymbra spicata L. growing wild in Lebanon according to the date of harvest. The Journal of Essential Oil Research, 25(6): 506-511.
• Belgüzar, S., Yanar, Y., Taş, L., Kadıoğlu, İ., Yılar, M. 2013. Determination of antifungal activities of Origanum minutiflorum (Thyme) extract and essential oil. I. Plant Protection Products and Machines Congress. April 2 to 5, Antalya, pp. 207-215.
• Cakir, A., Kordali, S., Kilic, H., Kaya, E. 2005. Antifungal properties of essential oils and crude extracts of Hypericum linarioides. Biochemical Systematics and Ecology, 33: 245-256.
• Carvalho, G.S., Silva, L.B., Silva, L.S., Almeida, M.L.S., Carneiro, E., Cândido, A.C.S., Peres, M.T.L.P. 2016. Insecticidal activity of plant extracts and essential oils of bleed water against the bean weevil. Journal of Stored Products and Postharvest Research, 7(7): 69-75.
• Douiri, L.F., Boughdad, A., Alaoui, M.H., Moumni, M. 2014. Biological activity of Rosmarinus officinalis essential oils against Callosobruchus maculatus, (Coleoptera, Bruchinae). Journal of Biology, Agriculture and Healthcare, 4(2): 5-14.
• Edris, A.E., Farrag, E.S. 2003. Antifungal activity of peppermint and sweet basil essential oils and their major aroma constituents on some plant pathogenic fungi from the vapor phase. Food/Nahrung, 47: 117-121.
• Hassannejad, S., Ghafarbi S.P. 2013. Allelopathic effects of some Lamiaceae on seed germination and seedling growth of dodder (Cuscuta campestris Yunck.). International Journal of Biosciences, 3(3): 9-14.
• Işık, D., Mennan, H., Cam, M., Tursun, N., Arslan, M. 2016. Allelopathic potential of some essential oil bearing plant extracts on Common Lambsquarters (Chenopodium album L.). Rewista de Chimie(Bucharest), 67(3): 455-459
• Kadıoğlu, İ, Yanar, Y., Hasgül, Y., Yılar, M., Belgüzar, S., Koyuncu, E. 2013. Determination of antifungal activities of some Thyme species. I. Plant Protection Products and Machines Congress. April 2 to 5, Antalya, pp. 217-226.
• Kaur, S., Singh, H.P., Mittal, S., Batish, D.R., Kohli, R.K. 2010. Phytotoxic effects of volatile oil from Artemisia scoparia against weeds and its possible use as a bioherbicide. Industrial Crops and Products, 32:54-61.
• Ksouri, S, Djebir, A., Bentorki, A.A., Gouri, A., Hadef, Y., Benakhla, A. 2017. Antifungal activity of essential oils extract from Origanum floribundum Munby, Rosmarinus officinalis L. and Thymus ciliatus Desf. against Rosmarinus officinalis L. Candida albicans isolated from bovine clinical mastitis. Journal de Mycologie Médicale, 27(2): 245-249.
• Lopez-Reyes, J.G., Spadaro, D., Gullino, M.L., Garibaldi, A. 2010. Efficacy of plant essential oils on postharvest control of rot caused by fungi on four cultivars of apples in vivo. Flavour Fragrance J. 25: 171-177.
• Mahilrajan, S., Nandakumar, J.,Kailayalingam, R., Manoharan, N.A., SriVijeindran, S. 2014. Screening the antifungal activity of essential oils against decay fungi from palmyrah leaf handicrafts. Biological Research, 47(1): 35.
• Mancini, E., Camele, I., Elshafie, H.S., Martino, L., Pellegrino, C., Grulova, D., Feo, V. 2014. Chemical composition and biological activity of the essential oil of Origanum vulgare ssp. hirtum from different areas in the Southern Apennines (Italy). Chemistry & Biodiversity, 11: 639-651.
• Matsuzaki, Y., Tsujisawa, T., Nishihara, T., Nakamura, M., Kakinoki, Y. 2013. Antifungal activity of chemotype essential oils from rosemary against Candida albican. Open Journal of Stomatology, 3: 176-182.
• Pandey, D.K., N.N. Tripathi, R.D. Tripathi,, S.N., Dixit, 1982. Fungitoxic and phytotoxic properties of essential oil of Hyptis suaandolens. Z. Pflanzenkrankheiten Pflanzenschutz, 89: 344-349.
• Panizi, L., Flamini, G., Cioni, P.L., Morelli, I. 1993. Composition and antimicrobial properties of essential oils of four Mediterranean Lamiaceae. Journal of Ethnopharmacology, 39(3): 167-170.
• Soylu, E.M., Yiğitbas, H., Tok, F.M., Soylu, S., Kurt, S., Baysal, O., Kaya, A.D. 2005. Chemical composition and antifungal activity of the essential oil of Artemisia annua L. against foliar and soil-borne fungal pathogens. Zeitschrift fu¨r Pflanzenkrankheiten und Pflanzenschutz, 112: 229-239.
• Soylu, E.M., Kurt, S., Soylu, S. 2010. In vitro and in vivo antifungal activities of the essential oils of various plants against tomato grey mould disease agent Botrytis cinerea. Int.J. Food Microbiol. 143: 183-189.
• Ünlü, M., Vardar-Ünlü, G., Vural, N., Dönmez, E., Özbaş, Z.Y. 2009. Chemical composition, antibacterial and antifungal activity of the essential oil of Thymbra spicata L. from Turkey. Natural Product Research, 23(6): 572-579.