Zeytin (Olea europaea) yaprağı ekstraktlarının model organizma Galleria mellonella hemosit ve hemosit aracılı bağışıklık tepkileri üzerine etkileri

Yıl 2020, Cilt: 7 Sayı: 3, 646 - 653, 20.07.2020

Serhat Kaya , Neslihan Demir

https://doi.org/10.30910/turkjans.691138

Cited By: 7

Öz

Zeytin ağacı (Olea europaea) ülkemizin de içerisinde yer aldığı Akdeniz bölgesinde yaygın olarak yetiştirilen bir türdür. Bu ağacın meyvesi ve yağından yararlanıldığı kadar, yapraklarından da halk hekimliğinde yararlanılmaktadır. Antioksidan, anti-enflamatuar ve antibiotik etkilerinin yanında kalp hastalıklarından korunma, karaciğer yağlanması, diabet gibi bazı yaygın hastalıklara karşı da etkili olduğu bilinmektedir. Zeytin yaprağının en önemli bileşeni oleuropeindir. Çalışmamızda; insan patojenleri için sıklıkla kullanılan model organizma Galleria mellonella (Büyük Balmumu Güvesi) kullanılmıştır. Zeytin yaprağı ekstraktı ile muamele edilen G. mellonella larvalarının toplam hemosit sayısı, enkapsülasyon ve melanizasyon bağışıklık tepkileri üzerine etkileri incelenmiştir. Toplam hemosit sayısının tespiti ve enkapsülasyon-melanizasyon tepkilerinin belirlenmesi için faz-kontrast mikroskop kullanılmıştır. Bulgularımıza göre 1000 ppm dozunda zeytin yaprağı ekstraktı bağışıklığı desteklemektedir.

Anahtar Kelimeler

Zeytin yaprağı ekstraktı, Oleuropein, Galleria mellonella, hemosit, enkapsülasyon, melanizasyon

Destekleyen Kurum

Çanakkale Onsekiz Mart Üniversitesi, Bilimsel Araştırma Projeleri Koordinasyon Birimi

Proje Numarası

FHD-2018-2685

Teşekkür

Bu çalışma, Çanakkale Onsekiz Mart Üniversitesi, Bilimsel Araştırma Projeleri Koordinasyon Birimince (BAP) FHD-2018-2685 kodlu proje kapsamında desteklenmiştir.

Kaynakça

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