Effects of Some Plant Growth Regulators and Phloroglucinol on Micropropagation of SP-2 (Prunus spinosa) Clonal Rootstock

Yıl 2020, Cilt: 7 Sayı: 4, 1057 - 1064, 20.10.2020

Esra Bulunuz Palaz

https://doi.org/10.30910/turkjans.678171

Cited By: 1

Öz

Bu çalışma, 2017 yılında bazı oksin, sitokinin ve phloroglucinol konsantrasyonlarının in vitro kültür koşullarında SP-2(Prunus spinosa) klon anacının rejenerasyon ve köklenme olasılıkları üzerine etkilerini araştırmak amacıyla yürütülmüştür. Materyal olarak ½ MS besin ortamı ve farklı IBA kombinasyonları (0.1-0.5-1.00 mg L-1), NAA (0.25 mg L-1), BA (0.5-1.00-2.00 mg L-1), Kinetin (0.5-1.00-2.00 mg L-1), Phloroglucinol (30-60-90-120 mg L-1) ve MSK-0 (herhangi bir hormon içermeyen normal ½ MS) kullanıldı. Çalışmanın sonunda eksplant başına maksimum sürgün uzunluğunun MSK-7 kombinasyonunda (25.11 mm) olduğu tespit edildi. Ayrıca, en yüksek kök uzunluğu MSK-2 ( 176.74 mm), en yüksek kök sayısı MSK-5 (2 adet/explant) kombinasyonlarında gözlendi. Bu çalışma sonucunda kontrol kombinasyonu (MSK-0) dağılımın alt kısımlarında kalırken, en iyi köklenme değeri MSK-2 ve MSK-3 kombinasyonlarının kullanıldığı bitkilerde görülmüştür. Bu çalışmanın sonucuna göre, Phloroglucinol (PG), klonal anaç adayları SP-2'nin (Prunus spinosa) in vitro çoğaltımında olumlu bir etkiye sahip olduğu belirlenmiştir.

Anahtar Kelimeler

In vitro, Prunus spinosa, mikroüretim, erik, anaç

Effects of Some Plant Growth Regulators and Phloroglucinol on Micropropagation of SP-2 (Prunus spinosa) Clonal Rootstock

Öz

This study was conducted in 2017 in order to work on the impacts of certain auxin, cytokine and phloroglucinol concentrations on propagation and rooting possibilities of SP-2 (Prunus spinosa) in vitro culture conditions. ½ MS nutrient medium and different combinations of IBA (0.1-0.5-1.00 mg L-1), NAA (0.25 mg L-1), BA (0.5-1.00-2.00 mg L-1), Kinetin (0.5-1.00-2.00 mg L-1), Phloroglucinol (30-60-90-120 mg L-1) and MSK-0 (normal ½ MS without any hormone) were used as materials. It was observed at the end of the study that maximum shoot length per explant was at the MSK-7 combination (25.11 mm) and the highest plant multiplication was found as 6 for MSK-10 combinations per explant. In addition, the highest root length was 176.74 mm for the MSK-2 combinations while the highest root number was observed as 2 as per explant for MSK-5 combinations. As a result of this study, while control medium (MSK-0) remained below the distribution, the best rooting value was seen on the material, where MSK-2 and MSK-3 combinations was used. According to the result of this study, Phloroglucinol (PG) had a positive impact in vitro propagation of clonal rootstock candidates SP-2 (P. spinosa).

Anahtar Kelimeler

In vitro, micropropagation, plum, Prunus spinosa, rootstock

Kaynakça

• Ahn, G., Kim, K., Cha, S., Song, C., Lee, J., Heo, M., Yeo, I., Lee, N., Jee, Y., Kim, J., Heu, M., Jeon, Y. 2007. Antioxidant activities of phlorotannins purified from Ecklonia cava on free radical scavenging using ESR and H2O2-mediated DNA damage. European Food Research Technology, 226: 71-79. DOI 10.1007/s00217-006-0510-y.
• Andreu, P., Marin, J. A. 2005. In vitro culture establishment and multiplication of the Prunus rootstock ‘Adesoto101’ (Prunus institia L.) as affected by the type of propagation of the donor plant and by the culture medium composition. Scientia Horticulturae, 106: 258-267. DOİ 10.1016/j.scienta.2005.03.008.
• Armitage, P., Herbert, D. A. 1996. Advances in Biometry. New York, USA, p. 268-275.
• Athanasas, K., Magiatis, P., Fokialakis, N., Skaltsounis, A.L., Pratsinis, H., Kletsas, D. 2004. Hyperjovinols A and B: two new phloroglucinol derivatives from Hypericum jovis with antioxidant activity in cell cultures. Journal of Natural Products, 67: 973-977. DOİ 10.1021/np034051w.
• Crockett, S., Wenzig, E., Kunert, O., Bauer, R. 2008. Anti-inflammatory phloroglucinol derivatives from Hypericum empetrifolium. Phytochemistry Letters, 1: 37-43. DOİ 10.1016/j.phytol.2007.12.003.
• Dimassi-Theriou, K. 1995. In vitro Rooting of Rootstock GF-677 (Prunus amygdalus × P. persica) as influenced by mineral concentration of the nutrient medium and type of culture-tube sealing material. Journal of Horticultural Science, 70: 105-108. DOİ 10.1080/14620316.1995.11515279.
• Fotopoulos, S., Sotiropoulos, T. E. 2005. In vitro Propagation of the PR 204/84 (Prunus persica × P. amygdalus) Rootstock: Axillary shoot production and rhizogenesis. New Zealand Journal of Crop and Horticultural Science, 33: 75-79. DOİ 10.1080/01140671.2005.9514333.
• Gurkan, B., Ugur, R., Gurkan, T. 2017. Reactions of Meloidogyne incognita race 1 and Meloidogyne javanica race 1 against some plum rootstock. Turkish Journal of Agriculture and Natural Sciences, 5: 64-70.
• Jaime, A., Silva, T., Dobranszki, J., Ross, S. 2013. Phloroglucinol in plant tissue culture. In Vitro Cellular and Developmental Biology – Plant, 49: 1-16. DOI 10.1007/s11627-013-9491-2.
• Kong, C., Kim, J., Yoon, N., Kim, S. 2009. Induction of apoptosis by phloroglucinol derivative from Ecklonia cava. Food Chemical Toxicology, 47: 1653-1658. DOİ 10.1016/j.fct.2009.04.013.
• Maas, F. M., Balkhoven-Baart, J., Van der Steeg, P. A. H. 2014. Selection of Prunus spinosa as a dwarfing rootstock for high density plum orchards. Acta Horticulturae, 1058: 507-516. DOİ 10.17660/ActaHortic.2014.1058.63.
• Milosevic, T. 2006. Effects of interstock on seasonal changes in microelement concentrations in apricot leaf. Acta Horticulturae, 701: 719-722. DOİ 10.17660/ActaHortic.2006.701.128.
• Murashige T., Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiology of Plant, 15: 473-497.
• Nas, M. N., Bolek, Y., Sevgin, N. 2010. The effect of explant and cytokine type on regeneration of Prunus microcarpa. Scientia Horticulturae, 126: 88-94. DOİ 10.1016/j.scienta.2010.06.012.
• Odabas, M. S., Cirak, C. 2011. Hypericum. Medical Aromatic Plant Science Biotechnology, 5: 1-107.
• Ozbek, B., Kayım, M., Elekçioğlu, İ. H. 2014. Evaluation of resistance for root-knot nematodes (Meloidogyne incognita and Meloidogyne javanica [Tylenchida: Meloidogynidae]) of some Prunus rootstocks grown in vitro conditions. Journal of Agricultural Faculty of Uludag University, 28(2): 27-35.
• Perez-Tornero, O., Egea, J., Vanoostende, A., Burgos, L. 2000. Assessment of factors affecting adventitious shoot regeneration from in vitro cultured leaves of apricot. Plant Science, 158: 61-70. DOİ 10.1016/S0168-9452(00)00303-4.
• Petri, C., Scorza, R. 2009. Factors affecting adventitious regeneration from in vitro leaf explant of ‘Improved French’ plum, the most important dried plum cultivar in the USA. Annals of Applied Biology, 156: 79-89. Doi:10.1111/j.1744-7348.2009.00364.x.
• Saddique, Z., Naeem, I., Maimoona, A. 2010. A review of the antibacterial activity of Hypericum perforatum L. Journal of Ethnopharmacology, 131: 511-521. DOİ 10.1016/j.jep.2010.07.034.
• Sadeghi, F., Yadollahi, A., Jafarkhani Kermani, M., Eftekhari, M. 2015. Optimizing culture media for in vitro proliferation and rooting of Tetra (Prunus empyrean 3) rootstock. Journal of Genetic Engineering and Biotechnology, 13: 19-23. DOİ.10.1016/j.jgeb.2014.12.006.
• Silveira, C., Fortes, G., Fachinello, J., Rodrigues, A., Citadin, I., Quezada, A., Silva, J. 2002. In vitro multiplication of prunus rootstocks under low concentration and different auxin. Revista Brasilera Fruticulturae, 24: 608-610. DOİ 10.1590/S0100-29452002000300006.
• Stein, A. C., Viana, A. F., Muller, L. G., Nunes, J. M., Stolz, E. D., do Rego, J. C., Costentin, J., Von Poser, G. L., Ratesa, S. M. K. 2012. Uliginosin B, Phloroglucinol derivative from Hypericum polyanthemum: A promising new molecular pattern for the development of antidepressant drugs. Behavioral Brain Research, 228: 66-73. DOİ 10.1016/j.bbr.2011.11.031.
• Ugur, R. 2017. Investigation of Rootstock Characteristics of Some Wild Plum Species Obtained by Clone Selection from Kahramanmaras Flora. Doctoral Dissertation. Cukurova University, Department of Horticulture, p. 324.
• Ugur, R., Paydas Kargi, S. 2017. Investigation of growing situations of apricot varieties grafted on clonal rootstock candidate some wild plum genotypes obtained by selection. Turkish Journal of Natural and Agricultural Science, 5: 64-70.
• Ugur, R., Altun, O., Ozatar, H.O., Aras, S., Erayman, M., Paydas, Kargi, S. 2016. Investigation of rooting possibilities of different Prunus domestica hardwood cuttings obtained by selection. Journal of Ataturk Central Horticultural Research Institute, 45(1): 325-328.
• Winkelmann, K., San, M., Kypriotakis, Z., Skaltsa, H., Bosilij, B., Heilmann, J. 2003. Antibacterial and cytotoxic activity of prenylated bicyclic acylphloroglucinol derivatives from Hypericum amblycalyx. Zeitschrift für Naturforsch Journal of Bioscience, 58: 527-532.
• Vujovic, Ruzic, Dj, Cerovic, R. 2012. In vitro shoot multiplication as influenced by repeated subculturing of shoots of contemporary fruit rootstocks. Hort. Science, 39: 101-107.
• Zou, Y. N. 2010. Micropropagation of Chinese plum (Prunus salicina L.) using mature stem segments. Notulae Botanicae Horti Agrobotanici Cluj, 38(3): 214-218. DOİ 10.15835/nbha3834614.