Formalin ile İnaktive Flavobacterium psychrophilum’un Gökkuşağı Alabalığı (Oncorhynchus mykkiss)’nın Antioksidan Enzimlerine Etkisi

Year 2021, Volume: 8 Issue: 2, 372 - 380, 23.04.2021

Muammer Kırıcı , Mahinur Kırıcı , Mehmet Reşit Taysı , Ünal İspir

https://doi.org/10.30910/turkjans.843023

Abstract

Bu çalışmada, formalin ile inaktive edilen Flavobacterium psychrophilum’un yavru gökkuşağı alabalıklarında bazı antioksidan enzim parametrelerinde oluşturduğu değişiklikler incelenmiştir. Yaklaşık 1.0g ağırlığında olan gökkuşağı alabalığı yavruları formalin ile inaktive edilmiş 1x108 CFU/ml bakterin ile 30, 60 ve 90 dakika muamele edildi. Balıklar, uygulamadan 21 gün sonra 1x 108 CFU/ml canlı F. psychrophilum ile eprüvasyon yapıldı. On dört gün sonra ise balıklardan alınan örnekler ile malondialdehit (MDA), süperoksit dismutaz, katalaz ve redükte glutatyon aktivitelerindeki değişiklikler incelendi. MDA düzeyinde özelikle 60 ve 90 dak. bakteri uygulanan balıklarda ılımlı pozitif kontrol grubuna göre bir azalış gözlendi. Antioksidan enzim aktivitelerinde de aynı şekilde olumlu yönde bir değişiklik saptandı. Sonuç olarak; formalin ile inaktive edilen bakterin preperatının, canlı F. psychrophilum bakterisi tarafından bozulan oksidan/antioksidan dengesinin düzenlenmesinde etkili olabileceği tespit edilmiştir.

Keywords

Flavobacterium psychrophilum, formalin, gökkuşağı alabalığı, oksidan/antioksidan dengesi

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