Kuraklık Stresi Altında Borago officinalis L.'de Antioksidan Savunma Sistemi

Year 2021, Volume: 8 Issue: 4, 1048 - 1055, 24.10.2021

Hülya Torun , Engin Eroğlu

https://doi.org/10.30910/turkjans.949626

Cited By: 2

Abstract

Kuraklık, bitki büyümesini ve gelişmesini sınırlayan temel abiyotik stres faktörlerinden biridir. Bu çalışmada, kuraklık stresinin Borago officinalis (hodan) bitkisi üzerindeki fizyolojik ve antioksidan tepkileri açısından etkileri değerlendirilmiştir. Bu amaç doğrultusunda, bağıl büyüme oranı (RGR), yaprak bağıl su içeriği (RWC), ozmotik potansiyel, klorofil floresansı (Fv/Fm), lipid peroksidasyonu, hidrojen peroksit (H2O2) düzeyi, süperoksit dismutaz (SOD), peroksidaz (POX) , katalaz (CAT), askorbat peroksidaz (APX) ve glutatyon redüktaz (GR) aktiviteleri kontrollü bir serada kuraklık uygulaması altında belirlendi. RGR, RWC ve ozmotik potansiyel önemli ölçüde azalırken, tiyobarbutirik asit reaktif maddeleri (TBARS) olarak ifade edilen lipid peroksidasyonu ve H2O2 içerikleri kuraklık stresi altında arttı. Diğer taraftan, POX, CAT, APX ve GR aktivitelerindeki önemli artışlar lipid peroksidasyonundaki artışı engelleyemedi. Bildiğimiz kadarıyla bu çalışma, Borago officinalis'in enzimatik antioksidan savunma sistemi üzerine kuraklık stresi altında yapılan ilk çalışmadır.

Keywords

Antioxidant enzimler, hodan, kuraklık, oksidatif stres, reaktif oksijen türleri

Antioxidant Defense System in Borago officinalis L. under Drought Stress

Abstract

Drought is one of the main abiotic stress factor that limits plant growth and development. Drought stress effects on Borago officinalis L. (borage) plants in terms of physiological and antioxidant responses have been evaluated in this study. In parallel with this purpose, relative growth rate (RGR), leaf relative water content (RWC), osmotic potential, chlorophyll fluorescence (Fv/Fm), lipid peroxidation, hydrogen peroxide (H2O2) level, superoxide dismutase (SOD), peroxidase (POX), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) activities were determined under drought treatment in a controlled greenhouse. RGR, RWC and osmotic potential were significantly decreased, while lipid peroxidation expressed thiobarbutiric acid reactive substances (TBARS) and H2O2 contents were increased under drought stress. On the other hand, significant increases in POX, CAT, APX and GR activities did not prevent the increase in lipid peroxidation. To the best of our knowledge, this is the first study conducted on the enzymatic antioxidants of the defense system of Borago officinalis L. under drought stress.

Keywords

Antioxidant enzymes, borage, drought, oxidative stress, reactive oxygen species

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