Fenol'e Maruz Kalan Sazan (Cyprinus carpio)’ların Periferik Kan Eritrositlerindeki Anormalliklerin İncelenmesi

Year 2022, Volume: 9 Issue: 3, 518 - 523, 23.07.2022

Mikail Özcan , Engin Şeker , Ünal İspir

https://doi.org/10.30910/turkjans.1092252

Abstract

Fenol ve fenolik bileşikler, hayvanlarda ciddi sıkıntılara neden olan çevresel ksenobiyotiklerdir. Fenol çevrede antropojenik etkiye sahip olup önemli problemlere neden olmaktadır. Bu çalışma, fenolün sazan (Cyprinus carpio) eritrositlerinde olası genotoksik etkilerinin araştırılması amacıyla yapılmıştır. Çalışmada ortalama ağırlıkları 0.474 ± 0.04 g olan balıklar kullanılmıştır. Balıklar 24, 48, 72, 96 saatler boyunca 0 (kontrol), 5, 10 ve 20 ppm fenol ile muamele edilmiştir. Eritrositlerdeki mikronukleus (MN) ve nükleer anormallik (NAs) oluşumu incelenmiştir. Eritrositlerde mikronukleus ve diğer anormalliklerin oluşumunda, fenol konsantrasyonuna bağlı olarak bir artışın olduğu görülmüştür. Mikronükleus testindeki sonuçlar, fenolün güçlü mutajenik bir etkiye sahip olduğunu göstermiştir (p<0.05). Fenol tatlı su ekosistemlerinde yüksek risk taşıyan bir kirletici olduğundan, akuatik ortamların çevresel risk değerlendirmesinin saptanması için daha ayrıntılı çalışmalar yapılmalıdır.

Keywords

Sazan, Cyprinus carpio, Fenol, Mikronukleus, Nükleer Anormal

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