Zebularin'in Antioksidan Enzimleri ve Galleria mellonella (Lepidoptera: Pyralidae) Lipit Peroksidasyonu Üzerindeki Etkisi

Year 2023, Volume: 10 Issue: 2, 457 - 464, 25.04.2023

Ebru Çakır , Fevzi Uçkan , Ekrem Ergin , Rabia Özbek

https://doi.org/10.30910/turkjans.1110745

Abstract

Sitozin metilasyonu'daki sitozin kalıntısını 5-metilsitozine dönüştürmek için bir metil grubu ekleyen süreçtir. Metilasyon sürecine DNA metiltransferazları tarafından aracılık edilir. Zebularin (ZEB), ur ve kist tümör hücrelerinde hipermetile edilmiş genleri yeniden aktive eder, kanser hücreleri üzerinde tercihli bir etki gösteren DNA metiltransferazları ile kovalent bir kompleks oluşturan sitidin analoğudur. Bal mumu güvesi Galleria mellonella’da son evre larvalarına farklı dozlarda (0.25-32 mg / ml) verilen ZEB’in antioksidan enzim aktivitesi ve lipit peroksidasyonu üzerindeki etkileri incelendi. ZEB enjeksiyonu, son aşamada G. mellonella larvalarında CAT enzim aktivitesinde değil ama büyüklük sırasına göre SOD, MDA ve GST seviyesinde artışa neden oldu. Sonuçlar için iki olası sebep öngörülmektedir. Birincisi, ZEB’in, G. mellonella'da DNA metilasyonu üzerindeki engelleyici etkisinin bir sonucu olabilir, bu da böceklerde gen transkripsiyonunun azaltılmasına yol açabilmektedir. İkincisi, yüksek oksidatif stresin enzimlerin azalan aktivitesine neden olması muhtemeldir.

Keywords

antioksidan enzimleri, DNA metilasyonu, Galleria mellonella, zebularin, Lipit peroksidasyonu

Supporting Institution

Kocaeli Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

2015-215Z079

Influence of Zebularin on Antioxidant Enzymes and Lipid Peroxidation of Galleria mellonella (Lepidoptera: Pyralidae)

Abstract

Cytosine methylation is a process that adds a methyl group to a cytosine residue of DNA to convert it to 5-methylcytosine. Zebularine (ZEB) reactivates hypermethylated genes in yeast and solid tumor cells, with DNA methyltransferases that show a preferential effect on cancer cells. We investigated the effects of various doses (0.25-32 mg/ml) of ZEB, on antioxidant enzyme activity and lipid peroxidation in wax moth Galleria mellonella last instars. Injection of ZEB in last instars G. mellonella resulted in increases in SOD, MDA, and GST levels, in order of magnitude, but not in CAT enzyme activity. Two possible reasons were predicted for these results. This effect could be a result of the inhibitory effect of ZEB on DNA methylation in G. mellonella, which leaded to reducing gene transcription in insects. Or it was likely that high oxidative stress might cause a decreasing activity of enzymes.

Keywords

Antioxidant enzymes, DNA methilation, Galleria mellonella, lipid peroxidation, zebularine

Project Number

2015-215Z079

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