Investigation of Powdery Mildew Susceptibility of Some Hybrid Vine Genotypes with Some Markers Related to Powdery Mildew Resistance

Yıl 2024, Cilt: 11 Sayı: 2, 506 - 513, 30.04.2024

Abdurrahim Bozkurt , Adem Yağcı

https://doi.org/10.30910/turkjans.1454926

Öz

The study was conducted in greenhouses belonging to Tokat Gaziosmanpaşa University, Agricultural Application and Research Center between 2022 and 2023. In the study, six Narince × Kishmish Vatkana (NKV) hybrids and 66 Narince x Regent (NRG) hybrids were screened for Ren1, Ren3 and Ren9 locus with markers related to powdery mildew resistance using the MAS method. The polymerase chain reaction (PCR) amplification products were visualised on an agarose gel, and the samples were examined to determine whether they exhibited a band (positive result) or not (negative result). The results of the study indicated that the band image results of the studied genes varied among the genotypes. The band image results of NRG-7, NRG-146, NRG-183, NRG-195, NRG-196, NRG-197 and NRG-200 genotypes were found to be compatible with the powdery mildew scoring results previously carried out on these genotypes. NRG-146 and NRG-195 genotypes; Ren1, Ren3, Ren9, NRG-7; Ren3 and Ren9, NRG-183 and NRG-196; Ren9, NRG-197; Ren1, Ren9 and NRG-200 genotypes gave bands (+) with respect to the Ren1 and Ren3 locus. In recent years, interest in the study of powdery mildew resistance genes or loci has increased and new resistance genes or locus have been discovered. Studies of the genes responsible for powdery mildew resistance and their mechanisms of action at the resistance locus are crucial to developing grape varieties that naturally carry resistance genes and reducing pesticide use.

Anahtar Kelimeler

Hybrid genotypes, Marker-based selection, Powdery mildew (Erysphe necator), Resistance

Proje Numarası

2022/09

Bazı Melez Asma Genotiplerinin Küllemeye Duyarlılıklarının Küllemeye Dayanıklılıkla İlişkili Bazı Markörler ile İncelenmesi

Öz

Bu çalışma 2022-2023 yılları arasında Tokat Gaziosmapaşa Üniversitesi, Tarımsal Uygulama ve Araştırma Merkezine ait seralarda yürütülmüştür. Çalışmada 6 adet Narince × Kishmish Vatkana (NKV) ve 66 adet Narince × Regent (NRG) melezi Ren1, Ren3 ve Ren9 lokusları yönünden MAS yöntemiyle küllemeye dayanıklılıkla ilişkili markörler ile taranmıştır. PCR amplifikasyon ürünleri agaroz jel üzerinde görüntülenerek, örnekler bant var (+) bant yok (-) olarak incelenmiştir. Çalışma sonucunda; genlerin bant görüntü sonuçları genotipler arasında değişkenlik göstermiştir. NRG-7, NRG-146, NRG-183, NRG-195, NRG-196, NRG-197 ve NRG-200 genotiplerinin bant görüntü sonuçları, bu genotiplerde daha önce yapılmış olan külleme skorlamaları sonuçları ile uyumlu tespit edilmiştir. NRG-146 ve NRG-195 genotipleri; Ren1, Ren3, Ren9, NRG-7; Ren3 ve Ren9, NRG-183 ve NRG-196; Ren9, NRG-197; Ren1, Ren9, ve NRG-200 genotipi Ren1 ve Ren3 lokusları bakımından bant (+) vermiştir. Son yıllarda, küllemeye karşı direnç genleri veya lokuslarının araştırılmasına olan ilgi artmış ve yeni direnç genleri veya lokusları keşfedilmiştir. Ren lokuslarında, külleme direncinden ve bunların etki mekanizmalarından hangi genlerin sorumlu olduğuna yönelik çalışmalar doğal olarak direnç genleri taşıyan üzüm çeşitlerinin geliştirilmesi, pestisit kullanımının azaltılması bakımından hayati derecede önem taşımaktadır.

Anahtar Kelimeler

Direnç genleri, Melez genotipler, külleme (Erysphe necator), Marköre dayalı seleksiyon

Etik Beyan

Çalışmamız için ‘’Etik Kurul Belgesine’’ ihtiyaç yoktur.

Destekleyen Kurum

Tokat Gaziosmanpaşa Üniversitesi Bilimsel Araştırma Fonu Başkanlığı

Proje Numarası

2022/09

Teşekkür

Bu çalışma Tokat Gaziosmanpaşa Üniversitesi Bilimsel Araştırma Fonu Başkanlığı tarafından 2022/09 nolu proje ile desteklenmiştir. Desteklerinden dolayı teşekkür ederiz.

Kaynakça

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