Sıçanlarda Akciğer Doku Yağ Asit Düzeyleri Üzerinde Kobalt Ve Silibinin Etkileri

Yıl 2024, Cilt: 11 Sayı: 3, 807 - 814, 24.07.2024

H. Turan Akkoyun

https://doi.org/10.30910/turkjans.1483278

Öz

Çalışmada kobalt ve önemli bir flavonoid olan silibin uygulanan sıçanlarda akciğer dokusunun yağ asit içeriğindeki değişimlerin belirlenmesi amaçlandı. 250±300 gr ağırlığında Wistar Albino cinsi 24 sıçan Control(0.5 mL,i.pizotonik), Kobalt(150 mg/kg/gün/oral), Silibinin(100 mg/kg/gün/oral), Kobalt+Silibinin(150 mg/kg/gün+100 mg/kg/gün/oral) olarak 4 gruba ayrıldı. Doku yağ asit analizleri GC kullanılarak gerçekleştirildi. Yağ asitleri analiz sonuçları incelendiğinde, Kontrole kıyasla genel olarak bütün gruplarda doymuş yağ asit düzeylerinde azalma gözlenirken (p>0,05, p<0,01), 16:1 n-7,18:1 n-7,18:1 n-9,18:2 n-6c,18:3 n-3, gibi doymamış yağ asitlerinde kontrole oranla kobalt uygulanan grupta artış, 15:1, 17:1, 22:5 n3, 22:6 n3 ise azalmalar tespit edildi. Silibin uygulanan grupta ise kontrole kıyasla 15:1, 17:1, 18:1 n-9, 20:4 n:6, 22:5 n3, 22:6 n:3, 20:4 n:6 yağ asitlerinde azalmalar belirlenirken bu azalmalardan bazılarının istatistiksel olarak anlamlı olduğu görüldü(p<0.01, p<0.05). Kobalt uygulanan gruba kıyasla; Kobalt+Silibinin uygulanan grupta, 24:0 yağ asit'i hariç diğer tüm doymuş yağ asitlerine (14:0, 15:0, 16:0, 17:0, 17:1, 18:0) kısmi artışlar, bütün tekli doymamış yağ asitlerinde(15:1, 16:1 n-7, 18:1 n-7, 18:1, n-9) değişen oranlarda belirgin artışlar (p<0,01, p<0,05, p<0,001) tespit edildi. Çoklu doymamış yağ asitleri kobalta kıyasla yine Kobalt +Silibinin grubunda; 18:2 n-6c yağ asidi dışındaki tüm çoklu doymamış yağ asitlerinde (18:3 n-3, 20:4 n6, 22:5 n3, 22:6 n3) belirgin artışlar tesbit edildi(p<0.05, p<0.001). Sonuç olarak; Kobalt toksisitesine karşı Silibinin uygulanan sıçan akciğer yağ asit profilini belirlemek üzere yapılan çalışmada; doymuş yağ asidi miktarlarının kontrole oranla, kobalt grubunda azalma göstermesinin bir hasar göstergesi olabileceği düşünülmektedir. Yine kobalt grubunda bazı doymamış yağ asitlerinin artış göstermesinin nedeni olduğunu düşündüğümüz yağ asidi sentezi-enzim aktivitesinin, ileri düzeyde çalışmalarla aydınlatılması gerekmektedir.

Anahtar Kelimeler

Kobalt, silibinin, sıçan, yağ asidi

Effects Of Cobalt And Silibin On Lung Tissue Fatty Acid Levels In Rats

Öz

The study aimed to determine the changes in the fatty acid content of lung tissue in rats administered cobalt and silybin, an important flavonoid. 24 Wistar Albino rats weighing 250±300 g, Control (0.5 mL, i.pisotonic), Cobalt(150mg/kg/day/oral), Silibinin(100mg/kg/day/oral), Cobalt+Silibinin(150mg/kg/day+100 mg/kg/day/oral) were divided into 4 groups. Tissue fatty acid analyzes were performed using GC. When the fatty acid analysis results were examined, a decrease in saturated fatty acid levels was observed in all groups compared to the control (p>0.05, p<0.01). There was an increase in unsaturated fatty acids such as 16:1 n-7,18:1 n-7,18:1 n-9,18:2 n-6c,18:3 n-3, in the cobalt applied group compared to the control. Decreases were detected in 15:1, 17:1, 22:5 n3, 22:6 n3. In the silybin applied group, decreases were determined in 15:1, 17:1, 18:1 n-9, 20:4 n:6, 22:5 n3, 22:6 n:3, 20:4 n:6 fatty acids compared to the control. Some of these decreases were found to be statistically significant (p<0.01, p<0.05). Compared to the cobalt applied group; In the Cobalt + Silibinin applied group, partial increases in all saturated fatty acids (14:0, 15:0, 16:0, 17:0, 18:0) except 24:0 fatty acid, all monounsaturated fatty acids were increased. Significant increases in fatty acids (15:1, 16:1 n-7, 18:1 n-7, 18:1, n-9) at varying rates (p<0.05, p<0.001) detected. Compared to cobalt, polyunsaturated fatty acids are again in the Cobalt + Silibinin group; Significant increases (p<0.05, p<0.001, p<0,01) were detected in all polyunsaturated fatty acids (18:3 n-3, 20:4 n6, 22:5 n3, 22:6 n3) except 18:2 n-6c fatty acid.
In conclusion; In the study conducted to determine the lung fatty acid profile of rats administered Silibinin against cobalt toxicity; It is thought that the decrease in the amount of saturated fatty acids in the cobalt group compared to the control may be an indicator of damage. Again, fatty acid synthesis, which we think is the reason for the increase in some unsaturated fatty acids in the cobalt group.

Anahtar Kelimeler

Cobalt, silibinin, rat, fatty acid

Kaynakça

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