Kanamisine Dayanıklı Domates Çeşitlerinde Bazı Bitki Hormonları ve Vitaminlerinin Büyümeye Etkisi

Year 2025, Volume: 12 Issue: 2, 426 - 432, 16.04.2025

İsmail Bezirganoglu , Yunus Emre Uslu

https://doi.org/10.30910/turkjans.1443141

Abstract

Domates dünya çapında yaygın olarak tüketilen bir sebzedir ve tarımsal üretim üzerinde önemli bir ekonomik etkiye sahiptir. Ancak mevcut tarımsal uygulamalardaki çeşitli zorluklar domates üretimini etkileyebilmektedir. Bu zorlukların üstesinden gelmek ve sürdürülebilir bir tarım modeli oluşturmak için domates bitkisi doku kültürüne odaklanan araştırmalar önem kazanmaktadır. Bu çalışmada neomisin fosfotransferaz (NPTII) raportör geni, A. tumefaciens LBA4404 aracılığıyla Lycopersicon esculentum cLN1558A hattına aktarıldı. Domates doku kültüründe kullanılan fenolik bileşiklerin ve büyüme düzenleyicilerin etkileri araştırıldı. Optimum sürgün rejenerasyonu için dengeli bir 1 mg ml-1 6-benziladenin (BA) ve 1 mg ml-1 gibberellik asit (GA3) konsantrasyonu kullanıldı. Optimum kök rejenerasyonu için 1 mg ml-1 indol-3-asetik asit (IAA) konsantrasyonu kullanıldı. A. tumefaciens enfeksiyonunu hızlandırmak için kültür ortamına 200 μM asetosiringon eklendi. Sonuç olarak NPTII genini içeren transgenik domates sürgünlerinde kanamisin direncinin %50'ye ulaştığı doğrulandı. Kullanılan fenolik bileşiklerin ve büyüme düzenleyicilerin etkinliği %12 artmıştır.

Keywords

Giberellik asit, 6-benzyladenine, NPTII, domates doku kültürü

Investigation Effects of Some Plant Hormones and Vitamins on Growth in Kanamycin-Resistant Tomato Varieties

Abstract

Tomato is a widely consumed vegetable around the world and has a significant economic impact on agricultural production. However, various challenges in current agricultural practices can affect tomato production. To overcome these challenges and create a sustainable agricultural model, research focusing on tomato plant tissue culture is gaining importance. In this study, the neomycin phosphotransferase (NPTII) reporter gene was transferred to the Lycopersicon esculentum cLN1558A line through A. tumefaciens LBA4404. The effects of phenolic compounds and growth regulators used in tomato tissue culture were investigated. A balanced concentration of 1 mg ml-1 6-benzyladenine (BA) and 1 mg ml-1 gibberellic acid (GA3) was used for optimal shoot regeneration. For optimal root regeneration, 1 mg ml-1 indole-3-acetic acid (IAA) concentration was used. 200 μM acetosyringone was added to the culture medium to accelerate A. tumefaciens infection. As a result, it was confirmed that kanamycin resistance reached 50% in transgenic tomato shoots containing the NPTII gene. The efficiency of the phenolic compounds and growth regulators used increased by 12%.

Keywords

Giberellic acid, 6-benzyladenine, NPTII, tomato tissue culture

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