Yumuşak Doku Onarımı için Poli(l-laktid-ko-kaprolakton) Biyobozunur Cerrahi Yamaların 3B Baskısı ve Jelatin Kaplanması

Yıl 2023, Cilt: 15 Sayı: 2, 860 - 871, 14.07.2023

Bengi Yılmaz

https://doi.org/10.29137/umagd.1285188

Öz

Yumuşak doku defektleri, kas veya bağ dokusunun zayıflaması veya bozulması nedeniyle oluşur. En sık görülen yumuşak doku hasarları arasında yer alan fıtıkların onarımında, çoğunlukla dokuyu desteklemek ve iyileşme sürecini kolaylaştırmak için, defekt bölgesine sentetik bir cerrahi yama yerleştirilir. Şimdiye kadar, mekanik performansı dokuyu desteklemeye yeterli olan, optimum doku rejenerasyonu sağlayan ve postoperatif komplikasyonları en aza indirgeyen bir cerrahi yama mevcut olmadığı için ideal malzeme ve teknik kombinasyonu arayışları devam etmektedir. Bu çalışmada, üç boyutlu (3B) basılmış makro gözenekli poli(l-laktid-ko-kaprolakton) (PLCL) ile bir mesh üretilerek, biyouyumluluğun artırılması amacıyla yüzeyi jelatin (Jel) ile kaplanmıştır. Elde edilen PLCL/Jel yamanın PLCL katmanındaki gözenek boyutunun 661,7±30,6 μm olduğu tespit edilmiştir. Yüzeyde homojen yayılmış hidrofilik karakterdeki jelatin kaplamanın su ile yüzey temas açısı 58,5±3,0° olarak kaydedilmiştir. PLCL/Jel yamaların temas açısı ise 60,5±8,8° olarak ölçülmüştür. In vitro biyobozunma deneylerinde, jelatin kaplamanın hidrolitik bozunmasının başladığı gösterilmiştir; ancak sentetik polimerin nispeten daha yavaş bozunmasına bağlı olarak, PLCL/Jel yamanın başlangıç ağırlığının %93,68±3,18'ini koruğu belirlenmiştir. Yalnız PLCL’den üretilen meshlere göre, PLCL/Jel yamanın çekme gerilimi (σM) önemli oranda artarak 6,45±1,20 MPa’a çıkmıştır ve çekme gerinimi (εM) %10,47±7,41 düzeyine inmiştir. Bu değerler, PLCL/Jel yamanın karın duvarında meydana gelen fıtıkların onarımında değerlendirilmek üzere optimum mekanik özellikler sergilediğini işaret etmektedir.

Anahtar Kelimeler

3B Baskı, Poli(l-laktid-ko-kaprolakton), Jelatin, Biyobozunur, Cerrahi Yama, Çekme Testi

Teşekkür

3B baskı ile yama üretimi, SEM, FTIR-ATR, yüzey temas açısı, in vitro biyobozunma deneyleri yazarın bağlı olduğu Sağlık Bilimleri Üniversitesi (SBÜ) Deneysel Tıp Uygulama ve Araştırma Merkezi (DETUAM) altyapısı kullanılarak gerçekleştirilmiştir. Mekanik testler ise Orta Doğu Teknik Üniversitesi (ODTÜ) Merkezi Laboratuvar’da yapılmıştır.

3D Printing and Gelatin Coating of Poly(l-lactide-co-caprolactone) Biodegradable Surgical Meshes for Soft Tissue Repair

Öz

Soft tissue defects occur due to the weakening and deterioration of muscle or connective tissues. In the repair of hernias, one of the most common types of soft tissue damage, a synthetic surgical mesh is implanted into the defect area, often to support the tissue and facilitate the healing process. Since there is no surgical mesh that has the sufficient mechanical performance to support the tissue and minimizes postoperative complications while ensuring optimal tissue regeneration, the search for the ideal combination of materials and techniques continues. In this study, a macroporous poly(l-lactide-co-caprolactone) (PLCL) mesh was 3D printed and its surface was coated with gelatin (Gel) to increase biocompatibility. It was determined that the pore size of the PLCL layer in the PLCL/Gel patch was 661.7±30.6 μm. The water contact angle of the homogeneously spread hydrophilic gelatin coating on the surface was recorded as 58.5±3.0°. The contact angle of PLCL/Gel patches was measured as 60.5±8.8°. In vitro biodegradation experiments have shown that hydrolytic degradation of the gelatin coating begins; however, due to the relatively slow degradation of the synthetic polymer, the PLCL/Gel patch retained 93.68±3.18% of its initial weight. Compared to pure PLCL meshes, the tensile strength (σM) of the PLCL/Gel was increased considerably to 6.45±1.20 MPa, and the tensile strain (εM) was reduced to 10.47±7.41. These values indicate that the PLCL/Gel patch exhibits optimum mechanical properties to be exploited in the repair of abdominal wall hernias.

Anahtar Kelimeler

3D Printing, Poly(l-lactide-co-caprolactone), Gelatin, Biodegradable, Surgical Mesh, Tensile Test

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