Aloe Vera’nın Rinitis Medikamentoza’da Nazal Mukoza Üzerine Etkisi: Deneysel Çalışma

Yıl 2022, Cilt: 48 Sayı: 1, 71 - 76, 01.04.2022

Fuat Bulut Alev Cumbul , Aylin Türksever Tetiker

https://doi.org/10.32708/uutfd.1054410

Öz

Bu çalışmamızda Aloe veranın rinitis medikamentoza (RM)‘da mukoza üzerindeki etkilerini göstermeyi amaçladık. Yaklaşık 250-300 g ağırlığında 30 adet sağlıklı 6 aylık Wistar albino sıçan kullanıldı. Bir hafta boyunca tüm hayvanlar muayene edildi, herhangi bir patoloji saptanmadı. Sıçanlar beş gruba ayrıldı. Grup 1'de 60 gün boyunca her bir burun deliğine 50 μl %0,05 oksimetazolin uygulandı. Grup 2'de, Grup 1'in kontrol grubu olarak 60 gün boyunca her gün 50 μl serum fizyolojik uygulandı. Üçüncü, dördüncü ve beşinci gruplara 60 gün boyunca günde üç kez 50 μl %0,05 oksimetazolin verildi. Grup 3, 60. günün sonunda takip eden 15 gün boyunca sadece Aloe vera solüsyonu kullandı. Grup 4'te 60. günün sonunda takip eden 15 gün boyunca sadece mometazon furoat kullanıldı. Grup 5'te tedavi kontrol grubu olarak 60. günün sonunda takip eden 15 gün serum fizyolojik kullanıldı. Nazal mukoza histopatolojik olarak değerlendirildi. Grup 3'te tunika mukozayı oluşturan epitel ve bağ dokusu Grup 2'ye güçlü bir benzerlik göstermiş ve bağ dokusunda ödem ve infiltrasyon düzeyi azalmış izlenmiştir. Grup 4'te tunika mukozanın histolojik özellikleri normale yakındı. Aloe vera yan etkisi olmayan, hem in vitro hem de in vivo antiinflamatuvar etkili bir ajandır. Sonuç olarak Aloe vera'nın RM üzerindeki iyileştirici etkisinin mometazon furoat kadar etkili olduğunu bulduk. Yaptığımız literatür araştırmaları sonucunda, bizim çalışmamız RM’de nazal mukoza üzerine Aloe veranın etkisini ortaya koyan ilk çalışmadır.

Anahtar Kelimeler

Aloe, Rinit, Mometazon Furoat, Nazal mukoza, Oksimetazolin

Destekleyen Kurum

İSTANBUL RUMELİ ÜNİVERSİTESİ REKTÖRLÜĞÜ, BİLİMSEL ARAŞTIRMA PROJELERİ KOORDİNASYON BİRİMİ

Proje Numarası

2019009

Teşekkür

Katkılarından dolayı Dilara Baban'a çok teşekkür ederiz.

Effect of Aloe Vera on the Nasal Mucosa in Rhinitis Medicamentosa: Experimental Study

Öz

In the study, we aimed to show the effects of Aloe vera on nasal mucosa in rhinitis medicamentosa (RM). 30 healthy 6-month-old Wistar albino rats weighing approximately 250-300 g were used. For one week all animals were examined, no pathology was detected. The rats were divided into five groups. In group 1, 50 μl of 0.05% oxymetazoline was applied to each nostril every day for 60 days. In Group 2, 50 μl of saline was administered every day for 60 days as the control group of Group 1. Third, fourth and fifth groups were given 50 μl of 0.05% oxymetazoline three times a day for 60 days. Group 3 used only Aloe vera solution for the following 15 days at the end of the 60th day. At the end of day 60 in Group 4, only mometasone furoate was used for the following 15 days. In group 5, saline was used for the following 15 days at the end of the 60th day as the treatment control group. Nasal mucosa was evaluated histopathologically. In Group 3, the epithelium and connective tissue forming the tunica mucosa showed a strong similarity to Group 2, and the level of edema and infiltration in the connective tissue was observed to be decreased. Histological features of tunica mucosa were close to normal in Group 4,. As a result, we found that the healing effect of Aloe vera on RM was as effective as mometasone furoate. As a result of our literature research, our study is the first to reveal the effect of Aloe vera on the nasal mucosa in RM.

Anahtar Kelimeler

Aloe, Oxymetazoline, Rhinitis, Mometasone Furoate, Nasal mucosa

Proje Numarası

2019009

Kaynakça

• 1. Graf, P. Rhinitis medicamentosa: a review of causes and treatment. Treatments in respiratory medicine 4, 21–29 (2005).
• 2. Wahid, N. W. B. & Shermetaro, C. Rhinitis Medicamentosa. in (2020).
• 3. Doshi, J. Rhinitis medicamentosa: what an otolaryngologist needs to know. European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery 266, 623–625 (2009).
• 4. Ramey, J. T., Bailen, E. & Lockey, R. F. Rhinitis medicamentosa. Journal of investigational allergology & clinical immunology 16, 148–155 (2006).
• 5. Knipping, S., Holzhausen, H. J., Goetze, G., Riederer, A. & Bloching, M. B. Rhinitis medicamentosa: electron microscopic changes of human nasal mucosa. Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery 136, 57–61 (2007).
• 6. Vogler, B. K. & Ernst, E. Aloe vera: a systematic review of its clinical effectiveness. The British journal of general practice : the journal of the Royal College of General Practitioners 49, 823–828 (1999).
• 7. Zhang, L. & Tizard, I. R. Activation of a mouse macrophage cell line by acemannan: the major carbohydrate fraction from Aloe vera gel. Immunopharmacology 35, 119–128 (1996).
• 8. Vázquez, B., Avila, G., Segura, D. & Escalante, B. Antiinflammatory activity of extracts from Aloe vera gel. Journal of ethnopharmacology 55, 69–75 (1996).
• 9. Cam, B., Sari, M., Midi, A. & Gergin, O. Xylitol treats nasal mucosa in rhinitis medicamentosa: an experimental rat model study. European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery 276, 3123–3130 (2019).
• 10. Roesler, J. et al. Application of purified polysaccharides from cell cultures of the plant Echinacea purpurea to mice mediates protection against systemic infections with Listeria monocytogenes and Candida albicans. International journal of immunopharmacology 13, 27–37 (1991).
• 11. Hoopfer, D. et al. Three-Arm Randomized Phase III Trial: Quality Aloe and Placebo Cream Versus Powder as Skin Treatment During Breast Cancer Radiation Therapy. Clinical breast cancer 15, 181–184 (2015).
• 12. Elwany, S. & Abdel-Salaam, S. Treatment of rhinitis medicamentosa with fluticasone propionate--an experimental study. European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery 258, 116–119 (2001).
• 13. Lin, C.-Y., Cheng, P.-H. & Fang, S.-Y. Mucosal changes in rhinitis medicamentosa. The Annals of otology, rhinology, and laryngology 113, 147–151 (2004).
• 14. Cumbul, A. & Bulut, F. The importance of vascular epithelial growth factor (VEGF) and inducible nitric oxide synthase (INOS) in rhinitis medicamentosa pathogenesis: An experimental rat model study. Histology and histopathology 18399 (2021). doi:10.14670/HH-18-399
• 15. Kujundzić, M. et al. Mometasone furoate and nasal vascularisation in allergic patients. Collegium antropologicum 37, 127–130 (2013).
• 16. Tas, A. et al. Use of mometasone furoate aqueous nasal spray in the treatment of rhinitis medicamentosa: an experimental study. Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery 132, 608–612 (2005).
• 17. Vaidyanathan, S., Williamson, P., Clearie, K., Khan, F. & Lipworth, B. Fluticasone reverses oxymetazoline-induced tachyphylaxis of response and rebound congestion. American journal of respiratory and critical care medicine 182, 19–24 (2010).
• 18. No Title. in Settipane RA, Kaliner MA. Nonallergic rhinitis. Am J Rhinol Allergy. 2013;27(3 suppl):S48-S51.
• 19. Mortuaire, G. et al. Rebound congestion and rhinitis medicamentosa: nasal decongestants in clinical practice. Critical review of the literature by a medical panel. European annals of otorhinolaryngology, head and neck diseases 130, 137–144 (2013).
• 20. Casale, M. et al. Topical hyaluronic acid in rhinitis medicamentosa: could our perspective be changed? Journal of biological regulators and homeostatic agents 31, 55–62 (2017).