Sıçanlarda deneysel uyku yoksunluğunun kardiyovasküler sisteme etkilerinin araştırılması

Yıl 2020, Cilt: 1 Sayı: 1, 1 - 8, 01.03.2020

Mustafa Saygın , Mehmet Özgüner , Önder Öztürk , Duygu Kumbul Doğuç , İlter İlhan , İbrahim Candan Aydın

Öz

Amaç: Bu çalışmada, deneysel olarak uyku yoksunluğu oluşturulan sıçanlarda, uyku yoksunluğunun kardiyovasküler sisteme olan etkileri araştırıldı.
Materyal ve Metod: Sıçanlar randomize olarak kontrol ve uyku yoksunluğu olarak 2 gruba ayrıldı. Grup A: kontrol grubu; deney süresince yem ve suya serbest ulaşımları sağlanarak kafeslerinde fizyolojik uykuyu uyumalarına izin verildi. Grup B: uyku yoksunluğu grubu; normal kafes içerisinde su ve yeme her zaman ulaşmalarına izin verilirken, özel bir düzenek ile 15 dakikada bir 5 dakika süresince uyaran verilip, günde 8 saat uyanık bırakıldılar. Yedi gün boyunca uyku yoksunluğu oluşturuldu. Hayvanların günlük ağırlık tartımları yapıldı ve istatistiksel olarak bir fark bulunmadı (p>0.05). Deney sonunda hayvanlar sakrifiye edilerek, tam kan sayımı, kalp dokusundan histopatolojik inceleme ve malondialdehid (MDA), süperoksid dismutaz (SOD), glutatyon peroksidaz (GPx) ve katalaz (CAT) düzeylerine bakıldı. İstatiksel değerlendirmeler, SPSS 17.0 programında, grupların homojenliğine Kolmogorov Smirnov testi ile bakıldı. Homojen dağılım gösteren gruplar, tekrarlı ölçümler, Ki-kare, bağımsız t testi ve Mann Whitney U testleri ile değerlendirilmiştir.
Bulgular: Katalaz, GPx, SOD ve MDA trombosit sayısı ve ortalama trombosit hacmi (OTH), değerleri açısından her iki grup arasında farklılık yoktur (p>0.05). Histopatolojik incelemede; her iki grup arasında myokardit (p<0.012) ve myokardiyal liflerde dejenerasyon (p<0.028) özelliği bakımından fark saptandı.
Tartışma ve Sonuç: Bu bulgular çerçevesinde, uyku yoksunluğu oluşturulan sıçanlarda oksidatif stres artmakta, bağışıklık sistemi etkilenmekte ve kardiyovasküler sistemde enflamasyona dair bulgular oluşmaktadır. Bu bağlamda, uyku yoksunluğunun uzun dönemde kardiyovasküler riskleri artırabileceğini düşünmekteyiz.

Anahtar Kelimeler

Uyku yoksunluğu, oksidatif stres, OTH, kardiyovasküler hastalıklar

Investigations of the effects of sleep deprivation on the cardiovascular system in rats

Öz

Objective: In this study, the effects of sleep deprivation on the cardiovascular system were investigated in experimentally sleep deprivation rats.
Material and method: The rats were randomly divided into 2 groups as control and sleep deprivation. Group A: control group; ensuring free
access to food and water and physiological sleep were allowed to sleep in cages throughout the experiment. Group B: sleep deprivation group;
was always permitted to reach food and water in a cage normally whether 8 hours per day were being awake through stimulus for 5 minutes
with an interval of 15 minutes by a special arrangement. Sleep deprivation was generated throughout seven days. The daily weight of the animals was weighted and there was no statistical difference between groups ( p> 0.05). At the end of the experiment the animals were sacrificed,
complete blood counts and histopathological examination of cardiac tissue, malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase ( GPx ) and catalase levels were measured. Statistical evaluations were performed by the SPSS 17.0 program and homogeneity
of the groups was analyzed with the Kolmogorov-Smirnov test. Groups which showed homogeneous distribution were evaluated by repeated
measures, chi-square, independent t-test, and Mann-Whitney U tests
Results: There were no significant differences between the two groups (p> 0.05) in terms of Catalase, GPx, SOD and MDA platelet count and
mean platelet volume (MPV) values. In histopathological examination, the difference was detected in terms of the property of myocarditis (p
<0.012) and degeneration (p <0.028) in myocardial fibers between the two groups
Discussion: Within the framework of these findings, sleep deprivation increased oxidative stress, alterations in the immune system and inflammation in the cardiovascular system. In this context, we predict that the long term sleep deprivation may increase cardiovascular risks.

Anahtar Kelimeler

Sleep deprivation, oxidative stress, MPV, cardiovascular diseases

Kaynakça

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