W locus alleles of the KIT Gene in Turkish Van Cats and Their Association with Certain Phenotypes

Yıl 2022, , 206 - 214, 30.11.2022

Mevlut Arslan , Nazlı Kocaefe-özşen Mustafa İleri

https://doi.org/10.52976/vansaglik.1141256

Öz

Objective: Turkish Van cat is special cat breed in the world. The most important characteristics of the Turkish Van cats are the white and silky fur, and different eyes color. W locus of KIT gene was found to be an important gene for the white fur. However, there is not enough information about W locus of Turkish Van cats. The aim of this study was to determine the genotypic distribution of W locus alleles in Turkish Van cats and association between alelles and certain phenotypes.
Material and Method: 48 Turkish Van cats bred in Van Yüzüncü Yıl University Van Cat Research and Application Center were selected for this study. DNA isolations were carried out from oral swaps and W locus genotyping of these cats was done by PCR. The Chi-square test was used to determine the association between the alleles and certain phenotypes.
Results: It was shown that 41.67%, 22.92%, 18.75% and 16.67% of cats carried W/W, W/w+, w+/w+and W/ws, respectively. Frequencies of W locus alleles were found to be 61.45%, 30.21%, 8.33% for W, w+, ws, respectively. An association between detected genotypes and the phenotypic characters including eye color, head spotting, and hair length, could not be established.
Conclusion: Turkish Van cats can carry white (W), white spotting (ws), and wild-type (w+) alleles in the W locus. No association between W locus alleles and eye color, head spotting, and fur length indicates other genetic variations should be addressed to understand genetical backround of the cats.

Anahtar Kelimeler

Turkish Van cat, KIT gene, W locus, FERV1, association

Van Kedilerinde KIT Geninin W locus Allelleri ve Bazı Fenotiplerle İlişkileri

Öz

Amaç: Van kedisi dünyada özel kedi ırkları arasıdadır. Van kedilerinin en önemli karakteristik özellikleri beyaz ve ipeksi tüyleri ve farklı göz renkleridir. KIT geninin W lokusu beyaz tüy için önemli bir gen olarak bulundu. Fakat, Van kedilerinin W lokusu hakkında yeterli bir bilgi bulunmamaktadır. Bu çalışmanın amacı Van kedilerinde W lokus allelerinin genotipik dağılımını ve belirli fenotipik özellikler ve alleller arasındaki ilişkiyi belirlemekti.
Materyal ve Metot: Van Yüzüncü Yıl Üniversitesi Van Kedisi Araştırma ve Uygulama Merkezinde Yetiştirilen 48 Van kedisi bu çalışma için seçildi. DNA izolasyonları ağız sürüntülerinden gerçekleştirildi ve bu kedilerin W lokus genotiplemesi PCR ile yapıldı. Ki-kare testi, alleller ve bellirli fenotipler arasında ilişkiyi belirlemek için yapıldı.
Bulgular: Kedilerin %41.67, %22.92, %18.75 ve %16.67’sinin sırasıyla W/W, W/w+, w+/w+ ve W/ws taşıdığıdı görüldü. W lokus allellerinin frenkansları W, w+, ws için sırasıyla %61.45%, 30.21 ve %8.33 olarak bulundu. Göz rengi, baş beneği ve tüy uzunluğunu içeren fenotipler ile belirlenen genotipler arasında bir ilişki saptanamadı.
Sonuç: Van kedileri W lokusda beyaz (W), beyaz beneklenme (ws) ve yabanıl tip (w+) alleleri taşıyabilir. W lokus allelleri ile göz rengi, baş beneği ve tüy uzunluğu arasında iliştkinin olmaması bu kedilerin genetik temelini anlamak için diğer genetik varyasyonlara yönlenilmesi gerektiğini işaret etmektedir.

Anahtar Kelimeler

Anahtar kelimeler: Van kedisi, KIT geni, W lokus, FERV1, İlişki

Kaynakça

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