Microbiological Risks and Portion Analyse of Stuffed Mussels Sold in Istanbul
Yıl 2020,
, 74 - 77, 03.07.2020
Burcu İrem Omurtag Korkmaz
,
Serol Korkmaz
,
Mustafa Yüce
,
Ümran Soyoğul Gürer
Öz
In this study, stuffed mussels were collected from 50 selling points in Istanbul, consist of street vendors (n=38) and retail shops (n=12), analyzed microbiologically and weighted per portion. Mean Total Aerobic Count result was 7.38±1.01 log10 cfu/g among the samples. Coliform was isolated from 44% of samples (2.85±0.57 log cfu/g), Escherichia coli from 12% of samples (3.76±0.71 log cfu/g), Staphylococcus aureus from 4% (4.15±0.30 log cfu/g), Bacillus cereus in 2% (2.78 log cfu/g) and Bacillus licheniformis in 4% (3.18±0.08 log cfu/g) of samples. Listeria ivanovii was isolated from 2% of samples (in 25 g). No Clostridium spp., Campylobacter spp., Salmonella spp. and Listeria monocytogenes were detected. Level of Staphylococcus spp. (S. aureus + S. epidermidis) contamination was significantly higher in the retail shop (P <0.05). The mean portion per stuffed mussel was 16.78±5.62 g. Except for the result of Staphylococcus spp., microbiological analyses and a gram of portions did not show a significant difference between retail shops and street vendors (P >0.05). However, an inappropriate level of S. aureus contamination in retail shops remain a food safety gap. Furthermore, the mean results of portions are assumed to be used in predictive microbiology studies.
Destekleyen Kurum
Marmara Üniversitesi Bilimsel Araştırma Projeleri Birimi (BAPKO)
Proje Numarası
SAG-C-YLP-111115-0504
Teşekkür
This study was supported by Marmara University Research Council (BAPKO, SAG-C-YLP-111115-0504). The authors are thankful for the sincere support of Esra Dalkılıç during laboratory analyses.
Kaynakça
- Ateş M, Ozkizilcik A, Tabakoglu C (2011). Microbiological analysis of stuffed mussels sold in the streets. Indian J Microbiol, 51, 350–354.
- Bingöl B, Çolak H, Hampikyan H, Muratoglu K (2008). The microbiological quality of stuffed mussels (Midye Dolma) sold in Istanbul. BFJ, 110, 1079-1087.
- Eranıl Demirli M, Tuna Ultav Z, Demirtaş-Milz N (2015). A socio-spatial analysis of urban transformation at a neighborhood scale: The case of the relocation of Kadifekale inhabitants to TOKİ Uzundere in İzmir. Cities, 48, 140–159.
- Ergönül B, Kundakçı A, Durgun S (2014). Hygienic quality of stuffed Mediterranean mussels (Mytilus gallovincialis) sold by Street venders in İzmir, Turkey. J Food Safety Food Qual, 65, 121-124.
- European Food Safety Authority (EFSA) (2012). Scientific Opinion on Public health risks represented by certain composite products containing food of animal origin. EFSA J, 10, 2662.
- Guillet C, Join-Lambert O, Le Monnier A et al. (2010). Human Listeriosis caused by Listeria ivanovii. Emerg Infect Dis, 16, 136-138.
- Güzeler N, Özbek Ç (2017). Conceptual analysis of street flavors of Turkey. Analele Universităţii din Craiova, seria Agricultură – Montanologie – Cadastru (Annals of the University of Craiova - Agriculture, Montanology, Cadastre Series) Vol. XLVII.
- ISO 6887-6:2013 (2003). Microbiology of food and animal feed -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 6: Specific rules for the preparation of samples taken at the primary production stage.
- Kişla D (2007). Effectiveness of lemon juice in the elimination of Salmonella Typhimurium in stuffed mussels. J Food Prot, 70, 2847-2850.
- Kişla D, Uzgun Y (2008). Microbiological evaluation of stuffed mussels. J Food Protect, 7, 616–620.
- Kök F, Şahiner C, Koçak P, Göksoy EÖ, Beyaz D, Büyükyörük S (2015). Determination of Microbiological Quality of Stuffed Mussels Sold in Aydin and Izmir. MJEN, 3, 70-76.
- Omurtag I, Smulders FJM, Hilbert F, Paulsen P (2012). Microbiological condition of chicken doner kebab sold in Vienna, Austria. Arch Lebensmittelhyg, 63, 142-146.
- Rhodehamel EJ, Harmon SM (2001). Clostridium perfringens. In: FDA (Ed.) Bacteriological Analytical Manual, Chapter 12. Silver Spring, Food and Drug Administration.
- Tallent SM, Rhodehamel EJ, Harmon SM, Bennett RW (2012). In: FDA (Ed.) Bacteriological Analytical Manual Chapter 14, Bacillus cereus. Food and Drug Administration.
- Terzi G, Gücükoğlu A, Çadirci Ö, Uyanik T, Alişarli M (2015). Serotyping and antibiotic susceptibility of Listeria monocytogenes isolated from ready-to-eat foods in Samsun, Turkey. Turk J Vet Anim Sci, 39, 211-217.
- Thepaut J, Soriano H (2012). Comparison of BACARA® agar, a new chromogenic medium and MYP agar for the enumeration of B.cereus in food sample. Aes Chemunex-BioMérieux.
- Turkish Food Codex (TFC) (2011). Turkish Food Codex, Regulation of microbiological criteria. Nr. 28157.
- Ulusoy S, Ozden O (2011). Preservation of stuffed mussels at 4 degrees C in modified atmosphere packaging. J Aquat Food Prod Technol, 20, 319-330.
- Urazel B, Çelikel A, Karbeyaz K, Akkaya H (2014). The evaluation of forensic cases reported due to food poisoning. Dicle Med J, 41, 113-117.
- Yılmaz H, Bostan K, Turan K et al. (2010). RealTime PCR detection of Norovirus in mussels collected from the bosphorus in Istanbul, Turkey. Food Environ Virol, 2, 64-68.
İstanbul’da Satılan Midye Dolmalardaki Mikrobiyolojik Riskler ve Porsiyon Analizi
Yıl 2020,
, 74 - 77, 03.07.2020
Burcu İrem Omurtag Korkmaz
,
Serol Korkmaz
,
Mustafa Yüce
,
Ümran Soyoğul Gürer
Öz
Bu çalışmada İstanbul’da sokakta (n=38) ve kapalı restoranda (n=12) satışa sunulan toplam 50 farklı noktadan midye dolma örneği toplanarak mikrobiyolojik inceleme ve porsiyon analizi yapılmıştır. Örneklerin Toplam Aerobik Bakteri sayısı ortalama 7.38±1.01 log10 kob/g olarak bulunmuştur. Coliform grubu %44 (2.85±0.57 log kob/g), Escherichia coli %12 (3.76±0.71 log kob/g), Staphylococcus aureus %4 (4.15±0.30 log kob/g), Bacillus cereus %2 (2.78 log cfu/g) ve Bacillus licheniformis %4 (3.18±0.08 log cfu/g) oranında izole edilmiştir. Listeria ivanovii %2 oranında tespit edilmiştir. Clostridium spp., Campylobacter spp., Salmonella spp. ve Listeria monocytogenes tespit edilmemiştir. Staphylococcus spp. (S. aureus + S. epidermidis) kontaminasyon düzeyi restoranlarda anlamlı olarak daha yüksek bulunmuştur (P <0.05). Midye dolmaların adet porsiyonu ortalama 16.78±5.62 g olarak tartılmıştır. Staphylococcus spp. sonuçları dışında mikrobiyolojik analizler ve porsiyon gramajları restoranlar ile sokak satış noktaları arasında anlamlı bir fark göstermemiştir (P >0.05). Ancak S.aureus’un kapalı restoranlarda tüketim için uygun düzeyin üzerinde tespit edilmesi bir gıda güvenliği açığının bulunduğuna işaret etmektedir. Porsiyon ortalamalarının ileride prediktif mikrobiyoloji çalışmalarında kullanılabileceği öngörülmektedir.
Proje Numarası
SAG-C-YLP-111115-0504
Kaynakça
- Ateş M, Ozkizilcik A, Tabakoglu C (2011). Microbiological analysis of stuffed mussels sold in the streets. Indian J Microbiol, 51, 350–354.
- Bingöl B, Çolak H, Hampikyan H, Muratoglu K (2008). The microbiological quality of stuffed mussels (Midye Dolma) sold in Istanbul. BFJ, 110, 1079-1087.
- Eranıl Demirli M, Tuna Ultav Z, Demirtaş-Milz N (2015). A socio-spatial analysis of urban transformation at a neighborhood scale: The case of the relocation of Kadifekale inhabitants to TOKİ Uzundere in İzmir. Cities, 48, 140–159.
- Ergönül B, Kundakçı A, Durgun S (2014). Hygienic quality of stuffed Mediterranean mussels (Mytilus gallovincialis) sold by Street venders in İzmir, Turkey. J Food Safety Food Qual, 65, 121-124.
- European Food Safety Authority (EFSA) (2012). Scientific Opinion on Public health risks represented by certain composite products containing food of animal origin. EFSA J, 10, 2662.
- Guillet C, Join-Lambert O, Le Monnier A et al. (2010). Human Listeriosis caused by Listeria ivanovii. Emerg Infect Dis, 16, 136-138.
- Güzeler N, Özbek Ç (2017). Conceptual analysis of street flavors of Turkey. Analele Universităţii din Craiova, seria Agricultură – Montanologie – Cadastru (Annals of the University of Craiova - Agriculture, Montanology, Cadastre Series) Vol. XLVII.
- ISO 6887-6:2013 (2003). Microbiology of food and animal feed -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 6: Specific rules for the preparation of samples taken at the primary production stage.
- Kişla D (2007). Effectiveness of lemon juice in the elimination of Salmonella Typhimurium in stuffed mussels. J Food Prot, 70, 2847-2850.
- Kişla D, Uzgun Y (2008). Microbiological evaluation of stuffed mussels. J Food Protect, 7, 616–620.
- Kök F, Şahiner C, Koçak P, Göksoy EÖ, Beyaz D, Büyükyörük S (2015). Determination of Microbiological Quality of Stuffed Mussels Sold in Aydin and Izmir. MJEN, 3, 70-76.
- Omurtag I, Smulders FJM, Hilbert F, Paulsen P (2012). Microbiological condition of chicken doner kebab sold in Vienna, Austria. Arch Lebensmittelhyg, 63, 142-146.
- Rhodehamel EJ, Harmon SM (2001). Clostridium perfringens. In: FDA (Ed.) Bacteriological Analytical Manual, Chapter 12. Silver Spring, Food and Drug Administration.
- Tallent SM, Rhodehamel EJ, Harmon SM, Bennett RW (2012). In: FDA (Ed.) Bacteriological Analytical Manual Chapter 14, Bacillus cereus. Food and Drug Administration.
- Terzi G, Gücükoğlu A, Çadirci Ö, Uyanik T, Alişarli M (2015). Serotyping and antibiotic susceptibility of Listeria monocytogenes isolated from ready-to-eat foods in Samsun, Turkey. Turk J Vet Anim Sci, 39, 211-217.
- Thepaut J, Soriano H (2012). Comparison of BACARA® agar, a new chromogenic medium and MYP agar for the enumeration of B.cereus in food sample. Aes Chemunex-BioMérieux.
- Turkish Food Codex (TFC) (2011). Turkish Food Codex, Regulation of microbiological criteria. Nr. 28157.
- Ulusoy S, Ozden O (2011). Preservation of stuffed mussels at 4 degrees C in modified atmosphere packaging. J Aquat Food Prod Technol, 20, 319-330.
- Urazel B, Çelikel A, Karbeyaz K, Akkaya H (2014). The evaluation of forensic cases reported due to food poisoning. Dicle Med J, 41, 113-117.
- Yılmaz H, Bostan K, Turan K et al. (2010). RealTime PCR detection of Norovirus in mussels collected from the bosphorus in Istanbul, Turkey. Food Environ Virol, 2, 64-68.