Endotoksemi Şekillendirilmiş Ratlarda Marbofloksasin, Diklofenak Sodyum ve Metilprednizolonun Serum Biyokimyasal Değerler Üzerine Etkisi

Yıl 2021, , 98 - 103, 26.11.2021

Ahmet Cihat Öner , Ali Şahin

https://doi.org/10.36483/vanvetj.948037

Cited By: 1

Öz

Escherichia coli'den türetilen lipopolisakkarit (LPS), sepsis ve septik şok için bir model olarak yaygın olarak kullanılmıştır. Çalışmamızda LPS ile deneysel endotoksemi oluşturulan ratlarda, marbofloksasin, diklofenak sodyum, metilprednizolon kullanılarak, bu ilaçların organ yetmezliğinin indirekt belirteçleri olan alkalen fosfotaz (ALP), alanin aminotransferaz (ALT), aspartat aminotransferaz (AST), gama glutamil transferaz (GGT), kan üre azot (BUN), kreatinin değerleri üzerine olan etkilerinin değerlendirilmesi amaçlanmıştır. Çalışma için gerekli 186 adet rat, 5 gruba ayrıldı. Kontrol grubundan 0. saatte kan örnekleri alındı. Ratlarda endotoksemi oluşturmak amacı ile intraperitoneal (IP) yolla LPS (4mg/rat) uygulandı. Gelişen endotoksemiyi tedavi etmek için marbofloksasin IP yolla 100 mg/kg, diklofenak sodyum IP yolla 10 mg/kg, metilprednizolon IP yolla 10 mg/kg dozunda uygulandı. Daha sonra 1, 2, 4, 8, 12 ve 24. saatlerde tiyopental anestezisi altında kan örnekleri alınarak biyokimyasal değerler ölçüldü. Çalışmada serum ALP, ALT, AST, GGT, BUN ve kreatinin düzeylerinin LPS uygulaması ile arttığı (P<0.05) ve sepsiste beklenilen etkinin şekillendiği tespit edildi. Sepsis tedavisinde, metilprednizolon dışında diğer ilaçların tek başlarına kullanılamayacağı ancak kombine uygulamanın tercih edilebileceği sonucuna ulaşıldı.

Anahtar Kelimeler

Diklofenak sodyum, endotoksemi, metilprednizolon.

Destekleyen Kurum

Bu araştırma Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı

Proje Numarası

2010-SBE-D174

Teşekkür

Bu araştırma Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı tarafından 2010-SBE-D174 no’lu proje ile desteklenmiştir

Effects of Marbofloxacin, Diclofenac Sodium And Methylprednisolone on Serum Biochemical Values in Endotoxemia-Shaped Rats

Öz

Escherichia coli-derived lipopolysaccharide (LPS) has been used extensively as a model for septic shock and sepsis. In our study, it was aimed to induce experimental endotoxemia in rats by using LPS and the effects of marbofloxacin, diclofenac sodium and methylprednisolone on alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma glutamyl transferase (GGT), blood urea nitrogen (BUN), creatinine, which are indirect indicators of organ insufficiency, were assessed. 186 rats were divided into 5 groups. Blood samples were obtained from control group at 0 h. To shape endotoxemia, LPS was administered at a dose of 4 mg/rat via intraperitonally (IP). Marbofloxacin (100 mg/kg, IP), diclofenac sodium (10 mg/kg, IP) and metylprednisolone (10 mg/kg, IP) were administered at dosage for the treatment of developing endotoxemia. Blood samples were collected under thiopental anesthesia at 1, 2, 4, 8, 12 and 24 h. They were analyzed biochemical levels. In the study, it was determined that serum ALP, ALT, AST, GGT,BUN and creatinine levels increased with LPS application (P<0.05) and the expected effect in sepsis was formed. As a result, LPS administration caused sepsis and it had several effects observed in the kidney and liver. It was concluded that in the treatment of sepsis, drugs other than methylprednisolone cannot be used alone, but combined application can be preferred.

Anahtar Kelimeler

Diclofenac sodium, endotoxemia, methylprednisolone.

Proje Numarası

2010-SBE-D174

Kaynakça

• Berger MM, Chiolero RL (2007). Antioxidant supplementation in sepsis and systemic inflammatory response syndrome. Crit Care Med, 35, 584-590.
• Bonelli F, Meucci V, Divers TJ, Boccardo A, Pravettoni D ve ark. (2018). Plasma procalcitonin concentration in healthy calves and those with septic systemic inflammatory response syndrome. Vet J, 234, 61-65.
• Boscolo P, Youinou P, Theoharides TC, Cerulli G, Conti P (2008). Environmental and occupational stress and autoimmunity. Autoimmun Rev, 7, 340-343.
• Camcıoğlu Y, Aytaç E (2007). Sepsisin İmmunopatogenezi. Türk Yoğun Bakım Dergisi, 5, 81-85.
• Constable PD, Kenneth W, Hinchcliff KW, Done SH, Grünberg W (2017). Veterinary Medicine. A Textbook of the Diseases of Cattle, Sheep, Pigs, Goats and Horses. 11th ed., Saunders, Missouri.
• Dawulieti J, Sun M, Zhao Y, Shao D, Yan H, et all (2020). Treatment of severe sepsis with nanoparticulate cell-free DNA scavengers, Sci Adv, 6, 7148.
• Elmas M, Yazar E, Uney K, Er Karabacak A (2006). Influence of Escherichia coli endotoxin induced endotoxaemia on the pharmacokinetics on enrofloxacin after intravenous administration in rabbits. J Vet Med A, 53, 410-414.
• Elmas M, Yazar E, Uney K, Er Karabacak A, Traş B (2008). Pharmacokinetics of enrofloxacin and flunixin meglumine and interactions between both drugs after intravenous co-administration in healthy and endotoxaemic rabbits. Vet J, 177, 418-424.
• Hart KA, MacKay RJ (2015). Endotoxemia and sepsis. In: Smith BP (Editor). Large Animal Internal Medicine (pp.682-695). 5th Edition, St.Louis, Missouri: Elsevier.
• James PE, Madhani M, Roebuck W, Jackson SK, Swartz HM (2002). Endotoxin-induced liver hypoxia: Defective oxygen delivery versus oxygen consumption. Nitric Oxide, 6, 18-28.
• Kerr MG (2002). İndividual enzyme interpretation (pp.101-147). Veterinary Laboratuary Medicine, Blackwell Science, 2th edit.
• Lelubre C, Vincent JL (2018). Mechanisms and treatment of organ failure in sepsis. Nat Rev Nephrol, 14 (7), 417-27.
• Lukashenko PV, Lukashenko TM, Savanovich II, Sandakov DB, Gerein V (2004). Granulocyte macrophage colony-stimulating factor regulates activity of the nervous system. Neuroimmunomodulation, 11, 36-40.
• Meduri GU (1999). An historical review of glucocorticoid treatment in sepsis. Disease pathophysiology and the design of treatment investigation. Sepsis, 3, 21-38.
• Minneci PC, Deans KJ, Banks SM, Eichacker PQ, Natanson C (2004). Meta-Analysis: The effect of steroids on survival and shock during sepsis depends on the dose. Ann Intern Med, 141 (1), 47-58.
• Pardon B, Deprez P (2018). Rational antimicrobial therapy for sepsis in cattle in face of the new legislation on critically important antimicrobials. Vlaams Diergen Tijds, 87, 37-46.
• Pastor CM, Billiar TR, Losser MR, Payen DM (1995). Liver injury during sepsis. J Crit Care Med, 10, 183-197.
• Prauchner CA (2017). Oxidative stress in sepsis: Pathophysiologicalimplications justifying antioxidant co-therapy, Burns, 43, 471-485.
• Swarnalatha Y, Sivakkumar SU, Siddharthan S (2020). Protective role of heptamethoxyflavone on LPS-induced hepatotoxicity. Toxin Reviews.
• Traş B, Yazar E, Elmas M (2007). Veteriner Hekimliğinde İlaç Kullanımına Pratik ve Akılcı Yaklaşım. Olgun Çelik Ofset Matbaası, Konya.
• Turgut K (2000). Veteriner Klinik Laboratuvar Teşhis. Bahçıvanlar A.Ş, Konya.
• Wedn AM, El-Gowilly SM, El-Mas MM (2020). Time and sex dependency of hemodynamic, renal, and survivability effects of endotoxemia in rats. Saudi Pharmaceutical Journal, 28, 127-135.
• Wang CC, Lee YM, Wei HP, Chu CC, Yen MH (2004). Dextromethorphan prevents circulatory failure in rats with endotoxemia. J Biomed Sci, 11, 739-747.
• Yarema TC, Yost S (2011). Low-Dose Corticosteroids to Treat Septic Shock: A Critical Literature Review. Crit Care Nurse, 31, 16-26.
• Yazar E, Çöl R, Konyalıoğlu S ve ark. (2004). Effects of vitamin E and prednisolone on biochemical and haematological parameters in endotoxemic New Zealand white rabbits. B Vet I Pulawy, 48, 105-108.
• Yazar E, Elmas M, Traş B, Üney K, Er A (2009). Septik şok tedavisinde kullanılan ilaçların şokun patofizyolojisine etkileri. Tübitak Projesi, Proje No: 107O042.
• Yazar E, Er A, Uney K, Bulbul A, Avcı GB ve ark. (2010). Effects of drugs used in endotoxic shock on oxidative stress and organ damage markers. Free Rad Res, 44, 397-402.