Effects of N Acetylcysteine on Expression and Localization of Some Lectins in The Liver of Experimental Hepatic Intoxication

Yıl 2016, Cilt: 27 Sayı: 1, 11 - 16, 20.04.2016

Dua Danjollı Hasan Akşit

Öz

The aim of this study was to investigate the effects of intraperitoneal applications NAC that is a GSH precursor,determine the effects of some lectins expression and localization of the liver. Liver toxicity of CCl₄in rats in order to create, intraperitoneal (i.p.) 1 ml/kg 1/1 ratio of olive oil in the form of solution injected 3 times with an interval of one day. 24 hours after CCl₄injection, liver tissue taken under ether anesthesia. In order to determine the protective effect of N Acetyl Cysteine, N Acetyl Cysteine application (i.p. 50 mg/kg/day) 3 days prior to CCl₄injection started and continued during the experimental period. Animals liver tissue were taken 24. hours after the last injection under the ether anesthesia. Galactose (EEL), Lactose (R II) and Mannose (GNL) specific oligosaccharides were determined using biotinylated labeled lectins in liver tissue sections. Evaluation of the reaction was performed by light microscopy. Histochemically, the reactions have been observed in centrilobular vein sinusoidal capillaries of liver tissue. No staining was detected in hepatocytes. Compared with controls, CCl₄group were more intense staining. NAC reduced the staining intensity. As a result, cells increase their oligosaccharide units to protect them self from the liver damage of CCl₄. It was observed that NAC had protective and therapeutic effects against damage induced by CCl₄in rats hepatic cells.

Anahtar Kelimeler

CCl4, EEL, GNL, NAC, Rat, RCA I

Deneysel Karaciğer İntoksikasyonunda N Asetil Sisteinin Karaciğerdeki Bazı Lektinlerin Ekspresyonu ve Lokalizasyonu Üzerine Etkileri

Öz

Yapılan çalışmada karaciğer dokusunda, GSH öncüsü NAS intraperitonal uygulamalarının, karaciğerdeki bazı lektinlerin ekspresyonu ve lokalizasyonu üzerine etkilerinin belirlenmesi amaçlanmıştır. Karaciğer toksisitesi oluşturmak amacıyla, sıçanlara CCl_4,periton içi (i.p.) 1 ml/kg 1/1 oranında, zeytinyağındaki çözeltisi şeklinde, birer gün ara ile 3 defa enjekte edildi. CCl₄enjeksiyonundan 24 saat sonra eter anestezisi altında, karaciğer dokusu alındı. N Asetil Sisteinin koruyucu etkisinin belirlenmesi amacıyla, NAS uygulaması (periton içi 50 mg/kg/gün) CCl₄enjeksiyonundan 3 gün önce başladı ve deney süresince devam etti. Hayvanların sonenjeksiyondan 24 saat sonra eter anestezisi altında karaciğer dokuları alındı. Karaciğer dokusundan alınan kesitlerde Galaktoza (EEL), Laktoza (RCA I) ve Mannoza (GNL)spesifik oligosakkarit üniteleribiotinlenmişişaretli lektinlerin kullanılması ile belirlendi.Reaksiyonların değerlendirilmesi ışık mikroskobunda gerçekleştirildi. Histokimyasal olarak reaksiyonlar; sentralobüler damar ve karaciğer dokusunun, sinüsoidal kapillerlerinde gözlemlendi. Hepatositlerde boyanma tespit edilmedi. Kontrollere göre CCl₄grubunda, daha yoğun bir boyama tespit edildi. NAS ise boyama yoğunluğunu azalttı. Sonuç olarak, hücrelerin CCl₄’e bağlı karaciğer hasarından kendilerini korumak için oligosakkarit ünitelerini artırdığı görüldü. NAS’ın, sıçanların hepatik hücrelerinde CCl₄ile oluşturulan hasara karşı koruyucu ve tedavi edici etkileri olduğu gözlemlendi.

Anahtar Kelimeler

CCl4, EEL, GNL, NAS, Rat, RCA I

Kaynakça

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