Effect of Platelet Rich Plasma on Intraabdominal Adhesions in Rats

Yıl 2024, Cilt: 35 Sayı: 1, 1 - 6, 29.03.2024

Mustafa Köm , İlyas Akay Eren Polat , İlknur Çalık

https://doi.org/10.36483/vanvetj.1385092

Öz

Despite the development of surgical technologies, intraabdominal adhesions are one of the most important problems for infertility, abdominopelvic pain, and intestinal obstruction. This study was aimed the efficiency of PRP (Platelet Rich Plasma) on preventing postoperative intraabdominal adhesions. In this study, 25 male Sprak Dawley breed rats of 4 months old were used. They were divided into 2 groups equally. Five rats were used as donor and obtained PRP. Following general anesthesia and under routine procedures, all groups were traumatized 1 cm2 area on the ileocecal region while group 1 was served as control with no chemical, the group 2 was locally applied PRP. Relaparatomy was made on the 14 days after surgery The results were evaluated macroscopically and histopathologically. Adhesion and fibrosis scores of PRP group were observed to have significantly decreased compared to the control (p<0.05). There was no significant difference in inflamation between the PRP and control groups (p>0.05). In conclusion, it was determined that PRP was nore effective on the preventive of intraabdominal adhesions.

Anahtar Kelimeler

Intraabdominal adhesion, PRP, Rat

Ratlarda İntrabdominal Adezyonların Üzerine Trombositten Zengin Plazma’nın Etkisi

Öz

Cerrahi alanındaki teknolojik gelişmelere rağmen intraabdominal adezyonlar; kısırlık, abdominaopelvik ağrı ve bağırsak tıkanıklıklarının en önemli nedenlerinden biridir. Bu çalışmada; trombositten zengin plazma (PRP)’nin intraabdominal adezyonların önlenmesindeki etkinliğinin ortaya konulması amaçlandı. Çalışmada 4 aylık 25 adet erkek Spraque Dawley ırkı rat kullanıldı. Ratlar her grupta 10 rat olmak üzere iki eşit gruba ayrıldı. Beş rat ise donör olarak kullanıldı. Bu ratlardan elde edilen kanlardan RRP hazırlandı. Genel anestezi altında operasyonlar klasik cerrahi kurallar altında iliosekal bölge açığa çıkarıldı ve 1 cm2’lik bir bölge travmatize edildi. Birinci grup kontrol olarak değerlendirildi ve herhangi bir kimyasal uygulanmadı. İkinci gruptaki ratlara PRP 0.4 ml dozunda lokal olarak uygulandı. Postoperatif 14. günde relaparatomi yapıldı. Sonuçlar makroskopik ve histopatolojik olarak değerlendirildi. PRP grubunun adezyon derecesi ve fibrozis dereceleri kontrol grubu ile karşılaştırıldığında istatistiksel olarak anlamlı olduğu saptandı (p<0.05). PRP ve kontrol grupları arasında inflamasyon derecesi bakımından anlamlı bir farkın olmadığı gözlendi (p>0.05). Sonuç olarak, PRP’nin intraabdominal adezyonları önlemede daha etkili olduğu saptandı.

Anahtar Kelimeler

İntraabdominal Adezyon, PRP, Rat

Kaynakça

• Abdullah BJ, Atasoy N, Omer AK (2019). Evaluate the effects of platelet rich plasma (PRP) and zinc oxide ointment on skin wound healing. Ann Med Surg, 37, 30–37.
• Abegao KGB, Bracale BN, Delfim IG et al. (2015). Effects of heterologous platelet-rich plasma gel on standardized dermal wound healing in rabbits. Acta Cir Bras, 30 (3), 209-215.
• Akbarzadeh S, McKenzie MB, Rahman M, Cleland H (2021). Allogeneic platelet-rich plasma: is it safe and effective for wound repair? Eur Surg Res, 62, 1–9.
• Alkan F, Koç Y, Çelik İ, Erol M, Aydın MF (2007). Tavşanlarda peritoneal adezyonların önlenmesinde metilprednisolon (MP) ve dimetil sülfoksit (DMSO)’in etkilerinin araştırılması. Vet Bil Derg, 21 (2), 73-79.
• Altıntaş Ural D, Altıntaş Aykan D, Seyithanoğlu M et al. (2022). Efficacy of pure olive oil and PRF in the prevention of postoperative peritoneal adhesions. Cukurova Med J, 47 (2), 570-579.
• Alves R, Grimalt RA (2018). review of platelet-rich plasma: history, biology, mechanism of action, and classification. Skin Appendage Disord, 4 (1), 18-24.
• Arnoczky SP, Shebani-Rad S (2013). The basic science of platelet-rich plasma (PRP): what clinicians need to know. Sports Med Arthrosc Rev, 21, 180-185.
• Arung W, Meyrisse M, Detry O (2011). Pathophysiology and prevention of postoperative peritoneal adhesions. World J Gastroenterol, 17 (41), 4545-4553.
• Belebecha V, Casagrande R, Mariana R et al. (2020). Effect of the platelet-rich plasma covering of polypropylene mesh on oxidative stress, inflammation, and adhesions. Int Urogynecol J, 31, 139-147.
• Beyene RT, Kavalukas SL, Barbul A (2015). Intra-abdominal adhesions: anatomy, physiology, pathophysiology, and treatment. Curr Probl Surg, 52 (7), 271-319.
• Boland GM, Weigel RJ (2006). Formation and prevention of postoperative abdominal adhesions. J Surg Res, 132 (1), 3-12.
• Collins T, Alexander D, Barkatali B (2021). Platelet-rich plasma: a narrative review. EFORT Open Rev, 6 (4), 225-235.
• Daradka M, Alardah MM, Ismail ZB (2019). Effects of autologous platelet-rich plasma coated sutures on intestinal anastomotic healing in rabbits. Heliyon, 5 (11), e02713.
• De Pascale MR, Linda Sommese L, Casamassimi A, Napoli C (2015). Platelet derivatives in regenerative medicine: an update. Transfus Med Rev, 29 (1), 52-61.
• Fresno L, Fondevila D, Bambo O (2010). Effects of platelet-rich plasma on intestinal wound healing in pigs. Vet J, 185 (3), 322-327.
• Ghobrial S, Ott J, Parry JP (2023). An overview of postoperative intraabdominal adhesions and their role on female infertility: a narrative review. J Clin Med, 12 (6), 1-14.
• Günay C, Sağlıyan A, Yaman İ (2005). Ratlarda deneysel olarak oluşturulan intraabdominal adezyonların önlenmesinde aprotinin ile metilen mavisinin etkinliğinin karşılaştırılması. FÜ Sağ Bil Derg, 2005, 19 (1), 51-55.
• Hay WP, Mueller PO, Harmon B, Amoroso L (2001). One percent sodium carboxymethylcellulose prevents experimentally induced abdominal adhesions in horses. Vet Surg, 30 (3), 223-227.
• Hooker GD, Taylor BM, Driman DK (1999). Prevention of adhesion formation with use of sodium hyaluronate-based bioresorbable membrane in a rat model of ventral hernia repair with polypropylene mesh. a randomized, controlled study. Surgery, 125, 211-216.
• Karakaş DÖ, Dandin Ö, Müftüoğlu T et al. (2020). Effect of platelet-rich plasma on postoperative peritoneal inflammation and adhesions. Arch Med Sci, 17 (5), 1408-1413.
• Kaya F, Kismet K, Ozer H et al. (2016). Can platelet-rich plasma be used safely in intra-abdominal operations? Bratisl Med J, 117 (9), 525-529.
• Kamel RM (2010). Prevention of postoperative peritoneal adhesions. Eur J Obstet Gyn R B, 150, 111-118.
• Köm M (2013). Effect of Hyaluronic acid/carboxymethylcellulose and Flunixin Meglumine Combination on the Prevention of Postoperative Intraabdominal Adhesions (an experimental study in rabbits). Kafkas Univ Vet Fak Derg, 19 (4), 613-618.
• Köm M (2015). Tavşanlarda postoperatif intraabdominal adezyonların önlenmesinde hyalüronik asit/karboksimetilselüloz bariyerlerin etkinliği. FÜ Sağ Bil Derg, 29 (2), 75-79.
• Le ADK, Enweze L, DeBaun MR, Drago JL (2019). Platelet-rich plasma. Clin Sports Med, 38 (1), 17-44.
• Makarchian HR, Kasraianfard A, Ghaderzadeh P, Javadi SMR, Ghorbanpoor M (2017). The effectiveness of heparin, platelet-rich plasma (PRP), and silver nanoparticles on prevention of postoperative peritoneal adhesion formation in rats. Acta Cir Bras, 32 (1), 22-27.
• Marques LF, Stessuk T, Camargo IC et al. (2015). Platelet-rich plasma (PRP): methodological aspects and clinical applications. Platelets, 26 (2), 101-113.
• Moll HD, Schumacher J, Spano WJC (1991). Evaluation of sodium carboxymethylcellulose for prevention of experimentally induced abdominal adhesions in ponies. Am J Vet Res, 52 (1), 88-91.
• Moll HD, Wolfe DF, Schumacher J, Write JC (1992). Evaluation of sodium carboxymethylcellulose for prevention of adhesions after uterine trauma in ewes. Am J Vet Res, 53 (8), 1454-1456.
• Mueller POE (2002). Advances in prevention and treatment of intra-abdominal adhesions in horses. Clin Tech Equine Pract, 1 (3), 163-173.
• Nair SK, Bhat IK, Aurora AL (1974). Role of proteolytic enzyme in the prevention of postoperative intraperitoneal adhesions. Arch Surg, 108, 849-853.
• Nikolovska B, Miladinova D, Pejkova S et al. (2021). Platelet-rich plasma-review оf literature. Pril, 42 (1), 127-139.
• Oneto P, Etulain J (2021). PRP in wound healing applications. Platelets, 32 (2), 189-199.
• Öztan MO, Arslan FD, Oztan S, Diniz G, Köylüoğlu G (2019). Effects of topical application of platelet-rich plasma on esophageal stricture and oxidative stress after caustic burn in rats: Is autologous treatment possible? J Pediatr Surg, 54 (7), 1397-1404.
• Schnüriger B, Barmparas G, Branco B et al (2011). Prevention of postoperative peritoneal adhesions: a rewiew of the literature. Am J Surg, 201, 111-121.
• Smith OJ, Jell G, Mosahebi A (2019). The use of fat grafting and platelet-rich plasma for wound healing: A review of the current evidence. Int Wound J, 16 (1), 275-285.
• Tang J, Xiang Z, Bernards MT, Chen S (2020). Peritoneal adhesions: occurrence, prevention and experimental models. Acta Biomater, 116, 84-104.
• Trew G (2006). Postoperative adhesions and their prevention. Reviews in Gynaecological and Perinatal Practice, 6, 47-56.
• Turan E, Ayhan B, Kargın S, Doğru O, Uğraş NS (2020). Evaluation of the efficacy of platelet-rich plasma in preventing postoperative intraabdominal adhesions. Turk J Surg, 36 (1), 53-58.
• Ward BC, Panintch A (2011). Abdominal adhesions: current and novel therapies. J Surg Res, 165, 91-111.
• Yıldız H, Durmuş AS, Şimşek H, Yaman İ (2011). The comparison of methylene blue and vitamin E in prevention of abdominal postoperative adhesion formation in rat uterine horn models. biochemical and histopathologic evaluation. Acta Cir Bras, 26 (1), 51-57.
• Yilmaz Y, Çelik İH, Pampal A (2012). Effects of different pulmonary surfactants in the prevention of postoperative intraabdominal adhesion formation. J Pediatr Surgy, 47, 1560–1565.
• Zhang W, Guo Y, Kuss M et al. (2019). Platelet-rich plasma for the treatment of tissue infection: preparation and clinical evaluation. Tissue Eng Part B Rev, 25 (3), 225-236.