Ketamine-Ksilazin Anestezisi Uygulanmış Bozova Tazılarında Oksidatif Stres Parametreleri

Yıl 2016, Cilt: 1 Sayı: 1, 26 - 30, 11.12.2016

Güzin Camkerten , İlker Camkerten , Nihat Şındak Gaye Bulut

Öz

Bu
çalışma ksilazin-ketamin anestezisinin Bozova tazılarında bazı iz element (Cu,
Zn, Mn) ve oksidatif stres parametreleri (SOD, CAT, GPx, MDA) üzerine
etkilerini değerlendirmek amacıyla yapıldı. Farklı yaş, vücut ağırlığı ve
cinsiyetteki toplam sekiz tazı kullanıldı. Farklı şikâyetlerle gelen bu hayvanlara
Ketamin (10mg/kg) ve ksilazin (1 mg / k g) kas içi enjekte edildi. Anestezi
öncesi ve anestezi sırasında antikoagulanlı ve antikoagulansız tüplere kan
örnekleri alındı. Kan örnekleri 3000 rpm’de 10 dk santrifüje edilerek aynı gün
değerlendirildi. Eritrosit süperoksit dismutaz (SOD), Glutatyon Peroksidaz
(GPx), malondialdehit (MDA) ve plazma Katalaz (CAT) değerleri standart
metotlarla, serum bakır, çinko, manganez düzeyleri ICP-OES ile belirlendi. Bu
çalışmada; SOD aktivitesinin anestezi sonrasında düşük (p<0,05) olduğu
belirlenirken GPx, MDA,  CAT, Cu, Zn ve
Mn seviyelerinde istatistiksel olarak önemli bir fark belirlenmedi (p>0.05).
Sonuç olarak, ketamin ve ksilazin anestezisinin Bozova tazılarında serum Cu,
Zn, Mn seviyeleri ve oksidatif stres yönünden güvenli ve kullanılabilir olduğu
söylenebilir.

Anahtar Kelimeler

Bozova tazısı, oxidatif stres, SOD, CAT, GPx, MDA, Cu, Zn

Oxidative Stress Parameters in Xylasine-Ketamine Adminestered Bozova Greyhounds

Öz

The
present study was conducted to evaluate the effects of xylaine-ketamine
anesthezia on some micro elements and oxidative stress parameters in Bozova
greyhounds. In the study eight male greyhounds, which were in different age and
body weight were used as the material. The animals, which were, came in clinic
with different complaints injected with the ketamine (10 mg/kg) and xylazine (1
mg/kg) intramuscularly. The findings were recorded before and during
anesthesia. Blood samples were collected from the vena cephalica. Serum copper,
zinc, manganese levels were determined by an ICP-OES. Superoxide dismutase (SOD), glutathione
peroxidase (GPx), malondialdehyde (MDA) and catalase (CAT) were measured by
validated standard methods. In this study, while SOD activity was lower
(p<0,05) in the post-anesthezia; a statistically significant difference
wasn't determined between the levels of Cu, Zn, Mn and, CAT, GPx, MDA
(p>0.05). In conclusion, we regard to it was seen that sera copper, zinc,
manganese levels and oxidative stress parameters, ketamine-xylazine anesthesiz
can be used safely in Bozova greyhounds

Anahtar Kelimeler

Bozova Greyhounds, oxidative stress, SOD, CAT, GPx, MDA, Cu, Zn

Kaynakça

• Abdel-Hafez HZ, Mohamed EE, Abd-Elghany AA. (2010): Tissue and blood superoxide dismutase activity and malondialdehyde level in leprosy. J Eur Acad Dermatol Venereol. 24(6):704-8.
• Aebi HE (1983): Catalase in: H.U.Bermeyer (Hrsy). Methods of enzymatic analysis. Verlag Chemie; Weinheim, Bd. III, 273-286.
• Allen DG, Dyson DH, Pascoe PJ, O'Grady MR (1986): Evaluation of a xylazine-ketamine hydrochloride combination in the cat. Can J Vet Res. Jan; 50(1): 23–26.
• Altınışık M (2015) Serbest Radikaller. Erişim: http://www.mustafaaltinisik.org.uk/21-adsem-01b.pdf. Erişim Tarihi: 03.03.2015.
• Altuğ ME, Gönenci R, Yarsan E, Özturk A (2008): Effect of Induction Agents on the Antioxidative Activity of Desflurane in Dogs. YYÜ VET FAK DERG 19(1): 29-33.
• Andriichuk A, Tkachenko H, Tkachova I (2015): Oxidative Stress Biomarkers and Erythrocytes Hemolysis in Well-Trained Equine Athletes before and after Exercise. http://dx.doi.org/10.1016/j.jevs.2015.09.011Published Online: October 08, 2015. Publication stage: In Press Accepted Manuscript.
• Buege AJ, Aust SD (1978): Microsomal Lipid Peroxidation. Methods in Enzymology 52:302-10.
• Bickers DR, Athar M (2006): Oxidative stress in the pathogenesis of skin disease. J Invest Dermatol 126(12):2565-75.
• Camkerten I, Sahin T, Borazan G, Gökçen A, Erel O, Das A (2009): Evaluation of blood oxidant/antioxidant balance in dogs with sarcoptic mange. Veterinary Parasitology 161: 106–109.
• Haskins SC, Farver TB, Patz JD (1985): Ketamine in dogs. Am J Vet Res 46(9): 1855-60
• Haskins SC, Patz JD, Farver TB (1986): Xylazine and xylazine-ketamine in dogs. Am J Vet Res 47, 636- 641
• Klide AM, Calderwood HW, Soma LR (1975): Cardiopulmonary effects of xylazine in dogs. Am J Vet Res 36: 931-935.
• Kurtdede A, Börkü MK, Özlem MB, Kalınbacak A (1994): Sağlıklı köpeklerde xylazine ve xylazine+ketamine ‘nin kan gazları ve bazı hematolojik parametreler üzerindeki etkileri. A.Ü. Vet. Fak. Derg 41(3,4): 327-335.
• Lee JY, Kim MC (2012): Effect of propofol on oxidative stress status in erythrocytes from dogs under general anaesthesia. Acta Veterinaria Scandinavica 54(1):76.
• Paglie DE, Valantine WN (1967): Studies on Qualitative and Quantative Characterization of Eeythrocyte Glutathion Peroxidase. J Lab Clin Met 70: 158-169.
• Sun Y, Oberley LW, Li Y (1988): A simple for clinical assay of superoxide dismutase. Clin Chem 34: 497-500.
• Şındak N, Camkerten İ, Ceylan C (2010): Clinical Evaluation of Ketamine-Xylazine Anesthesia in Bozova Greyhounds. Journal of Animal and Veterinary Advances 9 (15): 2025-2029.
• Tietz WN (1987): Measurement of plasma hemoglobin. Fundamental of Clinical Chemistry. Saunders Company. 805-806.
• Todorova I, Simeonova G, Kyuchukova D, Dinev D, Gadjeva V (2005): Reference values of oxidative stress parameters (MDA, SOD, CAT) in dogs and cats. Comp Clin Path 13: 190
• Yalcin S, Aydogan H, Yuce HH, Kucuk A, Karahan Mahmut Alp, Vural M, Camuzcuoglu A, Aksoy N (2013): Effects of sevoflurane and desflurane on oxidative stress during general anesthesia for elective cesarean section. Wien Klin Wochenschr 125:467–473.
• Yarsan E, Gurkan M, Pekcan Z, Ince S, Kumandas A (2010): Effects of Halothane and Isoflurane Anaesthesia on Antioxidant Enzymes in Dogs Journal of Animal and Veterinary Advances 9 (19): 2513-2516.
• Wagner AE, Muir WW, Hinchdiff KW (1991): Cardiovascular effects of xylazine and detomidine in horses. Am J Vet Res 52: 651-657.
• Winterbourn CC, Hawkins RE, Brain M, Carrel W (1975): The estimation of red cell superoxide dismutase activity. J Lab Clin Med, 55, 337-341.