Wound healing effects of bee venom on adipose tissue derived mesenchymal stem cells

Yıl 2023, Cilt: 94 Sayı: 1, 59 - 66, 15.01.2023

Özge Özgenç , Sedat Sevin

https://doi.org/10.33188/vetheder.1183380

Cited By: 2

Öz

The objective of this study is to determine the effects of bee venom on the proliferation capacity of mesenchymal stem cells and wound healing. For this purpose, mesenchymal stem cells were isolated from canine adipose tissue and bee venom samples were collected from Apis mellifera anatoliaca in Muğla province of Türkiye. Cell viability test was performed on mesenchymal stem cells exposed to various concentrations (40 ppm, 20 ppm, 10 ppm, 5 ppm, 2.5 ppm, 1.25 ppm, 0.625 ppm and 0.312 ppm) of bee venom. And wound healing test was performed on cells treated with the doses (5 ppm, 2.5 ppm, 1.25 ppm, 0.625 ppm) and imaged every two hours for 16 hours. According to the results of our study's cell proliferation assay and wound healing test, bee venom had no proliferative effect on mesenchymal stem cells within the defined dose range. The study's outcomes may be enhanced by investigating the effect of bee venom on mesenchymal stem cells in combination with other substances or by improving the bee venom's purification process. Even while we have a better understanding of the mechanisms of action of bee venom components, there are still a lot of unanswered questions on the subject. It is believed that figuring out how bee venom affects wound healing may be useful for advancing wound care in both veterinary and human medicine.

Anahtar Kelimeler

honey bee, bee venom, mesenchymal stem cell

Arı zehirinin yağ dokusu kaynaklı mezenkimal kök hücre üzerindeki yara iyileştirici etkileri

Öz

Bu çalışmanın amacı, arı zehirinin mezenkimal kök hücrelerin çoğalma kapasitesi ve yara iyileşmesi üzerindeki etkilerini belirlemektir. Bu amaçla köpek yağ dokusundan mezenkimal kök hücreler izole edilmiş ve Muğla çevresinde bulunan Apis mellifera anatoliaca'dan arı zehiri örnekleri toplanmıştır. Farklı konsantrasyonlarda arı zehirine maruz bırakılan mezenkimal kök hücreler üzerinde hücre canlılığı testi (40 ppm, 20 ppm, 10 ppm, 5 ppm, 2.5 ppm, 1.25 ppm, 0,625 ppm ve 0.312 ppm) yapılmıştır. Hücreler üzerinde 5 ppm, 2.5 ppm, 1.25 ppm, 0.625 ppm arı zehri kullanılarak yara iyileştirme deneyi yapılmış ve 16 saat boyunca her iki saatte bir görüntülenmiştir. Çalışmanın hücre canlılığı ve yara iyileşme deneylerinin sonuçlarına göre, arı zehirinin köpek yağ dokusu kaynaklı mezenkimal kök hücreler üzerinde tanımlanan doz aralığında proliferatif bir etkisi olmamıştır. Çalışmanın sonuçları, diğer maddelerle birlikte mezenkimal kök hücreler üzerindeki arı zehirinin etkisini araştırarak veya arı zehirinin saflaştırma sürecini geliştirerek iyileştirilebilir. Arı zehiri bileşenlerinin etki mekanizmalarını daha iyi anlamış olsak da konuyla ilgili hala cevaplanmamış birçok soru bulunmaktadır. Arı zehrinin yara iyileşmesi üzerindeki etkisinin belirlenmesinin hem veteriner hekimlikte hem de beşeri hekimlikte yara tedavisini kolaylaştırmada avantaj sağlayacağı düşünülmektedir.

Anahtar Kelimeler

bal arısı, arı zehri, mezenkimal kök hücre

Kaynakça

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