Cytotoxic and Cytogenetic Effects of Bisphenol-A in Mytilus Galloprovincialis (Lamarck 1819)

Yıl 2017, Cilt: 17 Sayı: 1, 93 - 101, 27.02.2017

Özlem Çakal Arslan , Hatice Parlak , Muhammet Ali Karaaslan Meltem Boyacıoğlu

https://doi.org/10.17693/yunusae.v17i26557.282169

Öz

Bisphenol-A
(BPA), is one of the most important industrial chemicals synthesized for
diverse applications. The environmental concentrations of BPA are at high risk
level due to use widely in many fields of industry according to the latest
studies. However, less knowledge has been founded to the
toxicity of BPA. No studies have been founded genotoxicity of BPA to the Mytilus galloprovincialis (Lamarck 1819). The Micronucleus test is a biomarker of
mutagenicity/genotoxicty used for determining changes in DNA fragments exposure
to pollution and chemicals. For this purpose, frequency of MN and other nuclear
abnormalities (binucleated, nuclear buds, fragmented/apoptotic cells) was
analyzed. Increasing concentrations of BPA (12.5-100 µg-BPA/L) were tested for
determination of MN under semi-static conditions in the laboratory. According
to the results of the study, it is revealed that all concentration of BPA
increases the genotoxic damage, and elevates the micronucleus frequency from ‰
11, 33 to ‰ 37, 35 Thus, it was tried to determine whether BPA affected the
cells of M. galloprovincialis (Lamarck 1819) at the level of DNA through to the MN assay. 

Anahtar Kelimeler

Micronucleus Assay, Bisphenol-a, Mytilus galloprovincialis, Genotoxicity

Bisfenol- A’ nın Mytilus galloprovincialis (Lamarck 1819) Üzerine Sitotoksik ve Genotoksik Etkileri

Öz

Bisfenol-A (BPA), çeşitli uygulamalar için
sentezlenen önemli endüstriyel kimyasallardan biridir. En son çalışmalara göre
sanayinin birçok alanında yaygın kullanımı nedeniyle BPA’ nın çevresel
konsantrasyonları yüksek risk düzeyinde bulunmaktadır. BPA’ nın Mytilus galloprovincialis (Lamarck 1819) üzerine genotoksisitesinin belirlendiği çalışma
bulunmamaktadır. Mikronukleus
denemeleri, interfaz hücrelerin sitoplazmasında ki DNA parçacıklarının
değişimine neden olan kimyasalların ve kirliliğin belirlenmesinde kullanılan
mutajenite test sistemidir. Bu amaçla, micronukleus (MN) ve diğer çekirdek
anormallikleri (nuklear bud, binukleus, apoptatcik çekirdek) incelenmiştir.
Midyeler BPA’ nın (12.5-100 µg-BPA/L) artan konsantrasyonları
semi-statik labaratuvar şartlarında maruz bırakılmışlardır. Çalışmanın
sonuçlarına göre; BPA’ nın tüm konsantrasyonları genotoxic hasarı arttırmakta
ve ‰ 11, 33 to ‰ 37, 35 MN oluşumuna neden olduğu gözlenmiştir. Bu nedenle, MN
testi ile BPA’ nın DNA düzeyinde, M. galloprovincialis'
in hücreleri etkileyip etkilemediğini belirlenmeye çalışılmıştır.

Anahtar Kelimeler

Micronukleus Testi, Bisphenol-a, Mytilus galloprovincialis, Genotoksisite

Kaynakça

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