Kozluk (Batman) Tuzla Gözü Tuzlasından İzole edilen Ektraselüler İnulinaz Enzimi Üreten Salinivibrio T1 İzolatının Karakterizasyo

Yıl 2018, Cilt: 23 Sayı: 1, 60 - 68, 27.04.2018

Kerem Özdemir Erdal Öğün Metin Ertaş Necati Özok

Öz

Bu araştırmanın materyalini; Güneydoğu Anadolu bölgesinde, Kozluk (Batman) Tuzla Gözü tuzlasından toplanan tuzlu toprak örnekleri oluşturdu. Tuzlu toprak örneklerinden izole edilen, Salinivibrio T1 izolatının, inulinaz enzimi üretimi yönünden pozitif olduğu tespit edildi. Bu izolatın inulinaz enzimi üretme yeteneği, içerisinde %1 oranında inulin bulunan halofil agarda test edildi. Salinivibrio T1 izolatının, morfolojik, fizyolojik ve biyokimyasal özelliklerine ve 16S rDNA gen bölgesinin diziliş analizine göre Salinivibrio costicola türüne ait olduğu belirlendi.

Anahtar Kelimeler

İnulinaz, Halofilik, Salinivirio, Tuzla Gözü

Kaynakça

• Aşan Özüsağlam, M., 2009. İnulinaz Enziminin Önemi. Anadolu Üniversitesi Bilim ve Teknoloji Dergisi 10;2: 327-334. Babavalian, H., Amoozegar, M.A., Pourbabaee, A.A., Moghaddam, M.M., Shakeri, F., 2013. Isolation and identification of moderately halophilic bacteria producing hydrolytic enzymes from the largest hypersaline playa in Iran. Microbiology, 82;4: 466-474. Babavalian, H., Amoozegar, M.A., Zahraei, S., Rohban, R., Shakeri, F., Moghaddam, M.M., 2014. Comparison of bacterial biodiversity and enzyme production in three hypersaline lakes; Urmia, Howz-Soltan and Aran-Bidgol. Indian journal of microbiology, 54;4: 444-449. Biswas, J., Paul, A.K., 2013. Production of Extracellular Enzymes by Halophilic Bacteria Isolated from Solar Salterns. International Journal of Applied Biology and Pharmaceutical Technology, 4;4: 30-36. Canli, O., Kurbanoglu, E.B., 2012. Application of low magnetic field on inulinase production by Geotrichum candidum under solid state fermentation using leek as substrate. Toxicology and industrial health, 28;10: 894-900. Collins, C.H., L, P.M, Grange J.M., Falkinham J.O., 2004. Collins and Lyne’s Microbiological methods., Eighth Edition, Arnold, London. Çınar, S., Mutlu, M. B., 2017. Sivas’ın Solar Tuzlalarındaki Mikrobiyal Toplulukların Kültür-Bağımlı Karakterizasyonu. Anadolu Üniversitesi Bilim ve Teknoloji Dergisi-C Yaşam Bilimleri ve Biyoteknoloji, 1-1. DasSarma, S. , Arora, P., 2001. Halophiles. Encyclopedia of Life Sciences, 1-9. Delgado-García, M., Valdivia-Urdiales, B., Aguilar-González, C. N., Contreras-Esquivel, J. C., Rodríguez-Herrera, R., 2012. Halophilic hydrolases as a new tool for the biotechnological industries. Journal of the Science of Food and Agriculture, 92;13: 2575-2580. Empadinhas, N., da Costa, M.S., 2008. Osmoadaptation mechanisms in prokaryotes: distribution of compatible solutes. International Microbiology 11;3: 151-61. Erdal, S., Canli, O., Algur, O.F. 2011. Inulinase production by Geotrichum candidum using Jerusalem artichoke as sole carbon source. Romanian Biotechnological Letters, 16;4: 6375-6382. Ertan, F., Aktaç, T., Kaboglu, C., Ekinci, F., Bakar, E., 2003a. Determination of optimum cultivation conditions on the production of inulinase from Rhizoctonia solani. Pakistan Journal of Biological Sciences, 6;16: 1386-1388. Ertan, F., Ekinci, F., Aktaç, T., 2003b. Production of inulinases from Penicillium spinulosum, Aspergillus parasiticus NRRL 2999 and Trichoderma viride. Pakistan Journal of Biological Sciences 6;15: 1332-1335. George, M.G., Julia, A.B., Timothy, G.L., 2005. Bergey’s manual of systematic bacteriology. In Volume 2: The Proteobacteria (pp. 552-556). Springer-Verlag, Berlin. Gomaa, O.M., Momtaz, O.A., 2007. 16S rRNA characterization of a Bacillus isolate and its tolerance profile after subsequent subculturing. Arab Journal of Biotechnology, 10: 107-116. Gong, Y., Yu, J., Liu, Q., Chen, J., Yu, C., 2014. Research advance on inulinase produced by microorganism. Journal of Food Safety and Quality, 5;7: 2146-2150. Jayachandra, S.Y., Parameshwar, A.B., Mohan, R.K., Sulochana, M.B., 2012a. Characterization of extracellular hydrolytic enzymes producing extremely halophilic bacterium Virgibacillus sp. World Journal of Science and Technology, 2;2: 23-26. Jayachandra, S.Y., Kumar, A., Merley, D.P., Sulochana, M.B., 2012b. Isolation and characterization of extreme halophilic bacterium Salinicoccus sp. JAS4 producing extracellular hydrolytic enzymes. Recent Research in Science and Technology, 4;4: 46-49. Jukes T.H., Cantor C.R., 1969. Evolution of protein molecules. In Munro HN, editor, Mammalian Protein Metabolism, pp. 21-132, Academic Press, New York. Karatop, R., Sanal, F., 2013. A Potential Resource in Fructose Production from Inulin: Aspergillus wentii Inulinase. Journal of Cell and Molecular Biology, 11;1/2: 21. Kumar, S., Nei, M., Dudley, J., Tamura K., 2008. MEGA: A biologist-centric software fr evolutionary analysis of DNA and protein sequences. Briefing in Bioinformatics, 9;4: 299-306. Kumar, S.A., Arunasri, R., Jayachandra, Y., Sulochana, M.B., 2010. Screening Of Extracellular Hydrolytic Enzymes From Marinobacter hydrocarbonoclasticus Strain AK 5. Bioscan, 5;1: 97-99. Lanyi, J.K., 1974. Salt-dependent properties of proteins from extremely halophilic bacteria. Bacteriological Reviews, 38;3: 272. Li, A.X., Guo, L.Z., Fu, Q., Lu, W.D., 2011. A simple and rapid plate assay for screening of inulindegrading microorganisms using Lugol’s iodine solution. African Journal of Biotechnology, 10;46: 9518-9521. Li, A.X., Guo, L.Z., Lu, W.D., 2012. Alkaline inulinase production by a newly isolated bacterium Marinimicrobium sp. LS–A18 and inulin hydrolysis by the enzyme. World Journal of Microbiology and Biotechnology, 28;1: 81-89. Lu, W.D., Li, A.X., Guo, Q.L., 2014. Production of novel alkalitolerant and thermostable inulinase from marine actinomycete Nocardiopsis sp. DN-K15 and inulin hydrolysis by the enzyme. Annals of Microbiology, 64;2: 441-449. Madern, D., Ebel, C., Zaccai, G., 2000. Halophilic adaptation of enzymes. Extremophiles, 4;2: 91-98. Meenakshi, S., Umayaparvathi, S., Manivasagan, P., Arumugam, M., Balasubramanian, T., 2013. Purification and characterization of Inulinase from marine bacterium, Bacillus cereus MU-31. Indian Journal of Geo-Marine Sciences, 42;4: 510-515 Mellado, E., Moore, E.R.B., Nieto, J.J., Ventosa, A., 1996. Analysis of 16S rRNA gene sequences of Vibrio costicola strains: description of Salinivibrio costicola gen. nov., comb. nov. International Journal of Systematic and Evolutionary Microbiology, 46;3: 817-821. Nikiforov, Y.E., Howles, P.N., 2001. Polymerase chain reaction. In Morphology Methods (pp. 181-207). Humana Press. Oren, A., 2008. Microbial life at high salt concentrations: phylogenetic and metabolic diversity. Saline systems, 4;1: 2. Rawat, H.K., Soni, H., Treichel, H., Kango, N.. 2017. Biotechnological potential of microbial inulinases: recent perspective. Critical reviews in food science and nutrition, 57;18: 3818–3829 Roberts, M.F., 2005. Organic compatible solutes of halotolerant and halophilic microorganisms. Saline Systems, 1;5: 1-30. Rohban, R., Amoozegar, M.A., Ventosa, A., 2009. Screening and isolation of halophilic bacteria producing extracellular hydrolyses from Howz Soltan Lake, Iran. Journal of industrial microbiology & biotechnology, 36;3: 333-340. Saeed, M., Yasmin, I., Pasha, I., Randhawa, M.A., Khan, M.I., Shabbir, M.A., Khan, W.A., 2015. Potential application of inulin in food industry: A review. Pakistan Journal of Food Sciences, 25;3: 110-116. Schneegurt, M. A. (2012). Media and conditions for the growth of halophilic and halotolerant bacteria and archaea. In Advances in Understanding the Biology of Halophilic Microorganisms (pp. 35-58). Springer, Dordrecht. Schneider, A.L., Gern, R.M., Ninow, J.L., Furlan, S.A., Jonas, R., 2004. Characterization Of Microbial Inulinases. In: Biotechnological Advances and Applications in Bioconversion of Renewable Raw Materials. GBF and InWEnt, Germany. ISBN ISBN 3-925 268-25-0 Shen, J., Zhang, R., Li, J., Tang, X., Li, R., Wang, M., Zhou, J., 2015. Characterization of an exo-inulinase from Arthrobacter: a novel NaCl-tolerant exo-inulinase with high molecular mass. Bioengineered, 6;2: 99-105. Tarhriz, V., Mohammadzadeh, F., Hejazi, M.S., Nematzadeh, G., Rahimi, E., 2011. Isolation and characterization of some aquatic bacteria from Qurugol Lake in Azerbaijan under aerobic conditions. Advances in Environmental Biology, 3173-3179. Tasar, O.C., Erdal, S., Algur, O.F, 2015. Utilization of Leek (Allium ampeloprasum var. porrum) for Inulinase Production. Preparative Biochemistry and Biotechnology, 45;6: 596-604. William, S., Feil, H., Copeland, A., 2012. Bacterial genomic DNA isolation using CTAB. Sigma, 50; 6876. Yabancı, N., 2010. İnülin ve Oligofruktozların İnsan Sağlığı ve Beslenmesi Üzerine Etkileri. Akademik Gıda, 8;1: 49-54. Yin, J., Chen, J.C., Wu, Q., Chen, G.Q., 2015. Halophiles, coming stars for industrial biotechnology. Biotechnology Advances, 33;7:1433-1442.