Molecular Characterization of Zenit×B27 Durum Wheat Population Using SSR Markers

Yıl 2023, Sayı: 378, 78 - 86, 30.12.2023

İlker Yüce , Hatice Osanmaz , Ziya Dumlupınar

https://doi.org/10.33724/zm.1375967

Öz

In this study, 11 durum wheat genotypes obtained from Zenit and B27 parents used to determine some disease and quality related alleles using eight DNA markers durum wheat genotypes. As a result of molecular screening, 8 DNA markers produced 25 alleles and the average polymorphism information content (PIC) value of the DNA markers used in the study was 0.9775. The highest polymorphism information content value was 0.99, while the lowest polymorphism information content value was 0.95. In the dendrogram created by using 25 alleles produced by DNA markers in the study, two main groups were formed according to the parents. Zenit×B27-7, Zenit×B27-9 and Zenit×B27-11 and Zenit×B27-5, Zenit×B27-6, Zenit×B27-8 and Zenit×B27-10 cross combinations were found 100% similar. According to the results of the research, genes belonging to Waxy (Wx-A1) trait were determined in Zenit×B27_1, Zenit×B27_2 and Zenit×B27_3 genotypes, while the gene region belonging to high protein (Gpc-B1) was found in Zenit×B27_3 and Zenit×B27_5 genotypes.

Anahtar Kelimeler

Durum wheat, SSR, DNA marker

Zenit×B27 Makarnalık Buğday Popülasyonunun SSR Markörleriyle Moleküler Karakterizasyonu

Öz

Araştırmada, Zenit ile B27 yerel makarnalık buğday çeşidi ve bu çeşitlerin melezlenmesi sonucunda elde edilen 11 adet makarnalık buğday genotipi, 8 adet allel spesifik DNA markörü kullanılarak bazı hastalık ve kalite ile ilgili allellerin tespiti yapılmıştır. Moleküler tarama sonucunda kullanılan 8 DNA markörü 25 adet allel üretirken, çalışmada kullanılan DNA markörlerinin ortalama polimorfizm bilgi içeriği (PIC) değeri 0.9775 olarak tespit edilmiştir. En yüksek polimorfizm bilgi içeriği değeri 0.99 olarak hesaplanırken, en düşük polimorfizm bilgi içeriği değeri 0.95 olarak hesaplanmıştır. Çalışmada DNA markörleri tarafından üretilen 25 allel kullanılarak oluşturulan dendrogramda, ebeveynlere göre iki ana grup meydana gelmiştir. Zenit×B27-7, Zenit×B27-9 ve Zenit×B27-11 ile Zenit×B27-5, Zenit×B27-6, Zenit×B27-8, Zenit×B27-10 melez kombinasyonları % 100 benzer bulunmuştur. Araştırma sonuçlarına göre Zenit×B27-1, Zenit×B27-2 ve Zenit×B27-3 genotiplerinde Waxy (Wx-A1) özelliğine ait genler belirlenirken, Zenit×B27-3 ve Zenit×B27-5 genotiplerinde yüksek proteine (Gpc-B1) ait gen bölgesinin yer aldığı saptanmıştır.

Anahtar Kelimeler

Makarnalık buğday, SSR, DNA markörü

Kaynakça

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