Aspartam ve asesülfam K kullanımının testis yapısına etkilerinin ince yapı düzeyinde incelenmesi

Year 2020, Volume: 6 Issue: 3, 320 - 331, 03.12.2020

Leman Sencar , Yurdun Kuyucu

https://doi.org/10.30569/adiyamansaglik.789927

Abstract

Amaç: Bu çalışma, ülkemizde ve dünyada yaygın olarak kullanılan aspartam ve asesülfam K’nın, sıçanlarda testis dokuları üzerindeki etkilerinin ışık ve elektron mikroskobik düzeyde araştırılması amacıyla yapılmıştır.
Gereç ve Yöntem: Çalışmada 60 adet erkek sıçan kullanıldı ve bu denekler rastgele olarak 6 eşit gruba ayrıldı. 1. gruptaki hayvanlar kontrol grubu olarak değerlendirildi; 2.gruptaki hayvanlara 200 mg/kg/gün aspartam; 3.gruptaki hayvanlara 300 mg/kg/gün aspartam; 4. gruptaki hayvanlara 300 mg/kg/gün asesülfam K; 5. gruptaki hayvanlara 600 mg/kg/gün asesülfam K ve son gruba da 300 mg/kg/gün aspartam+300 mg/kg/gün asesülfam K birlikte 8 hafta süreyle verildi. 8. haftanın sonunda deneklerden testis dokuları alındı ve dokular ışık ve elektron mikroskobik olarak değerlendirildi.
Bulgular: Aspartam ve asesülfam K kullanımının testis dokusunda dejenerasyona yol açtığı ve spermatogenez sürecinde sağlıklı spermatid oluşumunu engellediği izlendi.
Sonuç: Aspartam ve asesülfam K tüketiminin erkek sıçanlarda testis dejenerasyonuna neden olduğu görüldü. Bununla birlikte, erkek üreme sistemindeki aspartam ve asesülfam K toksisitesinin doğrulanması için daha kapsamlı çalışmaların yapılması gerektiği kanaatine varıldı.

Keywords

Asesülfam K, Aspartam, Elektron Mikroskop, Işık Mikroskop, Testis

Ultrastructural examination of the effects aspartame and acesulfame K on testis structure

Abstract

Aim: The present study was carried out to investigate the effects of aspartame and acesulfame K, which are widely used in our country and the world, on testicular tissues in rats at light and electron microscopic level.
Materials and Methods: In this experimental study, 60 male rats were used. The experimental animals were randomly divided into 6 equal groups. Animals in group 1 were considered as control group; Animals in group 2 received 200 mg/kg/day of aspartame; Group 3 animals received 300 mg/kg/day aspartame; Animals in group 4 received acesulfame K at 300 mg kg/day; The animals in the 5th group were given acesulfame K 600 mg/kg/day and the last group received 300 mg/kg/day aspartame+300 mg/kg/day acesulfame K for 8 weeks. At the end of the 8th week, testicular tissue samples obtained from rats were examined by light and electron microscopic methods.
Results: It has been observed that the use of aspartame and acesulfame K causes degeneration in the testicular tissue depending on the döşe and it has been observed that prevents the formation of healthy spermatids during the spermatogenesis process.
Conclusion: The findings of the present study elucidated that consumption of aspartame and acesulfame K resulted in testis damages in mice. Confirmation of the toxicity of aspartame in male reproductive system requires more extensive experimental studies and clinical trials.

Keywords

Acesulfame K, Aspartame, Electron microscope, Light microscope, Testis

References

• Solomi L, Rees GA, Redfern KM.The acute effects of the non-nutritive sweeteners aspartame and acesulfame-K in UK diet cola on glycaemic response. International Journal of Food Sciences and Nutrition. 2019;70(7): 894–900.
• Ibi D, Suzuki F, Hiramatsu M. Effect of AceK (acesulfame potassium) on brain function under dietary restriction in mice. Physiology & Behavior. 2018; 188:291–97.
• Marinovich M, Galli CL, Bosetti C, Gallus S, La Vecchia C. Aspartame, low-caloriesweeteners and disease: regulatorysafety and epidemiological issues. Food and Chemical Toxicology. 2013;60:109-15.
• Yılmaz S, Uçar A. A review of the genotoxic and carcinogenic effects of aspartame: does it safe or not? Cytotechnology. 2014;66:875-81.
• Ardalan MR, Tabibi H, Attari VE, Mahdavi AM. Nephrotoxic Effect of Aspartame as an Artificial Sweetener. Iranian Journal of Kidney Diseases. 2017;11(5): 339-43.
• Alkafafy ME, Ibrahim ZS, Ahmed M, El-Shazly SA. Impact of aspartame and saccharin on the rat liver: Biochemical, molecular, and histological approach. International Journal of Immunopathology and Pharmacology. 2015;28(2):247–55.
• Ashok I, Poornima PS, Wankhar D, Ravindran R, Sheeladevi R. Oxidative stress evoked damages on rat sperm and attenuated antioxidant status on consumption of aspartame. International Journal of Impotence Research. 2017;29:164–70.
• Mukhopadhyay M, Mukherjee A, Chakrabartı J. In Vivo Cytogenetic Studies on Blends of Aspartame and Acesulfame-K. Food and Chemical Toxicology.1998;38(2000):75-7.
• Anbara H, Sheibani MT, Razi M. Long-Term Effect of Aspartame on Male Reproductive System: Evidence for Testicular Histomorphometrics, Hsp70-2 Protein Expression and Biochemical Status. International Journal of Fertility and Sterility. 2020;14(2):91-101.
• Hassen EZ, Mahmoud AA, Ibrahem NE, El-Shal AS. The Effect of Long Term Administration of Aspartame on the Sciatic nerve of adult male albino rats and the Possible Therapeutic Role of Ozone (Histological and Biochemical Study). The Egyptian Journal of Histology. 2019;42(1):191-201.
• Mallikarjun S, Sieburth RM. Aspartame and Risk of Cancer: A Meta-analytic Review. Archives of Environmental & Occupational Health. 2013;70(3):133-41.
• Shalabya AM, Ibrahima MAAH, Aboregela AM. Effect of aspartame on the placenta of adult albino rat. A histological and immunohistochemical study. Annals of Anatomy. 2019;224: 133–41.
• Potenza DP, El-Mallakh RS. Aspartame-Clinical update. Connecticut Medicine. 1989; 53:395-400.
• Baydar T, Şahin G. Aspartam Metabolizması ve Toksisitesi. Türkiye Klinikleri Tıp Bilimleri Dergisi. 1997;17(3):141-52.
• Oyama Y, Sakai H, Arata T, Okano Y, Akaike N, Sakai K, et al. Cytotoxic effects of methanol, formaldehyde, and formate on dissociated rat thymocytes: a possibility of aspartame toxicity. Cell Biology Toxicology. 2002;18(1):43-50.
• Trocho C, Pardo R, Rafecas I, Virgili J, Remesar X, Fernandez-Lopez JA, et al. Formaldehyde derived from dietary aspartame binds to tissue components in vivo. Life Sciences. 1998; 63(5): 337-49.
• Ashok I, Poornima PS, Wankhar D, Ravindran R, Sheeladevi R. Oxidative stress evoked damages on rat sperm and attenuated antioxidant status on consumption of aspartame. International Journal of Impotence Research. 2017; 29(4): 164-70.
• El-Ezaby MM, Hamide NA, Marwa AE, El-Maksoud A, Shaheen EM, Embashi MR. Effect of some food additives on lipid profile, kidney function and liver function of adult male albino rats. Journal of Basic and Environmental Sciences. 2018;5:52-9.
• Choudhary AK, Devi RS. Effects of aspartame on hsp70, bcl-2 and bax expression in immune organs of Wistar albino rats. The Journal of Biomedical Research. 2016;30(5):427–35.
• Gong T, Wei Q, Mao D, Nagaoka K, Watanabe G, Taya K, Shi F. Effects of Daily Exposure to Saccharin and Sucrose on Testicular Biologic Functions in Mice. Biology of Reproductıon. 2016; 95(6): 1–13.
• Asri-Rezaei S, Nourian A, Shalizar-Jalali A, Najafi G, Nazarizadeh A, Koohestani M, et al. Selenium supplementation in the form of selenium nanoparticles and selenite sodium improves mature male mice reproductive performances. Iran Journal of Basic Medical Sciences. 2018;21(6):577-85.
• Anbara H, Shahrooz R, Razi M, Malekinejad H, Najafi G. The effect of vitamin C on mice hemolytic anemia induced by phenylhydrazine: an animal model study using histological changes in testis, preimplantation embryo development, and biochemical changes. Iran Journal of Basic Medicine Sciences. 2018; 21(7):668-77