Abiyotik stres koşullarında yetiştirilen Gypsophila pilulifera (Caryophyllaceae) tohumlarının in vitro çimlenme ve vejetatif büyüme özellikleri

Year 2021, Volume: 5 Issue: 2, 91 - 97, 15.11.2021

Hatice Üstüner , Ayşe Gül Nasırcılar , Mustafa Yavuz , Ramazan Süleyman Göktürk

https://doi.org/10.30616/ajb.935421

Cited By: 2

Abstract

Gypsophila pilulifera Boiss. & Heldr. Türkiye'de ekonomik açıdan önemli, nesli tükenmekte olan (CR) endemik bir türdür. Bu tür bir Akdeniz elementidir ve tip örneği C3 karesinde (Antalya) bulunmaktadır. Bitkiler, özellikle çimlenme ve fide gelişme aşamalarında abiyotik stres faktörlerine çok daha duyarlıdır. Bu çalışmada in vitro koşullarda G. pilulifera Boiss. & Heldr. (Caryophyllaceae) 'in tuz ve kuraklık stresi altında çimlenme ve fide büyüme özellikleri incelendi. Tohumlar, artan NaCl (50, 100, 150, 200 mM) ve PEG 6000 (%5, %10, %15 ve %20) dozlarını içeren hormonsuz MS ortamında kültüre alındı. Çimlenen tohumlar ilk hafta üç günde bir ve ikinci hafta için her gün sayıldı. Bitkilerin kök ve hipokotil uzunluğu, yaprak sayısı, taze ağırlık ve canlılık indeksi 21 gün sonunda belirlendi. Artan tuz ve kuraklık stresi ile incelenen tüm bitkisel büyüme parametreleri arasında negatif bir korelasyon belirlendi. Ayrıca kuraklık oranı en yüksek olan MS besiyerinde (%20) çimlenme elde edilmesine rağmen 200 mM NaCl içeren MS besiyerinde çimlenme elde edilemedi. Sonuç olarak bitkinin tuzluluk stresine kuraklığa göre daha duyarlı olduğu ve yüksek tuz konsantrasyonlarının çimlenmeyi %100 engellediği belirlenmiştir.

Keywords

Gypsophila pilulifera, kuraklık, tuzluluk, vejetatif büyüme, vigor indeks

In vitro germination and vegetative growth characteristics of Gypsophila pilulifera (Caryophyllaceae) seeds grown under abiotic stress conditions

Abstract

Gypsophila pilulifera Boiss. & Heldr. is an economically important critically endangered (CR) endemic species of Turkey. This species is a Mediterranean element and type sample is located in square C3 (Antalya). Plants are much more sensitive to abiotic stress factors, especially during germination and seedling development stages. In this study, in vitro germination and seedling growth properties of G. pilulifera Boiss. & Heldr. (Caryophyllaceae) were investigated under salt and drought stress. The seeds were cultured in hormone-free MS media containing increasing doses of NaCl (50, 100, 150, 200 mM) and PEG 6000 (5%, 10%, 15% and 20%). Germinated seeds were counted every three days for the first week and every day for the second week. The root and hypocotyl length, number of leaves, fresh weight and vigor index of the plants were determined at the end of 21 days. A negative correlation was determined between increased salt and drought stress and all vegetative growth parameters. Although germination was obtained on MS medium with the highest level of drought (20%), there was no germination on MS medium containing 200 mM NaCl. As a result, it was determined that the plant was more sensitive to salinity stress than drought and high salt concentrations inhibited the germination by 100%.

Keywords

Gypsophila pilulifera, Drought, Salinity, Vegetative growth, Vigor index

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