Interactive effect of thiourea application on morphological and physiological characteristics in Cicer arietinum L. grown at different temperatures

Year 2022, Volume: 6 Issue: 2, 83 - 91, 15.11.2022

Sema Leblebici , Fadime Donbaloğlu Bozca

https://doi.org/10.30616/ajb.1139277

Abstract

Global warming affects many metabolic events in plants and significantly reduces yield and product quality. One of the physiological events most affected by heat stress is nitrogen metabolism. In this study, 5 and 10 mM thiourea was applied to chickpea plants grown at 15, 25, and 35 °C and it was aimed to determine how the plant can cope with heat stress with nitrogen supplementation. It was determined that the root length decreased significantly at all three temperatures depending on the increasing thiourea concentration, while the shoot length increased at 15 and 35 °C compared to the control. There was a decrease in root fresh weight in all three experimental groups due to increasing thiourea concentrations. Only at 5 mM at 15 °C was a highly significant increase seen over the control. When the experimental groups at all temperatures were compared, the highest chlorophyll a, b, and total chlorophyll values were found at 35 °C. It was determined that SOD activity decreased at all three temperatures compared to the control, while CAT and APX activity increased. A significant increase in NR and GS activity was determined in both thiourea treatments at 25 and 35 °C compared to the control.

Keywords

Antioxidant enzymes, chickpeas, heat stress, nitrogen metabolism, morphological parameters

Supporting Institution

Bilecik Şeyh Edebali Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

Project Number

2019-01.BŞEÜ.25-01

Thanks

This study was supported by Bilecik Şeyh Edebali University Scientific Research Projects Coordinatorship (Project number: 2019-01.BŞEÜ.25-01).

Farklı sıcaklıklarda yetiştirilen Cicer arietinum L.'de tiyüre uygulamasının morfolojik ve fizyolojik özellikler üzerine interaktif etkisi

Abstract

Küresel ısınma bitkilerde birçok metabolik olayı etkilemekte, verimi ve ürün kalitesini önemli ölçüde düşürmektedir. Sıcaklık stresinden en çok etkilenen fizyolojik olaylardan biri de azot metabolizmasıdır. Bu çalışmada, 15, 25 ve 35 °C'de yetiştirilen nohut bitkisine 5 ve 10 mM tiyoüre uygulanmış ve azot takviyesi ile bitkinin sıcaklık stresi ile nasıl başa çıkabileceğinin belirlenmesi amaçlanmıştır. Artan tiyoüre konsantrasyonuna bağlı olarak her üç sıcaklıkta da kök uzunluğunun önemli ölçüde azaldığı, sürgün uzunluğunun ise kontrole göre 15 ve 35 °C'de arttığı belirlendi. Artan tiyoüre konsantrasyonlarına bağlı olarak her üç deney grubunda da kök taze ağırlığında bir azalma olmuştur. 15 °C'de sadece 5 mM'de kontrol üzerinde oldukça önemli bir artış gözlenmiştir. Tüm sıcaklıklardaki deney grupları karşılaştırıldığında en yüksek klorofil a, b ve toplam klorofil değerleri 35 °C'de tespit edilmiştir. Her üç sıcaklıkta da kontrole göre SOD aktivitesinin azaldığı, CAT ve APX aktivitesinin ise arttığı belirlenmiştir. Kontrole kıyasla 25 ve 35°C'de her iki tiyoüre uygulamasında da NR ve GS aktivitesinde önemli bir artış tespit edilmiştir.

Keywords

antioksidan enzimler, nohut, sıcaklık stresi, nitrojen metabolizması, morfolojik parametreler

Project Number

2019-01.BŞEÜ.25-01

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