BibTex RIS Cite

-

Year 2014, Volume: 20 Issue: 3, 261 - 268, 14.08.2014

Mustafa İmren , Ece Börteçine Kasapoğlu Abdelfattah Dababat Halil Toktay İbrahim Halil Elekcioğlu

https://doi.org/10.15832/tbd.26896

Abstract

Cereal cyst nematodes (Heterodera avenae group) are important pests of wheat; they include the three most important species of cereal cyst nematodes: Heterodera avenae Wollenweber, H. latipons Franklin and H. filipjevi (Madzhidov) in Turkey that were determined in different wheat growing areas. It was reported that Mediterranean cereal cyst nematode, Heterodera latipons which is widely found in the centre of the durum wheat growing area -South-eastern Anatolia regionin our country was reaching the economical threshold in many locations. It is known that the use of resistant/tolerant wheat varieties is the most effective controlling method against cyst nematodes in the wheat cultivation. Nowadays, nine different resistance genes (Cre genes), which are transferred from wild wheat species to wheat cultivated forms are available. In this study, the efficiency of the resistance gene, Cre1, against the population of H. latipons from Gaziantep (Karkamıs) was investigated. In the study, 38 genotypes carrying Cre1 gene and 26 genomes lacking resistance genes; this gives a total 64 wheat genotypes which are produced by parental crossing of SILVERSTAR containing the resistance gene, Cre1, in its genome, SOKOLL, CALINGIRI, GOLDMARKER, CROC_1/AE.SQUARROSA(224)//OPATA, FRAME, STYLET and PASTOR were used against H. latipons in the experiment. Among the 64 genotypes; 20 were resistant, 32 were moderately resistant and 12 were moderately susceptible to the nematode. Ten resistant, 20 moderately resistant and 8 moderately susceptible lines were determined against H. latipons that these 38 lines carried the Cre1 gene in their genome. Within this study, the resistant gene, Cre1, was not found to be completely resistant against H. latipons, but its 78% efficiency rate was found to be successful: therefore, it was concluded that it can be used against H. latipons in national wheat breeding programs. Furthermore, PASTOR, SOKOLL and CROC_1/AE.SQUARROSA(224)//OPATA wheat genotypes were determined as good resistance source against H. latipons.

Keywords

Cereal cyst nematode; Heterodera latipons; Wheat; Resistance

References

  • Agrios G N (1997). Plant Pathology, fourth edition. Academic Press Inc., London
  • Akar T, Calıskan M, Nicol J M, Uranbey S, Sahin E, Yazar S, William M & Braun H J (2009). Molecular characterization of Cereal Cyst Nematode diagnostic markers Cre1 and Cre3 in some winter wheat germplasm and their potential use against Heterodera filipjevi, Field Crops Research 114(2): 320-323
  • Barloy D, Lemoine J, Abelard P, Tanguy A M, Rivoal R & Jahier J (2007). Marker-assisted Pyramiding of Two Cereal Cyst Nematode Resistance Genes From Aegilops variabilis in Wheat. Molecular Breeding 20: 31–40
  • Bekal S, Jahier J & Rivoal R (1998). Host response of different Triticeae to species of the cereal cyst nematode complex in relation to breeding resistant durum wheat. Fundamental Applied Nematology 21: 359-370
  • Boerma H R & Hussey R S (1992). Breeding Plants for Resistance to Nematodes. Journal of Nematology 24(2): 242–252
  • Cook R & Rivoal R (1998). Genetics of resistance and parasitism. In: Sharma, S. B. (Ed.). The Cyst Nematodes. Chapman and Hall, London
  • Cook R & Noel G R (2002). Cyst nematodes: Globodera and Heterodera Species, In: Plant Resistance to Parasitic Nematodes (Eds. Star J L, Cook R & Bridge J.), CAB International, pp. 71 – 105
  • İmren M, Toktay H, Özarslandan A, Nicol J M & Elekcioğlu I H (2012). Güneydoğu Anadolu Bölgesi tahıl alanlarında Tahıl kist nematodu, Heterodera avenae group türlerinin belirlenmesi. Türkiye Entomoloji Dergisi 36(2): 265-275
  • İmren M, Toktay H, Bozbuğa R, Erginbaş Orakçı G, Dababat A & Elekcioğlu İ H (2013). Identification of genetic resistance to cereal cyst nematodes; Heterodera avenae (Wollenweber, 1924), H. filipjevi (Madzhidov, 1981) Stelter and H. latipons (Franklin, 1969) in some international bread wheat germplasms. Turkish Journal of Entomology 37(3): 277-282
  • Kort J (1972). Nematode diseases of cereals of temperate climates, In: Webster, J. M. (Ed.). Economic Nematology. Academic Press, New York, 97–126
  • Majnik J, Ogbonnaya F C, Moullet O & Lagudah E S (2003). The Cre1 and Cre3 Nematode Resistance Genes are Located at Homeologous Loci in the Wheat Genome. The American Phytapathological Society. MPMI 16(12): 1135-1144
  • Nicol J M, Rivoal R, Bolat N, Aktas H, Braun H J, Mergoum M, Yıldırım A F, Bağcı A, Elekcioğlu I H & Yahyaoui A (2002a) The frequency and diversity of the cyst and lesion nematodes on wheat in the Turkish Central Anatolian Plateau. Nematology 4(2): 272
  • Nicol, J M (2002b). Important nematode pests. In: Curtis, B. C., Rajaram, S., Gomez Macpherson. H. (Eds.). Bread Whead Improvement and Production. Food and Agriculture Organization of the United Nations, Rome, 345–366
  • Nicol J. M (2009). Occurrence of Cereal Cyst Nematode, Heterodera avenae, in Southeast Anatolia, Turkey, Proceedings of the First Workshop of the International Cereal Cyst Nematode Initiative, Antalya, Turkey, pp. 10-11
  • Özarslandan M, Özarslandan A, Nicol J M & Elekcioğlu İ H (2010). Tahıl kist nematodu, Heterodera filipjevi (Madzhidov, 1981) Stelter’nin patotipinin belirlenmesi ve buğday genotiplerinin, H. filipjevi populasyonlarına karşı dayanıklılıklarının araştırılması. Türkiye Entomoloji dergisi 34(4): 515527
  • Rivoal R & Cook R (1993). Nematode Pests of Cereals, Plant Parasitic Nematodes in Temperate Agriculture,ed: Evans K, Trudgill D L, Webster J M, CAB International: Wallingford, United Kingdom, p. 259-303
  • Rivoal R, Bekal S, Valette S, Gauthier J P, Bel Hadj Fradj M, Mokabli A, Jahier J, Nicol J M & Yahyaoui A (2001). Variation in reproductive capacity and virulence on different genotypes and resistance genes of Triticeae, in the cereal cyst nematode species complex. Nematology 3(6): 581 – 592
  • Rumpenhorst H J, Elekcioğlu I H, Sturhan D, Öztürk G and Enneli S (1996). The Cereal Cyst Nematode Heterodera filipjevi (Madzhidov) in Turkey. Nematologia Mediterranea 24: 135- 138
  • Sasser J N A (1987). Perspective on Nematode Problems Worldwide, Ed: Saxena, M.C., Sikora, R.A., Sarivastava, J.P., Nematode Parasitic to Cereals and Legumes in Temperate Semi-Arid Regions Proceedings of a Workshop Held at Larnaca, Cyprus, pp. 1–12
  • Schmidt A L, Mcintyre C L, Thompson J, Seymour N P & Liu C J (2005). Quantitative trait loci for root lesion nematode (Pratylenchus thornei) resistance in Middle-Eastern landraces and their potential for introgression into Australian bread wheat. Australian Journal of Agricultural Research 56: 1059–1068
  • Scholz U & Sikora R (2004). Hatching behaviour and life cycle of Heterodera latipons Franklin under Syrian
  • Agro – Ecological Conditions. Nematology 6(2): 245 – 256 Shepherd A M (1986). Extraction and Estimation of Cyst Nematodes, ed: Southey J.F., Laboratory Methods for Work with Plant and Soil Nematodes, London, Her Majesty’s Stationary Office, pp: 51-58
  • Slootmaker L, Lange G, Jochemsen G & Schepers J (1974). Monosomic Analysis in Bread Wheat of Resistance to Cereal Root Eelworm. Euphytica 23: 497–503
  • Subbotin S A, Sturhan D, Rumpenhorst H J & Moens M (2003). Molecular and morphological characterisation of the Heterodera avenae species complex (Tylenchida: Heteroderidae). Nematology 5: 515-538 Subbotin S A, Mundo-Ocampo M & Baldwin J G (2010). Systematics of Cyst Nematodes (Nematode: Heteroderinae) Nematology monographs and Perspectives 8A. In: Biology and Evolution. Brill Leiden–Boston, pp.68
  • Şahin E (2010). Orta Anadolu Buğday Alanlarında Önemli Bitki Paraziti Nematodların Belirlenmesi ve Tahıl Kist Nematoda Heterodera filipjevi`nin Biyolojisi ile Mücadelesi Üzerine Çalışmalar. Çukurova Üniversitesi Fen Bilimleri Enstitüsü, Doktora tezi (Basılmamış), Adana
  • Toktay H, Yavuzaslanoğlu E, İmren M, Nicol J M, Elekcioğlu I H & Dababat A (2012). Screening for resistance to Heterodera filipjevi and Pratylenchus thornei in sister lines of spring wheat. Turkish Journal of Entomology 36(4):455-461
  • Tuik (2012). Türkiye İstatistik Kurumu, Bitkisel Üretim İstatistikleri (www.tuik.gov.tr) (Erişim tarihi: Kasım, 2013)

Dayanıklılık Geni Cre1’in Akdeniz Tahıl kist nematodu, Heterodera latipons’a Franklin Karşı Etkinliğinin Araştırılması

Year 2014, Volume: 20 Issue: 3, 261 - 268, 14.08.2014

Mustafa İmren , Ece Börteçine Kasapoğlu Abdelfattah Dababat Halil Toktay İbrahim Halil Elekcioğlu

https://doi.org/10.15832/tbd.26896

Abstract

Tahıl kist nematodları (Heterodera avenae group) buğdayın önemli zararlılarından biri olup, Türkiye’de Heterodera
avenae Wollenweber, H. latipons Franklin ve H. filipjevi (Madzhidov) Stone olmak üzere üç önemli türü farklı buğday
üretim alanlarında saptanmıştır. Ülkemizin makarnalık buğdayın merkezi olan Güneydoğu Anadolu Bölgesi’nde
Akdeniz tahıl kist nematodu, Heterodera latipons’un yaygın olarak bulunduğu, bölge genelinde birçok lokasyonda
ekonomik zarar eşiğinin üstünde popülasyona sahip olduğu rapor edilmiştir. Buğday yetiştiriciliğinde kist nematodları
ile en etkin mücadele yöntemi dayanıklı/tolerans çeşit kullanımı olarak bilinmektedir. Günümüzde buğdayın yabani
türlerinden kültür formlarına aktarılmış dokuz farklı dayanıklılık geni (Cre genleri) mevcuttur. Bu çalışmada dayanıklılık
geni Cre1’in H. latipons’un Gaziantep (Karkamış) popülasyonuna karşı etkinliği araştırılmıştır. Çalışmada genomunda
dayanıklılık geni Cre1 bulunan SILVERSTAR buğday çeşidinin yanı sıra SOKOLL, CALINGIRI, GOLDMARKER,
FRAME, STYLET, PASTOR ve CROC_1/AE.SQUARROSA(224)//OPATA çeşitlerine ait ebeveynlerin melezlenmesi
ile geliştirilen 38 adeti dayanıklılık geni taşıyan, 26 adeti dayanıklılık geni taşımayan toplam 64 adet buğday genotipi
H. latipons’a karşı denemeye alınmıştır. Çalışma sonucunda 64 adet genotipten 20’si nematoda dayanıklı, 32’si orta
dayanıklı ve 12’sinin ise orta hassas olduğu tespit edilmiştir. Genomunda Cre1 geni taşıyan 38 melezden ise 10’nun H.
latipons’a karşı dayanıklı, 20’sinin orta dayanıklı ve 8’nin ise orta hassas olduğu saptanmıştır. Bu çalışma ile dayanıklılık
geni Cre1’in H. latipons’un Gaziantep (Karkamış) popülasyonuna karşı mutlak bir dayanıklılığa sahip olmamakla
birlikte %78 etkinlik oranı ile başarılı olduğu ve ulusal buğday ıslah programlarında H. latipons’a karşı kullanılabileceği
sonucuna varılmıştır. Ayrıca, PASTOR, SOKOLL ve CROC_1/AE.SQUARROSA(224)//OPATA buğday genotiplerinin
H. latipons’a karşı iyi bir dayanıklılık kaynağına sahip olduğu saptanmıştır

Keywords

Tahıl kist nematodu Heterodera latipons Buğday Dayanıklılık

References

  • Agrios G N (1997). Plant Pathology, fourth edition. Academic Press Inc., London
  • Akar T, Calıskan M, Nicol J M, Uranbey S, Sahin E, Yazar S, William M & Braun H J (2009). Molecular characterization of Cereal Cyst Nematode diagnostic markers Cre1 and Cre3 in some winter wheat germplasm and their potential use against Heterodera filipjevi, Field Crops Research 114(2): 320-323
  • Barloy D, Lemoine J, Abelard P, Tanguy A M, Rivoal R & Jahier J (2007). Marker-assisted Pyramiding of Two Cereal Cyst Nematode Resistance Genes From Aegilops variabilis in Wheat. Molecular Breeding 20: 31–40
  • Bekal S, Jahier J & Rivoal R (1998). Host response of different Triticeae to species of the cereal cyst nematode complex in relation to breeding resistant durum wheat. Fundamental Applied Nematology 21: 359-370
  • Boerma H R & Hussey R S (1992). Breeding Plants for Resistance to Nematodes. Journal of Nematology 24(2): 242–252
  • Cook R & Rivoal R (1998). Genetics of resistance and parasitism. In: Sharma, S. B. (Ed.). The Cyst Nematodes. Chapman and Hall, London
  • Cook R & Noel G R (2002). Cyst nematodes: Globodera and Heterodera Species, In: Plant Resistance to Parasitic Nematodes (Eds. Star J L, Cook R & Bridge J.), CAB International, pp. 71 – 105
  • İmren M, Toktay H, Özarslandan A, Nicol J M & Elekcioğlu I H (2012). Güneydoğu Anadolu Bölgesi tahıl alanlarında Tahıl kist nematodu, Heterodera avenae group türlerinin belirlenmesi. Türkiye Entomoloji Dergisi 36(2): 265-275
  • İmren M, Toktay H, Bozbuğa R, Erginbaş Orakçı G, Dababat A & Elekcioğlu İ H (2013). Identification of genetic resistance to cereal cyst nematodes; Heterodera avenae (Wollenweber, 1924), H. filipjevi (Madzhidov, 1981) Stelter and H. latipons (Franklin, 1969) in some international bread wheat germplasms. Turkish Journal of Entomology 37(3): 277-282
  • Kort J (1972). Nematode diseases of cereals of temperate climates, In: Webster, J. M. (Ed.). Economic Nematology. Academic Press, New York, 97–126
  • Majnik J, Ogbonnaya F C, Moullet O & Lagudah E S (2003). The Cre1 and Cre3 Nematode Resistance Genes are Located at Homeologous Loci in the Wheat Genome. The American Phytapathological Society. MPMI 16(12): 1135-1144
  • Nicol J M, Rivoal R, Bolat N, Aktas H, Braun H J, Mergoum M, Yıldırım A F, Bağcı A, Elekcioğlu I H & Yahyaoui A (2002a) The frequency and diversity of the cyst and lesion nematodes on wheat in the Turkish Central Anatolian Plateau. Nematology 4(2): 272
  • Nicol, J M (2002b). Important nematode pests. In: Curtis, B. C., Rajaram, S., Gomez Macpherson. H. (Eds.). Bread Whead Improvement and Production. Food and Agriculture Organization of the United Nations, Rome, 345–366
  • Nicol J. M (2009). Occurrence of Cereal Cyst Nematode, Heterodera avenae, in Southeast Anatolia, Turkey, Proceedings of the First Workshop of the International Cereal Cyst Nematode Initiative, Antalya, Turkey, pp. 10-11
  • Özarslandan M, Özarslandan A, Nicol J M & Elekcioğlu İ H (2010). Tahıl kist nematodu, Heterodera filipjevi (Madzhidov, 1981) Stelter’nin patotipinin belirlenmesi ve buğday genotiplerinin, H. filipjevi populasyonlarına karşı dayanıklılıklarının araştırılması. Türkiye Entomoloji dergisi 34(4): 515527
  • Rivoal R & Cook R (1993). Nematode Pests of Cereals, Plant Parasitic Nematodes in Temperate Agriculture,ed: Evans K, Trudgill D L, Webster J M, CAB International: Wallingford, United Kingdom, p. 259-303
  • Rivoal R, Bekal S, Valette S, Gauthier J P, Bel Hadj Fradj M, Mokabli A, Jahier J, Nicol J M & Yahyaoui A (2001). Variation in reproductive capacity and virulence on different genotypes and resistance genes of Triticeae, in the cereal cyst nematode species complex. Nematology 3(6): 581 – 592
  • Rumpenhorst H J, Elekcioğlu I H, Sturhan D, Öztürk G and Enneli S (1996). The Cereal Cyst Nematode Heterodera filipjevi (Madzhidov) in Turkey. Nematologia Mediterranea 24: 135- 138
  • Sasser J N A (1987). Perspective on Nematode Problems Worldwide, Ed: Saxena, M.C., Sikora, R.A., Sarivastava, J.P., Nematode Parasitic to Cereals and Legumes in Temperate Semi-Arid Regions Proceedings of a Workshop Held at Larnaca, Cyprus, pp. 1–12
  • Schmidt A L, Mcintyre C L, Thompson J, Seymour N P & Liu C J (2005). Quantitative trait loci for root lesion nematode (Pratylenchus thornei) resistance in Middle-Eastern landraces and their potential for introgression into Australian bread wheat. Australian Journal of Agricultural Research 56: 1059–1068
  • Scholz U & Sikora R (2004). Hatching behaviour and life cycle of Heterodera latipons Franklin under Syrian
  • Agro – Ecological Conditions. Nematology 6(2): 245 – 256 Shepherd A M (1986). Extraction and Estimation of Cyst Nematodes, ed: Southey J.F., Laboratory Methods for Work with Plant and Soil Nematodes, London, Her Majesty’s Stationary Office, pp: 51-58
  • Slootmaker L, Lange G, Jochemsen G & Schepers J (1974). Monosomic Analysis in Bread Wheat of Resistance to Cereal Root Eelworm. Euphytica 23: 497–503
  • Subbotin S A, Sturhan D, Rumpenhorst H J & Moens M (2003). Molecular and morphological characterisation of the Heterodera avenae species complex (Tylenchida: Heteroderidae). Nematology 5: 515-538 Subbotin S A, Mundo-Ocampo M & Baldwin J G (2010). Systematics of Cyst Nematodes (Nematode: Heteroderinae) Nematology monographs and Perspectives 8A. In: Biology and Evolution. Brill Leiden–Boston, pp.68
  • Şahin E (2010). Orta Anadolu Buğday Alanlarında Önemli Bitki Paraziti Nematodların Belirlenmesi ve Tahıl Kist Nematoda Heterodera filipjevi`nin Biyolojisi ile Mücadelesi Üzerine Çalışmalar. Çukurova Üniversitesi Fen Bilimleri Enstitüsü, Doktora tezi (Basılmamış), Adana
  • Toktay H, Yavuzaslanoğlu E, İmren M, Nicol J M, Elekcioğlu I H & Dababat A (2012). Screening for resistance to Heterodera filipjevi and Pratylenchus thornei in sister lines of spring wheat. Turkish Journal of Entomology 36(4):455-461
  • Tuik (2012). Türkiye İstatistik Kurumu, Bitkisel Üretim İstatistikleri (www.tuik.gov.tr) (Erişim tarihi: Kasım, 2013)
There are 27 citations in total.

Details

Primary Language Turkish
Journal Section Makaleler
Authors

Mustafa İmren

Ece Börteçine Kasapoğlu This is me

Abdelfattah Dababat This is me

Halil Toktay This is me

İbrahim Halil Elekcioğlu This is me

Publication Date August 14, 2014
Submission Date December 2, 2013
Published in Issue Year 2014 Volume: 20 Issue: 3

Cite

APA İmren, M., Kasapoğlu, E. B., Dababat, A., Toktay, H., et al. (2014). Dayanıklılık Geni Cre1’in Akdeniz Tahıl kist nematodu, Heterodera latipons’a Franklin Karşı Etkinliğinin Araştırılması. Journal of Agricultural Sciences, 20(3), 261-268. https://doi.org/10.15832/tbd.26896

Journal of Agricultural Sciences is published open access journal. All articles are published under the terms of the Creative Commons Attribution License (CC BY).