Abstract
Genetic purity is one of the most important quality criteria required for successful hybrid seed production. In this study, molecular markers were used for assessing the genetic purity and diversity of three commercially important maize F1hybrids (Pasha, Frida and PG1661) and their parental inbred lines. Fifty Simple Sequence Repeats (SSRs) markers were used to analyze samples, also the efficiency of the markers were compared. Twenty three primer pairs among the fifty markers were able to detect polymorphism between the different types of hybrids with an average of 0.69 polymorphism information content (PIC) value. Genetic purity analyses revealed more than 98% homogeneity in the hybrid seeds. The hybrids were grouped into three main clusters. It can be concluded that, molecular markers are efficient to study the genetic purity and diversity in maize hybrids and microsatellites are more accurate marker-type because of their co-dominancy
References
- Ajmone-Marsan P, Castiglioni P, Fusari F, Kuiper M & Motto M (1998). Genetic diversity and its relationship to hybrid performance in maize as revealed by RFLP and AFLP markers. Theoretical and Applied Genetics 96: 219-227
- Anderson J A, Churchill G A & Autrique J E (1993). Optimizing parental selection for genetic linkage maps. Genome 36: 181-186
- Bornet B & Branchard M (2001). Nonanchored inter simple sequence repeat (ISSR) markers: Reproducible and specific tools for genome fingerprinting. Plant Molecular Biology Reporter 19: 209-215
- Dangle G A, Mendum M L, Prins B H, Walker M A, Meredith C P & Simon C J (2001). Simple sequence repeat analysis of a clonally propagated species: a tool for managing a grape germplasm collection. Genomet 44: 432-438
- Daniel I O, Adetumbi J A, Oyelakin O O, Olakojo S A, Ajala M O & Onagbesan S O (2012). Application of SSR markers for genetic purity analysis of parental inbred lines and some commercial hybrid maize (Zea mays L.). American Journal of Experimental Agriculture 2(4): 597-606
- Doyle J J & Doyle J L (1990). Isolation of plant DNA from fresh tissue. Focus 12: 13-15
- Dubreuil P & Charcosset A (1998). Genetic diversity within and among maize populations: A comparison between isozyme and nuclear RFLP loci. Theoretical and Applied Genetics 96: 577-587
- FAO (2012). Statistical database. Available at: http:// www.fao.org
- Gül İnce A, Karaca M & Onus A N (2011). Exact microsatellite density differences among capsicum tissues and development stages. Tarım Bilimleri
Abstract
Genetik saflık, hibrit tohum üretimi için gerekli olan önemli kalite kriterlerinden biridir. Bu çalışmada, moleküler işaretleyiciler ticari olarak önemli 3 mısır F1 hibrit çeşidi (Pasha, Frida ve PG1661) ve kendilenmiş saf ebeveyn hatlarının genetik saflık ve çeşitlilik analizleri için kullanılmıştır. Örnekler, elli adet basit dizi tekrarları (SSR) işaretleyicileri kullanılarak analiz edilmiş ve işaretleyicilerin verimliliği karşılaştırılmıştır. Testlenen 50 işaretleyici içinden 23 primer çifti ortalama 0.69 polimorfizm bilgisi değeri ile değişik hibrit çeşitleri arasındaki farklılığı tespit etmiştir. Hibrit tohumların genetik saflık analizi, % 98’den yüksek homoloji oranı ile sonuçlanmıştır. Hibritler, 3 ana gruba toplamıştır. Bu çalışma ile moleküler işaretleyicilerin mısır hibritlerinin genetik saflık ve çeşitlilik analizleri için kullanılmasının verimli olduğu ve mikrosatellitlerin ko-dominantlık özellikleri nedeni ile doğru işaretleyiciler olduğu saptanmıştır.
References
- Ajmone-Marsan P, Castiglioni P, Fusari F, Kuiper M & Motto M (1998). Genetic diversity and its relationship to hybrid performance in maize as revealed by RFLP and AFLP markers. Theoretical and Applied Genetics 96: 219-227
- Anderson J A, Churchill G A & Autrique J E (1993). Optimizing parental selection for genetic linkage maps. Genome 36: 181-186
- Bornet B & Branchard M (2001). Nonanchored inter simple sequence repeat (ISSR) markers: Reproducible and specific tools for genome fingerprinting. Plant Molecular Biology Reporter 19: 209-215
- Dangle G A, Mendum M L, Prins B H, Walker M A, Meredith C P & Simon C J (2001). Simple sequence repeat analysis of a clonally propagated species: a tool for managing a grape germplasm collection. Genomet 44: 432-438
- Daniel I O, Adetumbi J A, Oyelakin O O, Olakojo S A, Ajala M O & Onagbesan S O (2012). Application of SSR markers for genetic purity analysis of parental inbred lines and some commercial hybrid maize (Zea mays L.). American Journal of Experimental Agriculture 2(4): 597-606
- Doyle J J & Doyle J L (1990). Isolation of plant DNA from fresh tissue. Focus 12: 13-15
- Dubreuil P & Charcosset A (1998). Genetic diversity within and among maize populations: A comparison between isozyme and nuclear RFLP loci. Theoretical and Applied Genetics 96: 577-587
- FAO (2012). Statistical database. Available at: http:// www.fao.org
- Gül İnce A, Karaca M & Onus A N (2011). Exact microsatellite density differences among capsicum tissues and development stages. Tarım Bilimleri
Details
| Primary Language | English |
|---|---|
| Journal Section | Makaleler |
| Authors | |
| Publication Date | March 11, 2015 |
| Submission Date | March 12, 2014 |
| Published in Issue | Year 2015 Volume: 21 Issue: 2 |
Cited By
Genetic Diversity of Maize Accessions (<i>Zea mays</i> L.) Cultivated from Benin Using Microsatellites Markers
American Journal of Molecular Biology
Hafiz A. Salami
https://doi.org/10.4236/ajmb.2016.61002
Journal of Agricultural Sciences is published open access journal. All articles are published under the terms of the Creative Commons Attribution License (CC BY).