The effects of <em>Azadirachta indica </em>A. Juss. extracts on development on larvae of Colorado Potato Beetle [<em>Leptinotarsa decemlineata</em> Say (Col.:Chrysomelidae)]

Year 2010, Volume: 50 Issue: 2, 73 - 88, 19.07.2010

Pervin Erdoğan , Seval Toros

Abstract

The effects of Neem Azal T/S and Neemix preparations obtained from Azadirachta indica A. Juss. were investigated on development larvae of Leptinotarsa decemlineata Say.

A. indica preparations were tested to be alternative to chemical insecticide to control L. decemlineata. Bioassays to determine the effect of varying concentrations of extracts were used in three different methods. Third instar larvae of about 30-35 mg weight were used for leaf dipping, larvae dipping and topical application. For all experiments 2.2 cm in diameter leaf disks obtained from potatoes grown in the glasshouse were used. For three bioassays four different concentrations were used, for Neem Azal T/S 15, 25, 50, 100 ppm and Neemix 10, 15, 30, 60 ppm. All of experiments were repeated six times.

The results were determined at larvae stage that duration of larvae and pupae periods have been prolonged for all the methods, high mortality at larvae and pupae stages have been obtained, malformed individuals came out, number of individuals from pupae were decreased and the number of eggs laid by the healthy females of the treated pupae decreased.

Keywords

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<em>Azadirachta indica</em> A. Juss ekstraktlarının Patates böceği [<em>Leptinotarsa decemlineata</em> Say (Col.: Chrysomelidae)]

Abstract

p>Azadirachta indica A. Jussbitkisindenelde edilmiş Neem Azal T/S ve Neemix preparatlarının Patates böceği (Leptinotarsa decemlineata Say) larvalarının gelişimine etkileri araştırılmıştır.

A indica preparatlarının etkisini belirlemek için üç farklı yöntem kullanılmıştır. Yaprak daldırma, bireyi daldırma ve topikal aplikasyon yöntemleri 30-35 mg ağırlığındaki üçüncü dönem larvalara uygulanmıştır. Bütün denemelerde 2.2cm çapında serada yetiştirilen patates yapraklarından kesilen diskler kullanılmıştır. Her üç yöntem için, Neem Azal T/S,15, 25, 50, 100 ppm dozlarında ve Neemix 10, 15, 30 ve 60 ppm dozlarında olmak üzere dört farklı dozda kullanılmıştır. Bütün denemeler 6 tekerrürlü olarak yapılmıştır.

Araştırma sonucunda, larva döneminde uygulanan tüm yöntemlerde preparatların doz artışına bağlı olarak, larva ve pupa dönemi süresini uzattığı, larva ve pupa dönemlerinde yüksek oranda ölüme neden olduğu, anormal görünümlü bireylerin meydana geldiği, pupadan çıkan ergin sayısının azaldığı ve pupadan çıkan sağlıklı dişilerin daha az yumurta bıraktığı belirlenmiştir.

Keywords

Leptinotarsa decemlineata; Larva; Pupa; Gelişime etki; Fekundite; Neem Azal T/S; Neemix; larvae; pupa; growth effect; fecundity; Neem Azal T/S; Neemix

References

• Anonim 2006.Tarımsal Yapı ve Üretim, Başbakanlık DİE No: Ankara
• Anonim 2009. http://www.epa.gov. (Erişim tarihi: 20.10.2009)
• Ascher K.R.S. Eliyahum M. Nemny N.E. and Meisner J. 1983. Neem seed kernel extracts as an inhibitor of growth and fecundity in Spodoptera littoralis. Proc. 2nd. Int. Neem Conf.(Raiuishholzhausen, 1983),pp 331-344.
• Atak U. 1973. Trakya Bölgesinde Patates bceği (Leptinotarsa decemlineata (Say)‘nin morfolojisi, bio-ekolojisi ve savaş metotları üzerinde araştırmalar. T.C Tarım Bakanlığı Zirai Mücadele ve Zirai Karantina Genel Müdürlüğü Yayınları, Teknik Bülten 6, 63 s.
• Barnaby M.A. Yamasaki R.B. and Klocke J.A. 1989. Biological activty of azadirachtin,three derivatives, and their ultraviolet radiation degration products against tobacco budworm (Lepidoptera: Noctuidae) larvae. J.Econ. Entomol. 82(1):58-63.
• Baumgart M. Brocke K. and Crow M.H. 1996. Control of the Colorado potato beetle (Leptinotarsa decemlineata (Say) in organic Gardening Systems with Bacillus thuringiensis var. San. Diego (M-one) and new product from the neem tree (Neem Azal F- and Align) Proceedings at the 5th workshop. Wetzlar, Germany, Jan, 22-25 1996.
• Chopra R. L. 1928. Ann. Rep. of the Entomologist to the Govt.of Punjab, Lyallpur, for the year 1925-26. Rep.Dept. Agric. Puncab. 1(pt.2):67-125.
• Dorn A., Rademacher J.M. and Sehn E. 1986. Effects of azadirachtin on the moulting cycle, endocrine system,and ovaries in last-instar larvae of the milkweed bug, Oncepaltus fasciatus J. Insect Physiol. 32(3), 231-238.
• Erkılıç L.,Yumruktepe R., Ulubilir A. ve Aytaş M. 1986.T.C. Tarım ve Köyişleri Bakanlığı II. Ulusal Zirai Mücadele Simpozyumu. Ankara 156-167.
• Ferizli A.G.1997. Azadirachta indica A. Juss ve Melia azedarach L. (Meliaceae) süzütlerinin Spodoptera littoralis Boisd (Lep: Noctuidae)‘e Bazı Etkileri Üzerinde Araştırmalar (Yayımlanmamış Doktora tezi) .
• Gujar G.T. and Mehrotra K.N.1983. Inhibition oh growth and development of the tobacco caterpiller Spodoptera litura Fabr. Due to azadirachtin and neem products. Indian J.Ent.45(4),431-435.
• Hiiesaar K. Metspalu L., Joüdu J. and Kuusik A. 2004. Diverse effects of Neem Azal T/S revealed by preimaiginal stages of Colorado potato betle, Leptinotarsa decemlineata Say. Proceedings of the 9th Workshop. Hohensolmos, Germany.
• Kaethner M.1992. Fitness reduction and mortality effects of neem-based pesticides on the Colorado potato beetle Leptinotarsa decemlineata Say (Col: Chrysomelidae). J.Appl. Ent.113 (1992), 456-465.
• Ladd T. L. Jr. Warthen J. D. Jr. and Klein M.G. 1984. Japanase Beetle (Coleoptera: Scarabaeidae): The effects of azadirachtin on the growth and development of the immature forms. J. Econ.Entomol. 77,903-905.
• Nicol C.M.Y. and Schmutterer H. 1991. Kontaktwirkungen des samenöls des neimbaumes Azadirachta indica A.Juss bei gregaren larven der wüstenheuschrecke Schistocerca gregaria (Forskal) J. Appl. Ent.111 (1991), 197-205.
• Oroumchi S. and Lorra C.1993. Investigation on the effects of aqueous extracts of neem and chinaberry on development and mortality of alfalfa weevil Hyperia postica Gyllenh.(Col., Curculionidae) J. Appl.Ent. 116 (1993), 345-351.
• Pereira D. and Wohlgemuth R. 1982. Neem (Azadirachta indica A.Juss ) of west african origin as a protectant of stored maize. J. Appl. Ent. 94: 208-214.
• Prijiono D. and E. Hassan.1993. Laboratory and field efficacy of neem (Azadirachta indica A.Juss) extracts agains two broccoli pest. Zeitschrift für Pflanzenkrankenheiten und Pflanzenschutz. Journal of Plant Dieases and Protection 100 (4), 354-370.
• Rao P.J.and Subrahmanyam B.1986. Azadirachtin induced changes in development, food utilazitation and haemolymph constituents of Schistocerca gregaria Forskal. J.Appl.Ent.102,217-224.
• Rembold H. Sharma G.K. Czoppelt CH. and Schmutterer H. 1982. Azadirachtin A patent insect growth regulator of plant origin. J. Appl. Ent. 93, 12-17.
• Stark J.D., Vargas R.I., and Thalman R.K. 1990. Azadirachtin effects on metamorphosis longevity and reproduction of three tephritid fruit fly species (Diptera: Tephritidae). J. Econ. Entomol. 83(6): 2168-2174.
• Steets R. 1976. Studies on the effect of a purified extract of fruits of Azadirachta indica on Leptinotarsa decemlineata Say (Col: Chrysomelidae).Z.ang. Ent. 82, 169-176.
• Schlüter U. Bidmon H. J. and Grewe S. 1985. Azadirachtin effects growth and endocrine events in larvae of the tobacco hornworm, Menduca sexta. J. Insect
• Schmutterer H. 1981.Some properties of components of the neem tree (Azadirachta indica) and their use in pest control in developing countries. Med. Fac. Landbouww. Rijksuniv. Gent, 46/1, 39-47.
• Schmutterer H. 1990. Properties and Potential of Natural Pesticides from the neem tree , Azadirachta indica. Annu.Rev.Entomol.1990. 35:271-97.
• Steets R. and Schmutterer H. 1975. The effect of azadirachtin on the longevity and reproduction of Epilachna varivestis Mulls. (Coleoptera: Coccinellidae). Z.pfl krank. 3/75 176-179.
• Varnagy L. 2006. Feeding inhibitors effects of several extracts of plant and animal origin on some phytophagus insect species. D. Sc. Universitiy of Veszprem Georgikon Faculty of Agriculture Interdiscplinary doctoral School. Head of School.
• Verkerk R.H.J. and Wright D.J. 1993. Biological activity of neem seed kernel extracts and synthetic azadirachtin against larvae of Plutella xylostella L. Pestic. Sci. 1993, 37, 83-91.
• Wardajo S. 1969. Some farctors relating to the larval growth of the Colorado potato beetle, Leptinotarsa decemlineata Say (Col.: Chrysomelidae), on articial diets. Mededelingen Landbuowhogeschool Wagenigeningen, Nederland, Mededeling No. 166,71 s.
• Zebitz C.P.W. 1984. Effect of some crude and azadirachtin- enriched neem (Azadirachta indica) seed kernel extracts on larvae of Aedes aegypti. Entomal. exp.appl. 35, 22- 16.
• Zehnder G. and Warthen J.D. 1988. Feeding inhibition and mortality effect of neem-seed extract on the Colorado potato beatle (Coleoptera: Chrysomelidae). J.Econ. Entomol. 81(4): 1040-1044.