Beauveria bassiana (Balsamo) Vuillemin'in Patates böceği (Leptinotarsa decemlineata Say.) (Coleoptera: Chrysomelidae)'ne karşı etkisinin araştırılması

Year 2024, Volume: 64 Issue: 3, 37 - 44, 01.10.2024

Dr. Semiha Yüceer

https://doi.org/10.16955/bitkorb.1426255

Abstract

Entomopatojen fungusların Patates böceği (Leptinotarsa decemlineata) üzerindeki etkinliğini belirlemek amacıyla bu çalışma yapılmıştır. Bu amaçla patates ekim alanlarında sürveyler yapılarak entomopatojen fungusların izolasyonu için ölü patates böcekleri ile toprak örnekleri toplanmıştır. Yapılan analizler sonucunda elde edilen 30 adet entomopatojen fungus ile patajonisite testleri gerçekleştirilmiştir. Biyolojik etkinlik sonuçlarına göre L. decemlineata’ya karşı %75 etki oranında EP-1 izolatı belirlenmiş ve tanısı yapılmıştır. Beauveria bassiana’nın (EP-1) en etkili izolat olduğu belirlenmiştir. Doz belirleme çalışmalarında ise, B. bassiana izolatından 106, 107 ve 108 konidi ml/l dozları kullanılmıştır. Yapılan çalışmalarda, her doz ve kontrol grubu için beş tekrarlı deneme kurulmuştur. Kontrollü koşullarda saksılarda yetiştirilen patates bitkileri üzerindeki ergin, 2. ve 3. dönem Patates böceği larvalarına entomopatojen fungusların spor süspansiyonu püskürtülmüştür. Canlı bireylerin sayısı, uygulamadan sonraki 1., 3., 5., 7. ve 9. günlerde kaydedilmiş ve ölüm yüzdesini hesaplamak için kullanılmış, B. bassiana izolatının en etkili dozu, 108 konidi ml/l dozuyla uygulamanın 7. gününde %85 ölüm olarak belirlenmiştir. Elde edilen verilere göre, ölüm oranlarının dozun artmasıyla 1., 3., 5., 7. ve 9. günlerde arttığı gözlemlenmiştir. En yüksek etki, 108 konidi ml/l dozuyla yapılan uygulamalarda gözlenmiştir. Sonuç olarak, insanlar ve çevre için daha az zararlı olan entomopatojen fungus izolatı B. bassiana’nın, L. decemlineata'ya karşı biyolojik kontrol ajanı olarak kullanılması uygun görülmektedir.

Keywords

patates, patates böceği, biyolojik kontrol, ölüm oranı

Investigation of the effect of Beauveria bassiana (Balsamo) Vuillemin against potato beetle (Leptinotarsa decemlineata Say.) (Coleoptera: Chrysomelidae)

Abstract

In this study, the objective was to determine the efficacy of entomopathogenic fungi on the Colorado potato beetle [Leptinotarsa decemlineata Say. (Coleoptera: Chrysomelidae)]. To reach this goal, surveys were conducted in potato fields, and dead L. decemlineata and soil samples were collected to isolate entomopathogenic fungi. Pathogenicity tests were carried out using 30 entomopathogenic fungi that were obtained as a result of the analyses. According to the biological activity results, EP-1 isolate was identified and diagnosed as having 75% efficacy against L. decemlineata. Beauveria bassiana (EP-1), was identified as the most effective isolate. In the dose determination studies, 106, 107, and 108 conidia ml/l doses of B. bassiana's most effective isolate were used. The experiments were set up with five replications for each dose and control group. The spore suspension of the entomopathogenic fungus was sprayed on fully-grown potato plants cultivated in pots, targeting mature and 2nd or 3rd instar L. decemlineata. The number of live individuals was recorded on the 1st, 3rd, 5th, 7th, and 9th days after application to calculate the percentage of mortality. The most effective dose of B. bassiana isolate was determined to be 85% mortality on the 7th day after application with a dose of 108 conidia ml/l. According to the obtained data, it was observed that the mortality rates increased with the increase in dose on the 1st, 3rd, 5th, 7th, and 9th days after application. The highest impact was observed in applications with a dose of 108 conidia ml/l. In conclusion, the entomopathogenic fungus B. bassiana isolate, which is less harmful to humans and the environment, is considered suitable for use as a biological control agent against L. decemlineata.

Keywords

potato, potato beetle, biological control, mortality

Supporting Institution

Biological Control Research Institute

Thanks

The study was supported by the Ministry of Agriculture and Forestry General Directorate of Agricultural Research and Policies.

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