Effect of tadalafil treatment on ovarian ischemia injury in rats

Year 2021, Volume: 46 Issue: 1, 55 - 62, 31.03.2021

Dilan Altıntaş Ural , Duygun Altıntaş Aykan , Sezen Koçarslan , Adem Doğaner

Abstract

Purpose: Ovarian ischemia, which is known as one of the most common gynecologic surgery emergencies in women of all ages, is the distruption of the ovarian perfusion. Herein, we aimed to investigate the effect of tadalafil, a phosphodiesterase-5 inhibitor, at different doses and durations in the management of ovarian survival in ischemia-reperfusion injury model in rats. Materials and Materials and Methods: Thirty female Wistar albino rats were divided into five groups. Ovarian ischemia was induced by clamping the adnex of uterine tuba for three hours and reperfused for one hour. Drug treatments were given as follows: Group 1: saline (ischemic control), Group 2: tadalafil 1 mg/kg, Group 3: tadalafil 10 mg/kg, Group 4: tadalafil 1 mg/kg for 7 days, Group 5: tadalafil 10 mg/kg for 7 days. At the end of the treatments, ovaries were removed and evaluated for histopathological analysis. Results: Group 4 and Group 5 showed significant decrease in vascular congesion, hemorrhage, edema, neutrophilic infiltration compared to Group 1. All groups showed significant decrease in neutrophilic infiltration compared to Group 1. We found that treatment with tadalafil for 7 days significantly decreased the ovarian tissue damage scores in rats compared to the ischemic control.
Conclusion: Our results showed that tadalafil treatment in ischemic ovarian injury is effective in limiting ovarian damage. The reduction in the tissue damage scores suggested that treatment with PDE5 inhibitors, such as tadalafil, may contribute to the healing of conservatively treated ovaries and may play a role in the treatment of adnexal torsion.

Keywords

tadalafil, ovary, ischemia

Tadalafil tedavisinin ratlarda over iskemi hasarına etkisi

Abstract

Amaç: Her yaştan kadında en sık görülen jinekolojik cerrahi acil durumlarından biri olarak bilinen over iskemisi, yumurtalık perfüzyonunun bozulmasıdır. Sıçanlarda iskemi-reperfüzyon hasarı deneysel modelinde bir fosfodiesteraz-5 inhibitörü olan tadalafilin farklı doz ve tedavi süresinde over sağkalımı üzerindeki etkisini araştırmayı amaçladık.
Gereç ve Yöntem: Otuz dişi Wistar albino sıçan beş gruba ayrıldı. Yumurtalık iskemisi, tuba uterina adneksinin üç saat klemplenmesi ve bir saat reperfüze edilmesi suretiyle gerçekleştirildi. İlaç tedavileri şu şekilde verildi: Grup 1: salin (iskemik kontrol), Grup 2: tadalafil 1 mg / kg, Grup 3: tadalafil 10 mg / kg, Grup 4: tadalafil 1 mg / kg x 7 gün, Grup 5: tadalafil 10 mg / kg x 7 gün. Tedavilerin sonunda overleri çıkarıldı ve histopatolojik olarak değerlendirildi.
Bulgular: Grup 4 ve Grup 5'te, Grup 1'e kıyasla vasküler konjesyon, kanama, ödem ve nötrofilik infiltrasyon anlamlı derecede daha azdı. Tüm gruplar, Grup 1'e kıyasla nötrofilik infiltrasyonda anlamlı düşüş gösterdi. 7 günlük tadalafil tedavisinin iskemik kontrol grubu ile karşılaştırıldığında sıçanlardaki over doku hasarını belirgin derecede azalttığını tespit ettik.
Sonuç: Bu çalışmadaki sonuçlar overin iskemik hasarında tadalafil tedavisinin bu hasarını sınırlamada etkili olduğunu göstermiştir. Doku hasarı skorlarındaki azalma, tadalafil gibi PDE-5 inhibitörleri ile tedavinin konservatif olarak tedavi edilen overlerin iyileşmesine katkıda bulunabileceğini ve adneksiyal torsiyonun tedavisinde rol alabileceğini ortaya koymaktadır.

Keywords

Tadalafil, iskemi, over

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