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Kadmiyumla stres oluşturulan Citrobacter freundii’nin vitamin içeriği ve stres belirteçlerine selenyumun etkisi

Year 2021, , 697 - 707, 15.09.2021

Muhammad Salihu Ibrahim , Meltem Çakmak , Dursun Özer , Fikret Karataş , Sinan Saydam

https://doi.org/10.35234/fumbd.947044

Abstract

Bu çalışmada, Citrobacter freundii farklı konsantrasyonlarda kadmiyum içeren LB besiyerinde üretildi. Kadmiyum içeren besi yerine değişik konsantrasyonlarda selenyum katılarak, üretilen hücre konsantrasyonu, ve bazı biyokimyasal parametreler spektrofotometre ve HPLC ile belirlendi. Bakteriler 0 (kontrol), 75, 100, 125 ppm kadmiyum (Cd) içeren besi ortamlarında üretildi. Kontrol ile 75, 100 ve 125 ppm Cd içeren gruplar karşılaştırıldığında, bakteri, vitaminler ve indirgenmiş glutatyonun (GSH) konsantrasyonlarında azalma, yükseltgenmiş glutatyon (GSSG), malondialdehit (MDA) ve 4-hidroksineoneal (4-HNE) konsantrasyonlarında ise artış gözlendi (p<0.05). 75, 100 ve 125 ppm Cd içeren besi ortamına 1.0 ve 3.0 ppm selenyum (Se) katılmasıyla birlikte, bakteri, vitaminler ve GSH konsantrasyonlarında artış, GSSG, MDA ve 4-HNE konsantrasyonlarında ise azalma gözlendi. Bu bulgulardan, kadmiyumun olumsuz etkisini, bakteri besi yerine katılan selenyumun azalttığı söylenebilir

Keywords

Citrobacter freundii kadmiyum selenyum vitaminler

Supporting Institution

Fırat Üniversitesi

Project Number

FUBAP FF.18.15

Thanks

Fırat Üniversitesi, Bilimsel Araştırma Projeleri birimi (FÜBAP) tarafından FF 18.15 numaralı proje ile maddi olarak desteklenmiştir

References

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  • [2] O'Hara CM, Westbrook GL, Miller JM. Evaluation of Vitek GNI+ and Becton Dickinson Microbiology Systems Crystal E/NF identification systems for identification of members of the family Enterobacteriaceae and other gram-negative, glucose-fermenting and non-glucose-fermenting bacilli. J Clin Microbiol 1997; 35: 3269-3273.
  • [3] Puchenkova S. Enterobacteria in areas of water along the Crimean Coast. Mikrobiolohichnyi Zhurnal (Kiev, Ukraine: 1993) 1996; 58: 3-7.
  • [4] Awuchi CG, Igwe VS, Amagwula IO, Echeta CK. Health Benefits of Micronutrients (Vitamins and Minerals) and their Associated Deficiency Diseases: A Systematic Review. Int J Food Sci 2020; 3(1): 1-32
  • [5] Cnubben NHP, Rıetjens IMCM, Wortelboer H, Van Zanden J, Van Bladeren PJ. The interplay of glutathione-related processes in antioxidant defense. Environ Toxicol Pharmacol 2001;10: 141-152.
  • [6] Banfalvi G. Cellular Effects of Heavy Metals. Netherlands, London, New York: Springer; 2011.
  • [7] Beyersmann D, Hartwig A. Carcinogenic metal compounds: recent insight into molecular and cellular mechanisms. Arch Toxikol 2008; 82, 493-512.
  • [8] Burcu Uslu, Şule Aktaç. Selenyum ve Depresyon Üzerine Etkileri. Avrupa Bilim ve Teknoloji Dergisi 2020; 20: 147-151
  • [9] Ibrahim M, Ibrahim Y, Mukhtar Z, Karatas F. Amount of vitamin A, vitamin E, vitamin C, malondialdehyde, glutathione, ghrelin, beta-carotene, lycopene in fruits of Hawthorn, Midland (Crataegus laevigata). J Hum Nutr Food Sci 2017; 5: 1112-1117.
  • [10] Ligor M, Ligor T, Gadzała‐Kopciuch R, Buszewski B. The chromatographic assay of 4‐hydroxynonenal as a biomarker of diseases by means of MEPS and HPLC technique. Biomed Chromatogr 2015; 29: 584-589.
  • [11] Miller KW, Lorr NA, Yang CS. Simultaneous determination of plasma retinol, α-tocopherol, lycopene, α-carotene, and β-carotene by high-performance liquid chromatography. Anal Biochem 1984; 138: 340-345.
  • [12] Amidžić R, Brborić J, Čudina O, Vladimirov S. Rp-HPLC determination of vitamins, folic acid and B12 in multivitamin tablets. J Serbian Chem Society 2005; 70: 1229-1235.
  • [13] Dawes P. Dawes E. SGE Choromatography Products Catalog. (2000). pg: 182.
  • [14] Karatas F, Karatepe M, Baysar A. Determination of free malondialdehyde in human serum by high-performance liquid chromatography. Anal Biochem 2002; 311: 76-79.
  • [15] Yiğit A, Aktaş N, Şahan T. Lactobacillus Brevis Mikroorganizmasının Çoğalma Kinetiğinin Cevap Yüzeyi Yöntemi ile İncelenmesi, YY Üniv Fen Bil Enst Derg/ J Inst Nat Appl Sci 2013; 18(1-2): 25-32
  • [16] Monballiu A, Cardon N, Tri Nguyen M, Cornelly C, Meesschaert B, Chiang YW. Tolerance of Chemoorganotrophic Bioleaching Microorganisms to Heavy Metal and Alkaline Stresses. Bioinorg Chem Appl 2015; 2015(), 1–9. doi:10.1155/2015/861874.
  • [17] Çınar M. Kadmiyumun biyolojik sistemdeki etkileri. Veterinarium 2003; 14(1): 79-84
  • [18] Güner U. Heavy metal effects on P, Ca, Mg, and total protein contents in embryonic pleopodal eggs and stage-1 juveniles of freshwater crayfish Astacus leptodactylus (Eschscholtz, 1823). Turk J Biol 2010; 34: 405-412.
  • [19] Brown KM, Arthur JR. Selenium, selenoproteins and human health:a review. Public Health Nutr 2001; 4(2B): 593–599
  • [20] El-Demerdash FM, Nasr HM. Antioxidant effect of selenium on lipid peroxidation, hyperlipidemia and biochemical parameters in rats exposed to diazinon. J Trace Elem Med Biol 2014; 28(1): 89–93
  • [21] Hoffmann PR, Berry MJ. The influence of selenium on immune responses. Mol Nutr Food Res 2008; 52:1273–1280
  • [22] Araúz ILC, Afton S, Wrobel K, Caruso JA, Corona JFG, Wrobel K. Study on the protective role of selenium against cadmium toxicity in lactic acid bacteria: An advanced application of ICP-MS. J Hazard Mater 2008; 153: 1157-1164
  • [23] Vinceti M, Filippini T, Cilloni S, Bargellini A, Vergoni AV, Tsatsakis A, Ferrante M. Health risk assessment of environmental selenium: Emerging evidence and challenges. Mol Med Rep 2017; 15(5): 3323-3335.
  • [24] Gutteridge JMC, Halliwell B. The Deoxsiriboz Assay Both for Free Radical and for Site Specific Hydroxyl Radical Production. Biochem 1988; 253-932.
  • [25] Ognjanovic BJ, Pavlovic SZ, Maletic D, Zikic RV, Stajn AS, Radojicic RM, Saicic ZS, Petrovic VM. Protective influence of vitamin E on antioxidant defense system inthe blood of rats treated with cadmium. Physiol Res 2003; 52: 563- 570.
  • [26] Bolander FF. Vitamins: not just for enzymes, Current opinion in investigational drugs (London, England: 2000) 2006; 7(10): 912-915
  • [27] Muhamma I, Ashiru S, Kanoma A, Sani I, Garba S. Effect of ripening stage on vitamin C content in selected fruits. Int J Agric Forestry Fisheries 2014; 2: 60-65
  • [28] Kumar A, Pandey R, Siddiqi N. Oxidative stress biomarkers of cadmium toxicity in mammalian systems and their distinct ameliorative strategy. J Appl Biotechnol Bioeng 2019; 6: 126-135.
  • [29] Gaweł S, Wardas M, Niedworok E, Wardas P. Malondialdehyde as lipid peroxidation marker. Wiad Lek 2004; 57(9-10): 453-455.
  • [30] Schaur RJ, Siems W, Bresgen N, Eckl PM. 4-Hydroxy-nonenal—a bioactive lipid peroxidation product. Biomolecules 2015; 5: 2247-2337.
  • [31] Choudhary M, Jetley UK, Khan MA, Zutshi S, Fatma T. Effect of heavy metal stress on proline, malondialdehyde, and superoxide dismutase activity in the cyanobacterium Spirulina platensis-S5. E Ecotoxicol Environ Saf 2007; 66: 204- 209.
  • [32] Corticeiro SC, Lima AIG, Figueira EMdAP. The importance of glutathione in oxidative status of Rhizobium leguminosarum biovar viciae under Cd exposure. Enzyme Microb Technol 2006; 40: 132-137.
  • [33] Zwolak I. The role of selenium in arsenic and cadmium toxicity: an updated review of scientific literature. Biol Trace Elem Res 2020; 193: 44-63.

Year 2021, , 697 - 707, 15.09.2021

Muhammad Salihu Ibrahim , Meltem Çakmak , Dursun Özer , Fikret Karataş , Sinan Saydam

https://doi.org/10.35234/fumbd.947044

Abstract

Project Number

FUBAP FF.18.15

References

  • [1] Demain AL, Vaishnav P. Production of recombinant proteins by microbes and higher organisms. Biotechnol Adv 2009; 27: 297-306.
  • [2] O'Hara CM, Westbrook GL, Miller JM. Evaluation of Vitek GNI+ and Becton Dickinson Microbiology Systems Crystal E/NF identification systems for identification of members of the family Enterobacteriaceae and other gram-negative, glucose-fermenting and non-glucose-fermenting bacilli. J Clin Microbiol 1997; 35: 3269-3273.
  • [3] Puchenkova S. Enterobacteria in areas of water along the Crimean Coast. Mikrobiolohichnyi Zhurnal (Kiev, Ukraine: 1993) 1996; 58: 3-7.
  • [4] Awuchi CG, Igwe VS, Amagwula IO, Echeta CK. Health Benefits of Micronutrients (Vitamins and Minerals) and their Associated Deficiency Diseases: A Systematic Review. Int J Food Sci 2020; 3(1): 1-32
  • [5] Cnubben NHP, Rıetjens IMCM, Wortelboer H, Van Zanden J, Van Bladeren PJ. The interplay of glutathione-related processes in antioxidant defense. Environ Toxicol Pharmacol 2001;10: 141-152.
  • [6] Banfalvi G. Cellular Effects of Heavy Metals. Netherlands, London, New York: Springer; 2011.
  • [7] Beyersmann D, Hartwig A. Carcinogenic metal compounds: recent insight into molecular and cellular mechanisms. Arch Toxikol 2008; 82, 493-512.
  • [8] Burcu Uslu, Şule Aktaç. Selenyum ve Depresyon Üzerine Etkileri. Avrupa Bilim ve Teknoloji Dergisi 2020; 20: 147-151
  • [9] Ibrahim M, Ibrahim Y, Mukhtar Z, Karatas F. Amount of vitamin A, vitamin E, vitamin C, malondialdehyde, glutathione, ghrelin, beta-carotene, lycopene in fruits of Hawthorn, Midland (Crataegus laevigata). J Hum Nutr Food Sci 2017; 5: 1112-1117.
  • [10] Ligor M, Ligor T, Gadzała‐Kopciuch R, Buszewski B. The chromatographic assay of 4‐hydroxynonenal as a biomarker of diseases by means of MEPS and HPLC technique. Biomed Chromatogr 2015; 29: 584-589.
  • [11] Miller KW, Lorr NA, Yang CS. Simultaneous determination of plasma retinol, α-tocopherol, lycopene, α-carotene, and β-carotene by high-performance liquid chromatography. Anal Biochem 1984; 138: 340-345.
  • [12] Amidžić R, Brborić J, Čudina O, Vladimirov S. Rp-HPLC determination of vitamins, folic acid and B12 in multivitamin tablets. J Serbian Chem Society 2005; 70: 1229-1235.
  • [13] Dawes P. Dawes E. SGE Choromatography Products Catalog. (2000). pg: 182.
  • [14] Karatas F, Karatepe M, Baysar A. Determination of free malondialdehyde in human serum by high-performance liquid chromatography. Anal Biochem 2002; 311: 76-79.
  • [15] Yiğit A, Aktaş N, Şahan T. Lactobacillus Brevis Mikroorganizmasının Çoğalma Kinetiğinin Cevap Yüzeyi Yöntemi ile İncelenmesi, YY Üniv Fen Bil Enst Derg/ J Inst Nat Appl Sci 2013; 18(1-2): 25-32
  • [16] Monballiu A, Cardon N, Tri Nguyen M, Cornelly C, Meesschaert B, Chiang YW. Tolerance of Chemoorganotrophic Bioleaching Microorganisms to Heavy Metal and Alkaline Stresses. Bioinorg Chem Appl 2015; 2015(), 1–9. doi:10.1155/2015/861874.
  • [17] Çınar M. Kadmiyumun biyolojik sistemdeki etkileri. Veterinarium 2003; 14(1): 79-84
  • [18] Güner U. Heavy metal effects on P, Ca, Mg, and total protein contents in embryonic pleopodal eggs and stage-1 juveniles of freshwater crayfish Astacus leptodactylus (Eschscholtz, 1823). Turk J Biol 2010; 34: 405-412.
  • [19] Brown KM, Arthur JR. Selenium, selenoproteins and human health:a review. Public Health Nutr 2001; 4(2B): 593–599
  • [20] El-Demerdash FM, Nasr HM. Antioxidant effect of selenium on lipid peroxidation, hyperlipidemia and biochemical parameters in rats exposed to diazinon. J Trace Elem Med Biol 2014; 28(1): 89–93
  • [21] Hoffmann PR, Berry MJ. The influence of selenium on immune responses. Mol Nutr Food Res 2008; 52:1273–1280
  • [22] Araúz ILC, Afton S, Wrobel K, Caruso JA, Corona JFG, Wrobel K. Study on the protective role of selenium against cadmium toxicity in lactic acid bacteria: An advanced application of ICP-MS. J Hazard Mater 2008; 153: 1157-1164
  • [23] Vinceti M, Filippini T, Cilloni S, Bargellini A, Vergoni AV, Tsatsakis A, Ferrante M. Health risk assessment of environmental selenium: Emerging evidence and challenges. Mol Med Rep 2017; 15(5): 3323-3335.
  • [24] Gutteridge JMC, Halliwell B. The Deoxsiriboz Assay Both for Free Radical and for Site Specific Hydroxyl Radical Production. Biochem 1988; 253-932.
  • [25] Ognjanovic BJ, Pavlovic SZ, Maletic D, Zikic RV, Stajn AS, Radojicic RM, Saicic ZS, Petrovic VM. Protective influence of vitamin E on antioxidant defense system inthe blood of rats treated with cadmium. Physiol Res 2003; 52: 563- 570.
  • [26] Bolander FF. Vitamins: not just for enzymes, Current opinion in investigational drugs (London, England: 2000) 2006; 7(10): 912-915
  • [27] Muhamma I, Ashiru S, Kanoma A, Sani I, Garba S. Effect of ripening stage on vitamin C content in selected fruits. Int J Agric Forestry Fisheries 2014; 2: 60-65
  • [28] Kumar A, Pandey R, Siddiqi N. Oxidative stress biomarkers of cadmium toxicity in mammalian systems and their distinct ameliorative strategy. J Appl Biotechnol Bioeng 2019; 6: 126-135.
  • [29] Gaweł S, Wardas M, Niedworok E, Wardas P. Malondialdehyde as lipid peroxidation marker. Wiad Lek 2004; 57(9-10): 453-455.
  • [30] Schaur RJ, Siems W, Bresgen N, Eckl PM. 4-Hydroxy-nonenal—a bioactive lipid peroxidation product. Biomolecules 2015; 5: 2247-2337.
  • [31] Choudhary M, Jetley UK, Khan MA, Zutshi S, Fatma T. Effect of heavy metal stress on proline, malondialdehyde, and superoxide dismutase activity in the cyanobacterium Spirulina platensis-S5. E Ecotoxicol Environ Saf 2007; 66: 204- 209.
  • [32] Corticeiro SC, Lima AIG, Figueira EMdAP. The importance of glutathione in oxidative status of Rhizobium leguminosarum biovar viciae under Cd exposure. Enzyme Microb Technol 2006; 40: 132-137.
  • [33] Zwolak I. The role of selenium in arsenic and cadmium toxicity: an updated review of scientific literature. Biol Trace Elem Res 2020; 193: 44-63.
There are 33 citations in total.

Details

Primary Language Turkish
Journal Section MBD
Authors

Muhammad Salihu Ibrahim 0000-0002-7535-4140

Meltem Çakmak 0000-0002-6291-863X

Dursun Özer 0000-0002-7225-8903

Fikret Karataş 0000-0002-0884-027X

Sinan Saydam 0000-0003-1531-5454

Project Number FUBAP FF.18.15
Publication Date September 15, 2021
Submission Date June 3, 2021
Published in Issue Year 2021

Cite

APA Ibrahim, M. S., Çakmak, M., Özer, D., Karataş, F., et al. (2021). Kadmiyumla stres oluşturulan Citrobacter freundii’nin vitamin içeriği ve stres belirteçlerine selenyumun etkisi. Fırat Üniversitesi Mühendislik Bilimleri Dergisi, 33(2), 697-707. https://doi.org/10.35234/fumbd.947044
AMA Ibrahim MS, Çakmak M, Özer D, Karataş F, Saydam S. Kadmiyumla stres oluşturulan Citrobacter freundii’nin vitamin içeriği ve stres belirteçlerine selenyumun etkisi. Fırat Üniversitesi Mühendislik Bilimleri Dergisi. September 2021;33(2):697-707. doi:10.35234/fumbd.947044
Chicago Ibrahim, Muhammad Salihu, Meltem Çakmak, Dursun Özer, Fikret Karataş, and Sinan Saydam. “Kadmiyumla Stres oluşturulan Citrobacter freundii’nin Vitamin içeriği Ve Stres belirteçlerine Selenyumun Etkisi”. Fırat Üniversitesi Mühendislik Bilimleri Dergisi 33, no. 2 (September 2021): 697-707. https://doi.org/10.35234/fumbd.947044.
EndNote Ibrahim MS, Çakmak M, Özer D, Karataş F, Saydam S (September 1, 2021) Kadmiyumla stres oluşturulan Citrobacter freundii’nin vitamin içeriği ve stres belirteçlerine selenyumun etkisi. Fırat Üniversitesi Mühendislik Bilimleri Dergisi 33 2 697–707.
IEEE M. S. Ibrahim, M. Çakmak, D. Özer, F. Karataş, and S. Saydam, “Kadmiyumla stres oluşturulan Citrobacter freundii’nin vitamin içeriği ve stres belirteçlerine selenyumun etkisi”, Fırat Üniversitesi Mühendislik Bilimleri Dergisi, vol. 33, no. 2, pp. 697–707, 2021, doi: 10.35234/fumbd.947044.
ISNAD Ibrahim, Muhammad Salihu et al. “Kadmiyumla Stres oluşturulan Citrobacter freundii’nin Vitamin içeriği Ve Stres belirteçlerine Selenyumun Etkisi”. Fırat Üniversitesi Mühendislik Bilimleri Dergisi 33/2 (September 2021), 697-707. https://doi.org/10.35234/fumbd.947044.
JAMA Ibrahim MS, Çakmak M, Özer D, Karataş F, Saydam S. Kadmiyumla stres oluşturulan Citrobacter freundii’nin vitamin içeriği ve stres belirteçlerine selenyumun etkisi. Fırat Üniversitesi Mühendislik Bilimleri Dergisi. 2021;33:697–707.
MLA Ibrahim, Muhammad Salihu et al. “Kadmiyumla Stres oluşturulan Citrobacter freundii’nin Vitamin içeriği Ve Stres belirteçlerine Selenyumun Etkisi”. Fırat Üniversitesi Mühendislik Bilimleri Dergisi, vol. 33, no. 2, 2021, pp. 697-0, doi:10.35234/fumbd.947044.
Vancouver Ibrahim MS, Çakmak M, Özer D, Karataş F, Saydam S. Kadmiyumla stres oluşturulan Citrobacter freundii’nin vitamin içeriği ve stres belirteçlerine selenyumun etkisi. Fırat Üniversitesi Mühendislik Bilimleri Dergisi. 2021;33(2):697-70.