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Krom (III) Klorür Tuzuna Maruz Kalan Saccharomyces cerevisiae’nın Stres Yanıtı

Year 2021, Volume: 33 Issue: 2, 685 - 695, 15.09.2021

Ebru Çöteli , Fikret Karataş , Seher Gür

https://doi.org/10.35234/fumbd.932608

Abstract

Bu çalışmada, iki farklı dozda (10 ve 40 µg/mL) krom III klorür (CrCl3.6H2O) tuzuna maruz kalan maya (Saccharomyces cerevisiae)’ların ortamına 10, 15 ve 20 µg/mL C vitamini katıldı. Daha sonra mayaların 12, 24 ve 36. saat sonra canlılık oranları belirlendi. Bu mayaların A, E, C vitaminleri, glutatyon (GSH ve GSSG) ve malondialdehit (MDA) miktarları HPLC ile tayin edildi.
Artan krom dozu ve süreye bağlı olarak canlılık oranlarında, GSH, A, E ve C vitamini miktarlarında azalma, GSSG ve MDA miktarlarında ise artmaların olduğu belirlendi. Ayrıca artan C vitamini dozuna bağlı olarak canlılık oranları, GSH, A, E ve C vitamini miktarlarında artma, GSSG ve MDA miktarlarında azalmaların olduğu gözlendi. Artan krom dozuyla mayalarda metabolik strese bağlı olarak serbest radikal oluşumunda artış olduğu, bunun indirekt göstergesi olan MDA miktarında artışlar ve GSH/GSSG oranında ise azalmalar olduğu belirlendi.
Tüm bu bulgulardan kromun; serbest radikal oluşumunu artırdığı ve ortama ilave edilen C vitamininin antioksidan özelliğinden dolayı, serbest radikal oluşumunu azalttığı gözlenmiştir.

Keywords

Saccharomyces cerevisiae Krom Vitamin Stres Antiioksidan parametreler

Supporting Institution

Fırat Üniversitesi Bilimsel Araştırma Projesi (FÜBAP)

Project Number

Fırat Üniversitesi Bilimsel Araştırma Projesi (FÜBAP) FF 11.01 nolu proje

Thanks

Fırat Üniversitesi Bilimsel Araştırma Projesi (FÜBAP) 'ne ve dergiye ve makaleye emeği geçen herkese teşekkürler.

References

  • [1] Samson RA, Hocking AD, Pitt JI, King AD. Modern methods in food mycology. Elsevier, Amsterdam. Science Publishers pp 1992; 275–285.
  • [2] Phaff HJ, Mille MW, Mrak EM. The live of yeasts. Harward University Press, Cambridge, 1966; p. 186.
  • [3] Jakobsen M, Norvhus J. Yeast and their possible beneficial and p. negative effects on the quality of dairy products. Int. Dairy Journal 1996; 6: 755-768.
  • [4] Hierro N, Gonzalez A, Mas A, Guillamon JM. New PCR based methods for yeast identification. Journal of Applied Microbiology 2004; 97: 792-801.
  • [5] Kahvecioğlu Ö, Kartal G, Güven A, Timur S. Metallerin çevresel etkileri I-II, İTÜ Metalurji ve Malzeme Mühendisliği Bölümü (Seminer çalışması 24s) 2004.
  • [6] Barnett JA. The taxonomy of the genus Saccharomyces Meyen ex Reess: A Short Review for Nontaxonomists. Yeast 1992; 81: 1-23.
  • [7] Inoue Y, Nomura W, Takeuchi Y, Ohdate T, Tamasu S, Kitaoka A, Kiyokawa Y, Masutani H, Murata K, Wakai Y, Izawa S, Yodoi J. Efficient extraction of thioredoxin from Saccharomyces cerevisiae by ethanol. Appl Environ Microb 2007; 73: 1672-1675.
  • [8] Gaspar LR, Camargo FB, Graneti MD, Mara Campos PMBG. Evaluation of dermatological effects of cosmetic formulations containing Saccharomyces cerevisiae extract and vitamins. Food and Chemical Toxicology 2008; 46-11: 3493- 3500.
  • [9] Piotrowska A, Mlyni K, Siwek A, Dybala M, Opoka W, Poleszak E, Nowak G. Antidepressant-like effect of chromium chloride in the mouse forced swim test: involvement of glutamatergic and serotonergic receptors. Pharmacol Rep 2008; 60 (6): 991–995.
  • [10] Yang L, Chen JP. Biosorption of hexavalent chromium onto raw and chemically modified Sargassum sp. Bioresource Technology In Press 2007.
  • [11] Korhan H, Halipçi HN, Kertmen M, Dığrak M. Saccharomyces cerevisiae Biyokütlesi ile Remazol Navy Blue Boyar Maddesinin Biyosorpsiyonu. KSÜ Doğa Bil. Derg 2012; 15 (3).
  • [12] Çabuk A, Akar T, Kotluk Z, Şaşmaz S. Saccharomyces cerevisiae hücreleri ile ağır metal giderimi ve metal toleransı. Orlab On-Line Microbiyoloji Dergisi 2007; 05-3:1-7.
  • [13] Bailey JE, Ollis DF. Biochemical engineering Fundamentals. 2nd Edition, Mc Graw Hill, Singapore 1986.
  • [14] Jakubowskı W, Bartosz G. 2,7-Dichlorofluorescin oxidation and reactive oxygen species: what does it measure?. Cell Biology International 2000; 24 (10): 757-760.
  • [15] Miller KW, Lorr NA, Yang CS. Simultaneous determination of plazma Retinol α-Tocopherol, Iycopene, α-Carotene and β-Carotene by high performance liquid chromatography. Analytical Biochem 1984; 138: 340-345.
  • [16] Çetinkaya N, Özcan H. Investigation of seasonal variations in cow serum Retinol and β-Carotene by high performance liquid chromatographic method comp. Biochem Physiol 1991; 100 (4): 1003-1008.
  • [17] Cerhata D, Bauerova A, Ginter E. Determination of Ascorbic Acid in blood serum using high-performance liquid chromatography and its correlation with spectrophotometric. Caska-Slov-Farm 1994; 43 (4): 166-168.
  • [18] Karatas F, Karatepe M, Baysar A. Determination of free malondialdehyde in human serum by high performance liquid chromatography. Analytical Biochemistry 2002; 311: 76-79.
  • [19] Dawes P, Dawes E. SGE Chromatography Products Catalog, (pg: 182) 2000.
  • [20] Mertz W. Chromium in human nutrition: a review. J Nutr 1993; 123: 626–633.
  • [21] Guan-Zetic VG, Stehlik-Tomas V, Grba S, Lutilsky L, Kozlek D. Chromium uptake by Saccharomyces cerevisiae and isolation of glucose tolerance factor from yeast biomass. Journal of Biosciences 2001; 26 (2): 217–223.
  • [22] Pekmez M. Oksidatif stres uygulanmış Schızosaccharomyces Pombe’de moleküler çalışmalar, Yüksek Lisans Tezi, İstanbul Üniversitesi Fen Bilimleri Enstitüsü, İstanbul 2004.
  • [23] Nikolai EP, Tatyana VL, Tatyana AK, Lowell PK. Carotenoids as scavengers of free radicals in a Fenton reaction: Antioxidants or pro-oxidants? Free Rad Bio And Med 2001; 31 (3): 398-404.
  • [24] Kireçci OA. Saccharomyces cerevisiae’nın Gelişme Ortamına İlave Edilen Ağır Metallerin (Mn, Mg, Cd, Fe) Bazı Biyokimyasal Parametrelere Etkileri. KSÜ Doğa Bil Derg 2017; 20 (3): 175-184.
  • [25] Thomas MJ. The role of free radicals and antioxidants. Critical Reviews in Food and Sci Nutrition 1995; 35 (1-2): 21-39.
  • [26] Van-Der-Meulen JH, McArdle A, Jackson MJ, Faulkner JA. Contraction-induced injury to the extensor digitorum longus muscles of rats: the role of vitamin E. J Appl Physiol 1997; 83 (3): 817-823.
  • [27] Kaur G, Alam MS, Athar M. Cumene hydroperoxide debilitates macrophage physiology by inducing oxidative stres: Possible protection by α-tocopherol. Chemico- Biological Interactions 2009; 179: 94-102.
  • [28] Sies H, Stahl W, Sundqust AR. Antioxidant function of vitamins. Vitamin E and C, betacarotene and other carotenoidsa. Ann N Y Acad Sci 1992; 669: 7-20.
  • [29] Acharya S, Acharya UR. In vivo lipid peroxidation responses of tissues in lead-induced Swiss mice. Ind Health 1997; 35: 542–544.
  • [30] Hancock RD, Galpin JR, Viola R. Biosynthesis of L-ascorbic acid (vitamin C) by Saccharomyces cerevisiae. FEMS Microbiology Letters 2000; 186: 245-250.
  • [31] Valko M, Morris H, Cronin MT. Metals, toxicity and oxidative stress. Curr Med Chem 2005; 12: 1161–1208.
  • [32] Lushchak VI. Oxidative stress as a component of transition metal toxicity in fish. In: Svensson, E.P. (Ed.), Aquatic Toxicology Research Focus. Nova Science Publishers Inc., Hauppauge, NY, USA (pp. 1–29) 2008.
  • [33] Gonzalez MJ, Miranda-Massari JR, Mora EM, Guzman A, Riordan NH, Riordan HD, Casciari JJ, Jackson JA, Roman Franco A. Orthomolecular oncology review: ascorbic acid and cancer 25 years later. Integrative Cancer Therapies 2005; 4: 32–44.
  • [34] Büyükakyüz N, Altuğ T, Yaltırık M. Kanser proflaksisinde antioksidan maddelerden E vitamini ve selenyumun önemi. Diş Hekimliğinde Klinik Dergi 2000; 12: 136-139.
  • [35] Stephen DWS, Jameison DJ. Glutathione is important antioxidant molecule in the yeast Saccharomyces cerevisiae. FEMS Microbiology Letters 1996; 141: 207-212.
  • [36] Chai YC, Ashraf SS, Rokutan K, Johnston RB, Jr Thomas, JA. Sthiolation of individual human neutrophil proteins including actin by stimulation of the respiratory burst: evidence againist a role for glutathione disulfide. Arch Biochem Biophys 1994; 310: 273-281.
  • [37] Morel Y, Barauki R. Repression of gene expression by oxidative stres. Biochem J 1999; 342: 481-496.
  • [38] Costa V, Moradas-Ferreira P. Oxidative stress and signal transduction in Saccharomyces cerevisiae: insights into ageing, apoptosis and diseases. Molecular Aspects of Medicine 2001; 22: 217-246.

Year 2021, Volume: 33 Issue: 2, 685 - 695, 15.09.2021

Ebru Çöteli , Fikret Karataş , Seher Gür

https://doi.org/10.35234/fumbd.932608

Abstract

Project Number

Fırat Üniversitesi Bilimsel Araştırma Projesi (FÜBAP) FF 11.01 nolu proje

References

  • [1] Samson RA, Hocking AD, Pitt JI, King AD. Modern methods in food mycology. Elsevier, Amsterdam. Science Publishers pp 1992; 275–285.
  • [2] Phaff HJ, Mille MW, Mrak EM. The live of yeasts. Harward University Press, Cambridge, 1966; p. 186.
  • [3] Jakobsen M, Norvhus J. Yeast and their possible beneficial and p. negative effects on the quality of dairy products. Int. Dairy Journal 1996; 6: 755-768.
  • [4] Hierro N, Gonzalez A, Mas A, Guillamon JM. New PCR based methods for yeast identification. Journal of Applied Microbiology 2004; 97: 792-801.
  • [5] Kahvecioğlu Ö, Kartal G, Güven A, Timur S. Metallerin çevresel etkileri I-II, İTÜ Metalurji ve Malzeme Mühendisliği Bölümü (Seminer çalışması 24s) 2004.
  • [6] Barnett JA. The taxonomy of the genus Saccharomyces Meyen ex Reess: A Short Review for Nontaxonomists. Yeast 1992; 81: 1-23.
  • [7] Inoue Y, Nomura W, Takeuchi Y, Ohdate T, Tamasu S, Kitaoka A, Kiyokawa Y, Masutani H, Murata K, Wakai Y, Izawa S, Yodoi J. Efficient extraction of thioredoxin from Saccharomyces cerevisiae by ethanol. Appl Environ Microb 2007; 73: 1672-1675.
  • [8] Gaspar LR, Camargo FB, Graneti MD, Mara Campos PMBG. Evaluation of dermatological effects of cosmetic formulations containing Saccharomyces cerevisiae extract and vitamins. Food and Chemical Toxicology 2008; 46-11: 3493- 3500.
  • [9] Piotrowska A, Mlyni K, Siwek A, Dybala M, Opoka W, Poleszak E, Nowak G. Antidepressant-like effect of chromium chloride in the mouse forced swim test: involvement of glutamatergic and serotonergic receptors. Pharmacol Rep 2008; 60 (6): 991–995.
  • [10] Yang L, Chen JP. Biosorption of hexavalent chromium onto raw and chemically modified Sargassum sp. Bioresource Technology In Press 2007.
  • [11] Korhan H, Halipçi HN, Kertmen M, Dığrak M. Saccharomyces cerevisiae Biyokütlesi ile Remazol Navy Blue Boyar Maddesinin Biyosorpsiyonu. KSÜ Doğa Bil. Derg 2012; 15 (3).
  • [12] Çabuk A, Akar T, Kotluk Z, Şaşmaz S. Saccharomyces cerevisiae hücreleri ile ağır metal giderimi ve metal toleransı. Orlab On-Line Microbiyoloji Dergisi 2007; 05-3:1-7.
  • [13] Bailey JE, Ollis DF. Biochemical engineering Fundamentals. 2nd Edition, Mc Graw Hill, Singapore 1986.
  • [14] Jakubowskı W, Bartosz G. 2,7-Dichlorofluorescin oxidation and reactive oxygen species: what does it measure?. Cell Biology International 2000; 24 (10): 757-760.
  • [15] Miller KW, Lorr NA, Yang CS. Simultaneous determination of plazma Retinol α-Tocopherol, Iycopene, α-Carotene and β-Carotene by high performance liquid chromatography. Analytical Biochem 1984; 138: 340-345.
  • [16] Çetinkaya N, Özcan H. Investigation of seasonal variations in cow serum Retinol and β-Carotene by high performance liquid chromatographic method comp. Biochem Physiol 1991; 100 (4): 1003-1008.
  • [17] Cerhata D, Bauerova A, Ginter E. Determination of Ascorbic Acid in blood serum using high-performance liquid chromatography and its correlation with spectrophotometric. Caska-Slov-Farm 1994; 43 (4): 166-168.
  • [18] Karatas F, Karatepe M, Baysar A. Determination of free malondialdehyde in human serum by high performance liquid chromatography. Analytical Biochemistry 2002; 311: 76-79.
  • [19] Dawes P, Dawes E. SGE Chromatography Products Catalog, (pg: 182) 2000.
  • [20] Mertz W. Chromium in human nutrition: a review. J Nutr 1993; 123: 626–633.
  • [21] Guan-Zetic VG, Stehlik-Tomas V, Grba S, Lutilsky L, Kozlek D. Chromium uptake by Saccharomyces cerevisiae and isolation of glucose tolerance factor from yeast biomass. Journal of Biosciences 2001; 26 (2): 217–223.
  • [22] Pekmez M. Oksidatif stres uygulanmış Schızosaccharomyces Pombe’de moleküler çalışmalar, Yüksek Lisans Tezi, İstanbul Üniversitesi Fen Bilimleri Enstitüsü, İstanbul 2004.
  • [23] Nikolai EP, Tatyana VL, Tatyana AK, Lowell PK. Carotenoids as scavengers of free radicals in a Fenton reaction: Antioxidants or pro-oxidants? Free Rad Bio And Med 2001; 31 (3): 398-404.
  • [24] Kireçci OA. Saccharomyces cerevisiae’nın Gelişme Ortamına İlave Edilen Ağır Metallerin (Mn, Mg, Cd, Fe) Bazı Biyokimyasal Parametrelere Etkileri. KSÜ Doğa Bil Derg 2017; 20 (3): 175-184.
  • [25] Thomas MJ. The role of free radicals and antioxidants. Critical Reviews in Food and Sci Nutrition 1995; 35 (1-2): 21-39.
  • [26] Van-Der-Meulen JH, McArdle A, Jackson MJ, Faulkner JA. Contraction-induced injury to the extensor digitorum longus muscles of rats: the role of vitamin E. J Appl Physiol 1997; 83 (3): 817-823.
  • [27] Kaur G, Alam MS, Athar M. Cumene hydroperoxide debilitates macrophage physiology by inducing oxidative stres: Possible protection by α-tocopherol. Chemico- Biological Interactions 2009; 179: 94-102.
  • [28] Sies H, Stahl W, Sundqust AR. Antioxidant function of vitamins. Vitamin E and C, betacarotene and other carotenoidsa. Ann N Y Acad Sci 1992; 669: 7-20.
  • [29] Acharya S, Acharya UR. In vivo lipid peroxidation responses of tissues in lead-induced Swiss mice. Ind Health 1997; 35: 542–544.
  • [30] Hancock RD, Galpin JR, Viola R. Biosynthesis of L-ascorbic acid (vitamin C) by Saccharomyces cerevisiae. FEMS Microbiology Letters 2000; 186: 245-250.
  • [31] Valko M, Morris H, Cronin MT. Metals, toxicity and oxidative stress. Curr Med Chem 2005; 12: 1161–1208.
  • [32] Lushchak VI. Oxidative stress as a component of transition metal toxicity in fish. In: Svensson, E.P. (Ed.), Aquatic Toxicology Research Focus. Nova Science Publishers Inc., Hauppauge, NY, USA (pp. 1–29) 2008.
  • [33] Gonzalez MJ, Miranda-Massari JR, Mora EM, Guzman A, Riordan NH, Riordan HD, Casciari JJ, Jackson JA, Roman Franco A. Orthomolecular oncology review: ascorbic acid and cancer 25 years later. Integrative Cancer Therapies 2005; 4: 32–44.
  • [34] Büyükakyüz N, Altuğ T, Yaltırık M. Kanser proflaksisinde antioksidan maddelerden E vitamini ve selenyumun önemi. Diş Hekimliğinde Klinik Dergi 2000; 12: 136-139.
  • [35] Stephen DWS, Jameison DJ. Glutathione is important antioxidant molecule in the yeast Saccharomyces cerevisiae. FEMS Microbiology Letters 1996; 141: 207-212.
  • [36] Chai YC, Ashraf SS, Rokutan K, Johnston RB, Jr Thomas, JA. Sthiolation of individual human neutrophil proteins including actin by stimulation of the respiratory burst: evidence againist a role for glutathione disulfide. Arch Biochem Biophys 1994; 310: 273-281.
  • [37] Morel Y, Barauki R. Repression of gene expression by oxidative stres. Biochem J 1999; 342: 481-496.
  • [38] Costa V, Moradas-Ferreira P. Oxidative stress and signal transduction in Saccharomyces cerevisiae: insights into ageing, apoptosis and diseases. Molecular Aspects of Medicine 2001; 22: 217-246.
There are 38 citations in total.

Details

Primary Language Turkish
Subjects Engineering
Journal Section MBD
Authors

Ebru Çöteli 0000-0002-9473-0914

Fikret Karataş 0000-0002-0884-027X

Seher Gür 0000-0003-0081-5990

Project Number Fırat Üniversitesi Bilimsel Araştırma Projesi (FÜBAP) FF 11.01 nolu proje
Publication Date September 15, 2021
Submission Date May 4, 2021
Published in Issue Year 2021 Volume: 33 Issue: 2

Cite

APA Çöteli, E., Karataş, F., & Gür, S. (2021). Krom (III) Klorür Tuzuna Maruz Kalan Saccharomyces cerevisiae’nın Stres Yanıtı. Fırat Üniversitesi Mühendislik Bilimleri Dergisi, 33(2), 685-695. https://doi.org/10.35234/fumbd.932608
AMA Çöteli E, Karataş F, Gür S. Krom (III) Klorür Tuzuna Maruz Kalan Saccharomyces cerevisiae’nın Stres Yanıtı. Fırat Üniversitesi Mühendislik Bilimleri Dergisi. September 2021;33(2):685-695. doi:10.35234/fumbd.932608
Chicago Çöteli, Ebru, Fikret Karataş, and Seher Gür. “Krom (III) Klorür Tuzuna Maruz Kalan Saccharomyces cerevisiae’nın Stres Yanıtı”. Fırat Üniversitesi Mühendislik Bilimleri Dergisi 33, no. 2 (September 2021): 685-95. https://doi.org/10.35234/fumbd.932608.
EndNote Çöteli E, Karataş F, Gür S (September 1, 2021) Krom (III) Klorür Tuzuna Maruz Kalan Saccharomyces cerevisiae’nın Stres Yanıtı. Fırat Üniversitesi Mühendislik Bilimleri Dergisi 33 2 685–695.
IEEE E. Çöteli, F. Karataş, and S. Gür, “Krom (III) Klorür Tuzuna Maruz Kalan Saccharomyces cerevisiae’nın Stres Yanıtı”, Fırat Üniversitesi Mühendislik Bilimleri Dergisi, vol. 33, no. 2, pp. 685–695, 2021, doi: 10.35234/fumbd.932608.
ISNAD Çöteli, Ebru et al. “Krom (III) Klorür Tuzuna Maruz Kalan Saccharomyces cerevisiae’nın Stres Yanıtı”. Fırat Üniversitesi Mühendislik Bilimleri Dergisi 33/2 (September 2021), 685-695. https://doi.org/10.35234/fumbd.932608.
JAMA Çöteli E, Karataş F, Gür S. Krom (III) Klorür Tuzuna Maruz Kalan Saccharomyces cerevisiae’nın Stres Yanıtı. Fırat Üniversitesi Mühendislik Bilimleri Dergisi. 2021;33:685–695.
MLA Çöteli, Ebru et al. “Krom (III) Klorür Tuzuna Maruz Kalan Saccharomyces cerevisiae’nın Stres Yanıtı”. Fırat Üniversitesi Mühendislik Bilimleri Dergisi, vol. 33, no. 2, 2021, pp. 685-9, doi:10.35234/fumbd.932608.
Vancouver Çöteli E, Karataş F, Gür S. Krom (III) Klorür Tuzuna Maruz Kalan Saccharomyces cerevisiae’nın Stres Yanıtı. Fırat Üniversitesi Mühendislik Bilimleri Dergisi. 2021;33(2):685-9.
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