Abstract
In this study, physical stability of omega-3 oil-in-water emulsion was investigated at different environmental conditions. Omega-3 oil (5%) and Tween 80 (T80), Quillaja Saponin (QS), Gam Arabic (GA) (1%) were used to produce oil-in water nanoemulsions. The physical stability of nanoemulsions were examined at different pH (4-7), temperature (40-80oC) and ionic strength (0-500 mM NaCl). The particles size of nanoemulsions were 125±0.40 to 542±1.70 nm, and the charge were −12.80±0.63 and −69.10±0.14 mV. It was observed that nanoemulsions containing T80 and QS were kinetically stable and the particle size/zeta potential did not change at different pH, temperature and salt concentrations. However, there was a small changes in the particle size of nanoemulsion containing GA at different conditions. As a result, physically stable nanoemulsions enriched with omega-3 were produced using both synthetic and natural surfactants. These results will be useful for the production of food enriched with omega-3 oils