Chemometric Optimization of Ultrasonic Assisted Extraction Parameters of Antioxidants from White Chicory (Cichorium intybus L.)

Year 2023, Volume: 13 Issue: 3, 553 - 565, 15.07.2023

Açelya Aklan , Önder Aybastıer

https://doi.org/10.17714/gumusfenbil.1239972

Cited By: 1

Abstract

In order to optimize the experimental conditions for the ultrasonic assisted extraction of phenolic compounds from chicory leaves, a five level five factor central composite design response surface methodology was used. Total phenolic content and antioxidant capacity were selected as the response and 48 experiments were performed using five independent variables; time (min), HCl concentration (mol/L), temperature (ºC), liquid/solid ratio (mL/g) and methanol concentration (%). As a result of optimization, optimum conditions, for maximum total phenolic content were determined as temperature 73 ºC, time 69 min, methanol 84%, HCl concentration 0.57 mol/L and liquid/solid ratio 33.97 mL/g. The optimum conditions, for maximum antioxidant capacity were temperature 73 ºC, time 69 min, methanol 85%, HCl concentration 0.47 mol/L and liquid/solid ratio 33.99 mL/g. The total phenolic content of white chicory extract obtained as a result of extraction under optimum conditions was determined as 33.5±0.5 mg GAE/g dried plant and antioxidant capacity as 7.2±0.1 mg TE/g dried plant.

Keywords

Antioxidant capacity, Cichorium intybus L., Phenolic substance, Chemometric optimization, Response surface methodology

Beyaz hindibadan (Cichorium intybus L.) antioksidan maddelerin ultrasonik destekli ekstraksiyon parametrelerinin kemometrik optimizasyonu

Abstract

Beyaz hindiba yapraklarından fenolik bileşiklerin ultrasonik destekli ekstraksiyonu için deneysel koşulları optimize etmek amacıyla beş seviyeli beş faktörlü yanıt yüzey yöntemi kullanılmıştır. Yanıt verileri olarak toplam fenolik madde ve antioksidan kapasite seçilerek beş bağımsız değişken; süre (dk), HCl derişimi (mol/L), sıcaklık (ºC), sıvı/katı oranı (mL/g) ve metanol derişimi (%) kullanılarak 48 deney gerçekleştirilmiştir. Optimizasyon sonucunda en yüksek toplam fenolik madde için optimum koşullar, sıcaklık 73 ºC, süre 69 dk, metanol derişimi %84, HCl derişimi 0.57 mol/L ve sıvı/katı oranı 33.97 mL/g olarak belirlenmiştir. En yüksek antioksidan kapasite için optimum koşullar sıcaklık 73 ºC, süre 69 dk, metanol derişimi %85, HCl derişimi 0.47 mol/L ve sıvı/katı oranı 33.99 mL/g olarak belirlenmiştir. Belirlenen optimum koşullarda gerçekleştirilen ekstraksiyon sonucunda elde edilen beyaz hindiba ekstraktının toplam fenolik madde içeriği 33.5±0.5 mg GAE/g kuru bitki ve antioksidan kapasite 7.2±0.1 mg TE/g kuru bitki olarak bulunmuştur.

Keywords

Antioksidan kapasite, Cichorium intybus L, Fenolik madde, Kemometrik optimizasyon, Yanıt yüzey yöntemi

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