Effects of different BA and IBA concentrations on proliferation and rooting of ‘GARNEM’ rootstock in vitro propagation

Year 2021, Volume: 5 Issue: 4, 470 - 476, 15.12.2021

Bekir Erol Ak , Pınar Karahan Kıyar Ibrahim Halil Hatipoglu , Birgül Dikmetaş

https://doi.org/10.31015/jaefs.2021.4.6

Cited By: 2

Abstract

In this study, the regeneration shoot tip and nodal explants grown in vitro conditions of ‘Garnem’ hybrid rootstock were investigated, the effect of different phytohormone and concentrations on obtaining adventitious shoots from different explants was investigated, so an efficient protocol was developed for in vitro regeneration of ‘Garnem’ hybrid rootstock These outputs of the study can be a reference resource for future in vitro and biotechnological studies on the rootstock in question. The differences of PGR in MS medium culture containing node explants infinite (0.5-2.0 mg / l) BA (benzyladenine) were investigated. Upon the research, it was observed that the number of shoots andproliferationwere achieved in explants of nodal cuttings that were taken to culture in MS medium containing 2.0 mg / l BA. It has been determined that the most effective culture medium for the elongation of shoot length is MS medium containing 0.5 mg / l BA, 30 g / l sucrose and 5.5 g / l agar. Shoots growing in length were transferred to a new culture with ½ MS medium 0.5-2.0 mg / l IBA (indole-3-butyric acid) to be rooted. While rooting at a rate of 42.8% was achieved in ½ MS medium containing 2 mg / l IBA, 47.2% of rooted plantlets were acclimatized to ex- vitro conditions. Rooted plantlets obtained under in vitro conditions were transferred to plastic containers with 3 different environments in order to get accustomed to external conditions. At the end of the 8th week, the vitality rates of the plantlets were determined. While the viability rate of the plantlets transferred to the medium containing peat: perlite at the ratio of 1: 1 was found to be 47.2%, the viability rate of the plantlets in the environment containing only perlite was found to be 32.8%, and the viability rate of the plantlets in the environment containing only peat was found as 23.6%

Keywords

‘‘Garnem’’, rootstock, woody plants, regeneration, in vitro, micropropagation

Project Number

15022

References

• Ak, B.E. (2018). The Importance of in vitro micropropagation of fruit crops. 1st International GAP Agriculture and Livestock Congress Proceedings Book. (ISBN 978-975-7113-65-2): 716-723.
• Ainsley P. J., Collins G. G., Sedgley, M. (2000). Adventitious shoot regeneration from leaf explants of almond (Prunus dulcis Mill.). In vitro Cellular and Development Biology-Plant, 36(6): 470-474.
• Ainsley, P. J., Hammerschlag, F. A., Bertozzi, T., Collins, G. G., Sedgley, M. (2001a). Regeneration of almond from immature seed cotyledons. Plant Cell, Tissue and Organ Culture, 67: 221-226. DOI: https://doi.org/10.1023/A:1012700714085
• Ainsley, P. J., Collins, G. G. and Sedgley, M. (2001b). In vitro rooting of almond (Prunus dulcis Mill.). In vitro Cellular and Development Biology Plant, 37:778-785. DOI: https://doi.org/10.1007/s11627-001-0129-4
• Akcay, M.E., Tosun, I. (2005). Bazı geç çiçek açan yabancı badem çesitlerinin Yalova ekolojik kosullarındaki gelişme ve verim davranışları. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 36 (1): 1-5 (In Turkish).
• Antonopoulou, C., Dimassi, K., Therios, I., Chatzissavvidis, C., Tsırakoglou, V. (2005). Inhibitory effects of riboflavin (Vitamin B2) on the in vitro rooting and nutrient concentration of explants of peach rootstock GF 677 (P. amygdalus x P. persica). Scientia Horticulturae, 106: 268-272. DOI: https://doi.org/10.1016/j.scienta.2005.02.019
• Arici, S. E. (2008). Bazı sert çekirdekli meyve anaçlarının doku kültürü ile çogaltılması. Süleyman Demirel Üniversitesi Ziraat Fakültesi Dergisi, 3(1): 19-23 (In Turkish).
• Babaoglu, M., Gürel, E., Özcan, S. (2002). Bitki biyoteknolojisi. Selcuk Üniversitesi Vakfi Yayinlari, 374s (In Turkish).
• Channuntapipat, C., Sedgley, M., Collins, G. (2003). Micropropagation of almond cultivars nonpareil and ne plus ultra and the hybrid rootstock Titan×Nemaguard. Scientia Horticulturae,98: 473–484. DOI: https://doi.org/10.1016/S0304-4238(03)00067-0
• Durkovic, J. (2006). Rapid micropropagation of mature wild cherry. Biologia Plantarum, 50 (4): 733-736. DOI: https://doi.org/10.1007/s10535-006-0118-x
• Eldogan, Ü. (2020). Dünya’da ve Türkiye’de Badem. Antepfıstığı Araştırma Dergisi, 44-45. (In Turkish).
• Espinosa, A. C., Pijut, P. M., Michler, C. H. (2006). Adventitious shoot regeneration and rooting of Prunus serotina in vitro Cultures. Hortscience, 41(1): 193-201. DOI: https://doi.org/10.21273/HORTSCI.41.1.193
• Fotopoulos, S. and Sotiropoulos, T. E. (2005). In vitro rooting of PR 204/84 rootstock as influenced by mineral concentration of the culture medium and exposure to darkness for a period. Agronomy Research, 3(1): 3-8.
• Gürel, S., Gülsen, Y. (1998). The effects of IBA and BAP on in vitro shoot production of almond (Amygdalus communis L.). Tr.J. of Botany, 22: 375-379.
• Jain, N., Babbar, S. B. (2000). recurrent production of plants of black plum Syzygium cuminii (L.) skeels, a myrtaceous fruit tree, from in vitro cultured seedling explants. Plant Cell Reports, 19: 519-524. DOI: https://doi.org/10.1007/s002990050766
• Jain, N., Babbar, S.B. (2003). Regeneration of “Juvenile” plants of black plum, Syzygium cuminii skeels, from nodal exp of mature trees. Plant Cell, Tissue and Organ Culture, 73: 257-263. DOI: https://doi.org/10.1023/A:1023039510659
• Matt, A., Jehle, J.A. (2005). In vitro plant regeneration from leaves and internode sections of sweet cherry cultivars (Prunus avium L.) Plant Cell Rep., 24: 468-476. DOI: https://doi.org/10.1007/s00299-005-0964-6
• Muna, A.S., Ahmad, A.K., Mahmoud , K., Abdul-Rahman, K. (1999.) In vitro propagation of a semi-drawting cherry rootstock. Plant Cell and Tissue and Organ Culture, 59: 203- 208. DOI: https://doi.org/10.1023/A:1006444925445
• Osterc, G., Luthar, Z., Stampar, F. (2004). The importance of the sterilization procedure for producing vigorous cherry plants (Prunus sp.) in vitro. Acta Agriculturae Slovenica, 83: 45-51.
• Ozden, A.N., Ak, B.E., Ozden, M. (2011). In vitro regeneration and micropropagation of GF-677 Hybrid (Prunus amygdalus x P. persica) rootstock. ActaHorticulturae, 912(2): 653-656. DOI: https://doi.org/10.17660/ActaHortic.2011.912.98
• Pruski, K., Astatkie, T., Nowak, J. (2005). Tissue culture propagation of mongolian cherry (Prunus fruticosa) and nanking cherry (Prunus tomentosa). Plant Cell, Tissue and Organ Culture, 82: 207-211. DOI: https://doi.org/10.1007/s11240-004-7836-6
• Socias, I., Company, R. (1998). La taxonomie de 1’amandier. Options Meditererraneennes, 33: 91-93. (In French).