Application of multiple strategies to efficiently break seed dormancy of permanently odd pentaploid rose hip (Rosa canina l.) under in vitro conditions

Year 2022, Volume: 6 Issue: 3, 471 - 479, 23.09.2022

Mortaza Hajyzadeh

https://doi.org/10.31015/jaefs.2022.3.17

Abstract

Rosehip (Rosa canina L.) is an important medicinal, and ornamental plant species with high commercial value. Its sugars, phenolics, organic acids, water-soluble vitamins, and mineral contents composition varies depending on environmental conditions and genetics. The plant is also used as a perfect rootstock for many rose cultivars. Seed proliferation is extremely difficult because of multiple endogenous and exogenous dormancy factors. There is a need to breed standard rosehip cultivars rootstock developments with desired characteristics and outperforming yields in fields. The study aimed to break the seed dormancy of rosehip under in vitro conditions by application of multiple strategies in an efficient manner. The seeds were treated with different doses of GA₃, scarified mechanically, stratified on agar solidified MS medium containing GA₃ singly or in combinations of the two each or three treatments, and subjecting them to the regulated physiological treatment of alternating warm/chilling and cold/dark treatments in parallel for 21 d ensued by 18 d warm/light treatments. It was noted that the rosehip seeds could be germinated variably if the scarified seeds were stratified on agar solidified MS medium with or without GA3. Optimum seed germination (80.00- 85.00%) was noted when the three treatments were combined and the seeds were subjected to regulated and alternating warm and chilling treatments for 21 d leaving them for 18 d in warm/light. These results are very important and could be utilized in breeding and multiplication programs to develop new rosehip fruit and rootstock cultivars.

Keywords

Breeding, Diversity, Germination, Scarification, Stratification

References

• Acharya, S.N., Kastelıc, J.P., Beauchemın, K.A. and Messenger, D.F. (2006). A review of research progress on cicer milkvetch (Astragalus cicer L.). Canadian Journal of Plant Science 86(1):49–62. DOI: https://doi.org/10.4141/P04-174
• Albrecht, M.A. and Penzagos, J.C.Z. (2012). Seed germination ecology of three imperiled plants of rock outcrops in the southeastern United States. Journal of the Torrey Botanical Society. 139(1):86–95 DOI: https://doi.org/10.3159/TORREY-D-11-00066.1
• Arora, R., Rowland, L.J. and Tanino, K. (2003). Induction and release of bud dormancy in woody perennials: a science comes of age. HortScience 38(5):911-921.
• Balta, F. and Cam, I. (1996). Some fruit characteristics of Rosehips types selected from gevas and Ahlat districts-I. J. Agric. Sci., 6: 155-160.
• Bewley, J.D. and Black, M. (1994). Seeds: Physiology of Development and Germination. Second Edition. Plenum Press.
• Blackburn, K.B. (1925). Chromosomes and classification in the genus Rosa. Am Nat, 59:200-204. 12.
• Blackburn, K. and Harrison, J.H.W. (1921). The status of the British rose forms as determined by their cytological behaviour. Ann Bot 1921, 35(138):159-188. 11.
• Christensen, R., Tarp, S. and Altman, R.D. (2014). Comparing different preparations and doses of rosehip powder in patients with osteoarthritis of the knee: an exploratory randomized active-controlled trial. Int J Clin Rheumatol. 9(3):267–278.
• Claessens, S.M. (2012). Dormancy cycling in seeds: mechanisms and regulation. Ph.D. thesis, Wageningen University, The Netherlands.
• Daneshvar, A., Tigabu, M., Karimidoost, A. and Odén, P. C. (2017). Flotation techniques to improve viability of Juniperus polycarpos seed lots. Journal of forestryresearch, 28(2), 231-239.
• Davoudi Pahnekolayi, M., Tehranifar, A., Samiei, L., and Shoor, M. (2019). Optimizing culture medium ingredients and micrografting devices can promote in vitro micrografting of cut roses on different rootstocks. Plant Cell, Tissue and Organ Culture (PCTOC), 137(2), 265-274.
• Demir, F. and Ozcan, M. (2001). Chemical and technological properties of rose (Rosa canina L.) fruits grown in Turkey. J. Food Eng., 47: 333-336.
• Duru, N., Karadeniz, F. and Erge, H.S. (2012). Changes in bioactive compounds, antioxidant activity and HMF formation in rosehip nectars during storage. Food and Bioprocess Technology, 5(7), 2899- 2907. http://dx.doi.org/10.1007/s11947-011-0657-9
• Ercisli, S. (2005). Rose (Rosa spp.) germplasm resources of Turkey. Genet Resour Crop Eval., 52:787–795.
• Ercisli, S. (2007). Chemical composition of fruits in some rose (Rosa spp.) species. Food Chem. 104: 1379–1384.
• Grbic, M., Mijanovic, O. and Jevtic, S. (1996). Different presowing treatments of Rosa canina 'Schmidts ideal' seeds and their impact on production cycle shortening. Acta Hortic. 424, 99-102. DOI: https://doi.org/10.17660/ActaHortic.1996.424.14
• Groot, S.P.C. and Karssen, C.M. (1992). Dormancy and germination of abscisic acid deficient tomato seeds: Studies with the sitiens mutant. Plant Physiol. 99, 952-958.
• Gudin, S. 2001. Rose breeding technologıes. Acta Hortic. 547, 23-33. https://doi.org/ 10.17660/ActaHortic.2001.547.2
• Gudin, S., Arene, L., Chavagnat, A. and Bulard, C. (1990). Influence of endocarp thickness on rose achene germination: genetic and environmental factors. HortScience 25:786- 788.
• Hajian, S., and Khosh-Khui, M. (2000). Investigation on sexual and asexual propagation methods of Damask rose (Rosa damascena Mill.). Iran Agric. Res. 19(1): 1-16.
• Haouala, F., Hajlaoui, N. and Cheikh-Affene, Z.B. (2013). Enhancing Seed Germination in Rose (Rosa rubiginosa L.). Medicinal and Aromatic Plants, http://dx.doi.org/10.4172 /2167-0412.1000139
• Heide, O. M. (2008). Interaction of photoperiod and temperature in the control of growth and dormancy of Prunus species. Scientia Horticulturae. 115:309–314.
• Hosafci, H., Arslan, N. and Sarihan, E.O. (2005). Propagation of dog roses (Rosa canina L.) by seed. Acta Hortic. 690, 159-164.
• Iakovoglou, V. and Radoglou, K. (2015). Breaking seed dormancy of three orthodox Mediterranean Rosaceae species. J. Environ Biol. 36(2):345-9.
• ISTA, (2019). https://www.ingentaconnect.com/content/ista/rules/2019/00002019/00000001 (accessed 28.012019).
• Karssen, C.M. (1995). Hormonal regulation of seed development, dormancy, and germination studied by genetic control. In Seed Development and Germination, J. Kigel and G. Galili, eds. (New York: Marcel Dekker, Inc.), pp. 333-350.
• Karssen, C.M., Brinkhorst-van der Swan, D. L. C., Breekland, A. E. and Koomneef M. (1983). Induction of dormancy during seed development by endogenous abscisic acid: studies on abscisic acid deficient genotypes of Arabidopsis thaliana (L.) Heynh. Planta 157, 158-165.
• Kazaz, S., Erbaş, S. and Baydar, H. (2010). Breaking seed dormancy in oil rose (Rosa damascene Mill.) by microbial inoculation. African J. Biotechnol. 9, 6503-6508.
• Kim, T. H., Yoon, C., and Kim, J. H. (2021). The Report on the Taxonomic Characters, Ecological Risk and Weed Risk Assessment of Putative Invasive Alien Plants which are Designated in Law by the Ministry of Environment in Korea as Environmentally Harmful Species (III). Korean Journal of Plant Resources, 34(3), 223-248.
• Kochanek, J. (2018). On Farm Evaluation of Vegetable Seed Viability Using Non Destructive Techniques.
• Koornneef, M., Bentsink, L. and Hilhorst, H. (2002). Seed dormancy and germination. Curr Opin Plant Biol. 5(1):33-6.
• Koornneef, M., Reuling, G. and Karssen, C.M. (1984). The isolation and characterization of abscisic acid-insensitive mutants of Arabidopsis thaliana. Physiol. Plant 61, 377-383.
• Leubner, G. (2010). Seed dormancy. The Seed Biology Place. www.seedbiology.de/dormancy.asp , 11p.
• Lim, K. Y., Werlemark, G., Matyasek, R., Bringloe, J.B., Sieber, V., El Mokadem, H. and Roberts, A.V. (2005). Evolutionary implications of permanent odd polyploidy in the stable sexual, pentaploid of Rosa c anina L. Heredity, 94(5), 501-506.
• Mármol, I., Sánchez-de-Diego, C., Jiménez-Moreno, N., Ancín-Azpilicueta, C., & Rodríguez-Yoldi, M. J. (2017). Therapeutic applications of rose hips from different Rosa species. International journal of molecular sciences, 18(6), 1137.
• McDonald, M.B., Marcos-Filho, J. and Bino, R.J. (1998). Seed technology training in the year 2000. Sci. agric., Piracicaba 55 (Número Especial), 1-5.
• Meyer, S. E. (2008). Rosa L. rose, briar. In: Bonner FT, Karrfalt RP (eds.) The Woody Plant Seed Manual, Agriculture Handbook No. 727, pp. 974–980. Washington, DC: US Department of Agriculture, Forest Service.
• Nadeem, M., Riaz, A., Younis, A., Akond, M., Farooq, A. and Tariq, M. (2013). Improved technique for treating seed dormancy to enhance germination in Rosa × hybrid. Turk J Bot (2013) 37: 521-529.
• Nadjafi, F., Bannayan, M., Tabrizi, T. and Rasgoo, M. (2006). Seed germination and dormancy breaking techniques for Ferula gummasa and Teucrium polium. Journal of Arid Enviroments 64, 542-547.
• Nagatomo, A., Nishida N. and Fukuhara I. (2015). Daily intake of rosehip extract decreases abdominal visceral fat in preobese subjects: a randomized, double-blind, placebo-controlled clinical trial. Diabetes Metab Syndr Obes. 8:147–156.
• Nilsson, O. (1997). In: Flora of Turkey and the East Aegean Islands. 4. Davis PH, editor. Edinburgh: Edinburgh University Press; Rosa; pp. 106–128.
• Nishimoto, R.K. and McCarty, L.B. (1997). Fluctuating temperature and light influence seed germination of gosegrass (Eleusine indica). Weed Sci.;45:426- 429.
• Nitsch, J.P. (1957). Photoperiodism in woody plants. Proceedings of the American Society for Horticultural Science. 70:526–544.
• Ouyang, L., Leus, L., and Van Labeke, M. C. (2019). Three-year screening for cold hardiness of garden roses. Scientia Horticulturae, 245, 12-18.
• Porter, R., Durrell, M. and Romm, H. (1947). The use of 2,3,5-triphenyl tetrazolium chloride as a measure of seed germinability. Plant Physiol. 22 (2), 149.
• Prevost, I. and Le Page-Degivry, M.T. (1985). Inverse correlation between ABA content and germ inability throughout the maturation and the in vitro culture of the embryo of Phaseolus vulgaris. J. Exp. Bot. 36, 1457-1464.
• Pugazhendhi, A., Vasantharaj, S., Sathiyavimal, S., Raja, R.K., Karuppusamy, I., Narayanan, M., Brindhadevi, K. (2021). Organic and inorganic nanomaterial coatings for the prevention of microbial growth and infections on biotic and abiotic surfaces. Surface and Coatings Technology, 425, 127739.
• Quave, C.L., Plano, L. R.W., Pantuso T. and Bennet, B.C. (2008). Effects of extracts from Italian medicinal plants on planktonic growth, biofilm formation and adherence in MRSA. J Ethnopharmacol. 118:418–428.
• Rakhimova, T., Rakhimova, N.K., Shomurodov, K.F., and Abduraimov, O.S. (2020). Ontogenetic structure of rare plant species on the Usturt Plateau in Uzbekistan. Arid Ecosystems, 10(3), 238-243.
• Ritz C.M., Schmuths, H., Wissemann, V. (2005). Evolution by reticulation: European dogroses originated by multiple hybridizations across the genus Rosa. J Hered 96:4-14.
• Selahvarzian, A., Alizadeh, A., Baharvand, P.A., Eldahshan, O.A., and Rasoulian, B. (2018). Medicinal Properties of Rosa canina L. Herbal Medicines Journal (Herb Med J), 77-84.
• Semeniuk, P. and Stewart, R.N. (1966). Effect of temperature and duration of after ripening period on germination of Rosa nutkana seeds. Proc. Am. Soc. Hort. Sci. 89, 689–693.
• Semeniuk, P. and Stewart, R.N. (1970). Temperature reversal of after-ripening of rose seeds. RHA Newsletter 2(7):16-18.
• Sonsteby, A. and Heide, O. M. (2010). Earliness and fruit yield and quality of annual-fruiting red raspberry (Rubus idaeus L.): Effects of temperature and genotype. Journal of Horticultural Science and Biotechnology, 85, 341–349.
• Söyler, D., Canihoş, E., Temel, N. and Hajyzadeh, M. (2012). Determination of chemical fungicide against soil borne fungal diseases of capers (Capparis ovata Desf.var. herbacea) during early stages. Pak. J. Agri. Sci., Vol. 49(3), 345-348, ISSN (Print) 0552-9034, ISSN (Online) 2076-0906.
• Stewart, R.N. and Semeniuk, P. (1965). The effect of the interaction of temperature with after-ripening requirements and compensating temperature on germination of seeds of 5 species of Rosa. Am. J. Bot. 52: 755-760.
• Sumlu, S., Atar, H.H. and Khawar, K.M. (2010). Breaking seed dormancy of water lily (Nymphaea aiba L.) under in vitro conditions. Biotechnology and Biotechnological Equipment. 24(1), pp. 1582-1586.
• Svejda, F. (1968). Effect of temperature and seed coat treatment on the germination of rose seeds, HortScience 3, 184–185.
• Svejda, F. (1972). Water uptake of rose achenes. Can. J. Plant Sci. 52:1043-1047.
• Täckholm G. (1920). On the cytology of the genus Rosa. A preliminary note. Svensk Bot Tidskr 1920, 14(2-3):300-311.
• Tavşanoğlu, Ç., Kazancı, D. D., Soyumert, A., Ertürk, A., & Değirmenci, C. Ü. (2021). Seed dispersal by the brown bear in a mixed temperate forest: fruit type matters. Mammal Research, 66(1), 137-147.
• Thomas, B. and Vince-Prue,D. (1997). Photoperiodism in plants, London Academic Press.
• Venkatesha, K. T., Gupta, A., Rai, A. N., Jambhulkar, S. J., Bisht, R., & Padalia, R. C. (2022). Recent developments, challenges, and opportunities in genetic improvement of essential oil-bearing rose (Rosa damascena): A review. Industrial Crops and Products, 184, 114984.
• Walker-Simmons, M. (1987). ABA levels and sensitivity in developin g wheat embryos of sprouting resistant and susceptible cultivars. Plant Physiol. 84, 61-66.
• Werlemark, G. (2009). Dogrose: Wild Plant, Bright Future. Chronica Horticulturae 49(2): 8-13.
• Werlemark, G., Carlson-Nilsson, U. Uggla, M. and Nybom, H. (1995). Effects of Temperature Treatments on Seedling Emergence in Dogroses, Rosa Sect. Caninae (L). Acta Agriculturae Scandinavica. Section B, Soil Plant Sciences, 45(4): 278-282.
• Zeven, Anton C.and De Wet Jan M. J. (2020). Dictionary of cultivated plants and their regions of diversity: excluding most ornamentals, forest trees and lower plants. Pudoc, 1982. Retrieved from https://edepot.wur.nl/350203
• Zhong, L. (2017). Anti-cancer effects of bioactive compounds from rose hip fruit in human breast cancer cell lines.
• Zhou, Z.Q., Wei-Kai, B. and Ning, W. (2009). Dormancy and germination in Rosamulti bracteata Hemsl and E.H. Wilson. Sci. Hortic. 119: 434- 441.
• Zhou, Z.Q., Wei-Kai, B. and Ning, W. (2009). Dormancy and germination in Rosa multibracteata Hemsl and E.H. Wilson. Sci. Hortic. 119:434- 441.
• Zlesak, D.C. (2007). Rose. In: Anderson N.O. (ed.), Flower Breeding and Genetics, pp. 695-740.